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1994 Fiscal Year Final Research Report Summary

Purification OF THCA synthase from Cannabis callus

Research Project

Project/Area Number 05671830
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MORIMOTO Satoshi  Kyusyu University, Faculty of pharmaceutical Sciences Associate Professor, 薬学部, 助教授 (60191045)

Co-Investigator(Kenkyū-buntansha) SHOYAMA Yukihiro  Kyusyu University, Faculty of pharmaceutical Sciences Professor, 薬学部, 教授 (70037604)
Project Period (FY) 1993 – 1994
KeywordsCannabis sativa / Cannabinoid / Tetrahydrocannabinolic acid / Tetrahydrocannabinolic acid synthase
Research Abstract

The occurrence of DELTA^1-tetrahydrocannabinolic acid synthase was confirmed in the fresh leaf buds of marihuana (Cannabis sativa L.). The enzyme, assayd with cannabigerolic acid, was purified approximately 300-fold by chromatography on diethylaminoethyl-cellulose, phenyl Sepharose CL-4B and hydroxylapatite. The purified protein migrated during sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass of 75kD.The native molecular mass determined by gel-filtration chromatography was 74 kDa, indicating that DELTA^1-tetrahydrocannabinolic acid synthase is a monomeric enzyme. Using isoelectric focusing, we determined a pI of 6.4 for the enzyme. The enzyme activity was greatest between pH5.5 and 6.0. The enzyme catalyzed the conversion of cannabigerolic acid or cannabinerolic acid to DELTA^1-tetrahydrocannabinolic acid. The V_<max> values were 2.68 and 0.37 nkat/mg for cannabigerolic acid and cannabinerolic acid, respectively. These results indicated that DELTA^1-tetrahydrocannabinolic acid is predominantly formed from cannabigerolic acid.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] FutoSHI TAURA: "A New Cannabinoids,Cannabinerlic Acid,from Cannabis Sativa" Phytochemistry. (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Futoshi Taura et al.: "New cannabinoid, cannabinerolic acid" from Cannabis sativa Phytochemistry. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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