• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1994 Fiscal Year Final Research Report Summary

INVOLVEMENT OF AN ACTIVATED POLYMORPHONUCLEAR IN CARDIOVASCULAR DISEASES

Research Project

Project/Area Number 05671906
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionShowa University

Principal Investigator

YAMAMOTO Toshinori  School of Pharmaceutical Sciences, Showa University, Associate Professor, 薬学部, 助教授 (30112741)

Co-Investigator(Kenkyū-buntansha) YASUDA Masako  School of Pharmaceutical Sciences, Showa University, Assistant Researcher, 薬学部, 助手 (30260079)
FUNAYAMA Norio  School of Pharmaceutical Sciences, Showa University, Assistant Researcher, 薬学部, 助手 (50245875)
SHIMIZU Shunichi  School of Pharmaceutical Sciences, Showa University, Assistant Researcher, 薬学部, 助手 (60196516)
Project Period (FY) 1993 – 1994
KeywordsENDOTHELIAL CELL / REACTIVE OXYGEN SPECIES / NITRIC OXIDE / POLYMORPHONUCLEAR / ISCHEMIA-REPERFUSION / HYDROGEN PEROXIDE / MUSCLE RELAXATION / CELL INJURY
Research Abstract

Early step of mechanisms on endothelial cells injury caused by an activated polymorphonuclear (PMN) are not well understood. Especially, the interactions of nitric oxide and active oxygen species (ROS) are not known. In this study, we focused on the endothelial function (muscle relaxing action) to clarify the contribution of extra-and intracellular nitric oxide and ROS to cell injury.
1) By the addition of activated PMN to blood vessel, at first the relaxation of blood muscle was a little reduced and then recovered gradually. This phenomena could be caused by the interaction of nitric oxide and ROS formed from PMN.
2) Hydrogen peroxide (1 mM) stimulated a formation of nitric oxide in endothelial cells. Enhacement of NO formation lasted until cell death.
3) During ischemia-reperfusion, the relaxing ability of blood vessel reduced at the moment and then return to the basal level following the reperfusion.
From these results, the tonus of blood vessel could be regulated by the interaction of NO and ROS.In endothelial cells, the NO formation increased by exposing to ROS.Reperfusion after short term ischemia caused a temporal inhibition of relaxing action. Finally, the early step of ROS injury are static and reversible.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 清水俊一: "Stimulation by hydrogen peroxide of L-arginine metabolism to L-citrulline coupled with nitric oxide synthesis in cultured endothelial cells." Res.Commun.Chem.Pathol.Pharmacol.84. 315-329 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水俊一: "Bradykinin induces generation of reactive species in bovine aortic endothelial cells." Res.Commun.Chem.Pathol.Pharmacol.84. 301-314 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Shimizu, Y.Saitoh, T.Yamamoto and K.Momose: "Stimulation by hydrogen peroxide of L-arginine metabolism to L-citrulline coupled with nitric oxide synthesis in cultured endothelial cells." Res.Commun.Chem.Pathol.Pharmacol.84. 315-329 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Shimizu, M.Ishii, T.Yamamoto, T.Kawanishi, K.Momose and Y.Kuroiwa: "Bradykinin induces generation of reacive oxygen species in bovine aortic endothelial cells." Res.Commun.Chem.Pathol.Pharmacol.84. 301-314 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1996-04-15  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi