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1994 Fiscal Year Final Research Report Summary

Gene analysis and gene therapy in tyrosinemias.

Research Project

Project/Area Number 05671886
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human genetics
Research InstitutionKumamoto University

Principal Investigator

ENDO Fumio  Department of Hospital Lecturer, 医学部・附属病院, 講師 (00176801)

Project Period (FY) 1993 – 1994
Keywordstyrosinemia / gene mutation / gene structure / animal model / recombinant adenovirus / gene therapy
Research Abstract

We have clone cDNA for human, mouse and porcine 4-hydroxyphenylpyruvate dioxygenase from cDNA libralies constructed from mRNA of human, mouse and porcine liver respectively, Using human and mouse cDNA as proves, we obstained DNA fragments containing exons of HPD from human and mouse genomic libraries, Through these studies we have clarified the structure of human and mouse HPD gene. Similarly, we have elucidated the structures of cDNA and gene for human fumarylacetoacetase.
Based on these progress we analyzed gene mutations in human FAH gene from a Japanese patient with type 1 tyrosinemia and mouse HPD gene from III mouse, a tyrosinemic mouse.These studies revealed mutations on FAH gene and HPD gene, respectively.
Using abino lethal mouse which is defective for Fah, and III mouse which is defective for HPD,we generated mice which carry both mutation, one on FAH gene and one on HPD gene. Fah deficient mice were neonatally lethal but these double mutants were vaiable, indicating that defective HPD gene rescues the lethal phenotype of albino deletion mouse.
Futher, we constructed recombinant adenovirus which express human HPD.In cultured cells, transduction of the recombinant virus effectively expresses human HPD.This recombinant virus will be useful to express human HPD in experimental animals.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Endo F.,et al.: "Measurement of blood holoceruloplasmin by El α using a mouse monoclonal antibody directed to holoceruloplasmin.Implicatin for mass screening of Wilson disease." J.Inher.Metab.Dis.17. 616-620 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo F.,et al.: "Structure of he human 4-hydroxyphenylpyruvic acid dioxygenase(HPD)" Genomics.23. 534-539 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo F.,et al.: "Characterization of a point mutation in the pyruvate El α gene from two boys with primary lactic acidemia." J.Inher.Metab.Dis.17. 189-195 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo F.,et al.: "Structtural organization and analysis of human fumarylacetoacetate hydrolase gene in tyrosinemia type I." Biochem,Biophys.Acta.1226. 168-172 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo F.,et al.: "A nonsense mutation in the 4-hydroxyphenylpyuvic acid dioxugenase causes skipping of the constitutive exon and hypertyrosinemia in mouse strain III." Genomics.25. 164-169 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo F.,et al.: "Exess copper and ceruloplasmin biosynthesis in long-term cultured hepatocytes from Long-Evans Cinnamon(LEC)rats,a model of Wilson disease." J.Biol.Chem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo F., et al.: "Measurement of blood holoceruloplasmin by E_1 alpha using a mouse monoclonal antibody directed to holoceruloplasmin. Implication for mass screening of Wilson disease." J.Inher.Metab.Dis.17. 616-620 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo F., et al.: "Structure of he human 4-hydroxyphenylpyruvic acid dioxygenase (HPD)" Genomics. 23. 534-539 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo F., et al.: "Chracterization of a point mutation in the pyruvate E_1alpha gene from two boys with primary lactic acidemia." J.Inher.Metab.Dis.17. 189-195 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo F., et al.: "Structural organization and analysis of human fumarylacetoacetate hydrolase gene in tyrosinemia type 1." Biochem.Biophys.Acta.1226. 168-172 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo F., et al.: "A nonsense mutation in the 40hydroxyphenylpyuvic acid dioxugenase causes skipping of the constitutive exon and hypertyrosinemia in mouse strain III" Genomics.25. 164-169 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo F., et al.: "Exess copper and ceruloplasmin biosynthesis in long-term cultured hepatocytes from Long-Ecans Cinnamon (LEC) ras, a model of Wilson disease" J.Biol.Chem. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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