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1994 Fiscal Year Final Research Report Summary

Expression of Recombination Activating Gene 1 (RAG-1) in Immature Hematopoietic Neoplasms

Research Project

Project/Area Number 05671925
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionKobe University

Principal Investigator

TATSUMI Eiji  Kobe University School of Medicine Associate Professor, 医学部, 助教授 (20192172)

Project Period (FY) 1993 – 1994
KeywordsRAG-1 (Recombination Activating Gene-1) / Undifferentiated Hematopoietic Neoplasms / TdT (Terminal Deoxy-nucleotidyl Transferase / Lymphoid Neoplasms / Thymic Stage / Pro-thymic Stage
Research Abstract

The detection of monoclonal rearrangements of the genes of immunoglobulins (Ig) or T-cell receptors (TCR) has recently become popular in the studies of hematopoietic neoplasms. It is useful for proving monoclonality. However, it has also been shown that the derived lineage or stage of differentiation in a given hematopoietic neoplasm can be clarified only very rarely by the gene rearrangement analysis of Ig or TCR,when other types of studies including phenotypic analysis fail to reveal the lineage- or stage-derivation. Furthermore, such gene analysis can detect only the resultant products of the rearrangement, and is little informative regarding the question if the cells can rearrange the Ig or TCR genes. Thus, the expression of RAG-1 (Recombination Activating Gene-1) , a recombinase itself or a molecule closely related to the recombinase, was investigated in cell-line or fresh human neoplastic cells.
First, the expression of RAG-1 was investigated in 31 human hematopoietic cell-lines. … More RAG-1 was detected in immature lymphoid cells, but not in mature lymphoid or Hodgkin cells, proving the feasibility of the assay. Then, the investigation was performed in 45 fresh cases. In B-lineage, RAG-1 was high in CD19+10-20- or CD19+10+20-, but low in CD19+10+20+ stage. In T-lineage, the RAG-1 expression was absent or limited in the pro-thymic stage (CD7+5-2-, CD7+5+2- or CD7+5+2+3-4-8-) , intense in the thymic stage (CD3<plus-minus>4+8+) and modest in the late thymic stage(CD3+4+8-). Generally, TCRdelta/gamma gene is rearanged in the pro-thymic stage, but TCRbeta gene not. Two conclusive findings are : [1] The expression of RAG-1 is preserved after neoplastic transformation, indicating the usefullness of the hematopoietic neoplasms for delineating the differentiation scheme of normal hematopiesis. [2] The contribution of RAG-1 to the gene rearrangement of TCRdelta/gamma is at least limited compared with that to the gene rearangement of TCRbet
The latter finding is particularly important, requiring further investigations, since the results of the RAG-1 gene-knock-out studies in mouse are interpreted to indicate that RAG-1 is indispensable for rarranging TCRdelta/gamma gene as well as TCRbeta gene. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yoneda N: "Recombination activation gere-1(RAG-1)in lcnkemia/lymphoma cells. Expression depends in Stage of differentiation defined by phcnotype and genjtype" Blood. 82. 207-216 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 朱田 現子: "PCR法によるRAG-1遺伝子の検索" 医学のあゆみ. 157. 709-710 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano S: "Suppression of gene expression of myeloper oxiduse (mPO) by IFN-γ" Cymphokine and Cytokine Res. 12. 81-85 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano S: "Pattem of expression of CD45 RA/RO isoformic antigens in acute myeloblastic leukemia cells" Am J Clin Patho 1. 100. 386-393 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsumi E: "CD21 antigen in T-lineage Neoplastic Lymphoid Cellsi Chavacteristic Expression at Thymic Stage" Am J Hemato 1. 45. 150-155 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoneda N: "Lineage determination of CD7+CDS-CD2-and CD7+CD5+CD2-lymphoblasto: Studieo an phenotype, genotype and gene expression of myeloperoxiduse, CD3ε and CD38" Am J Hematol. 45. 310-320 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoneda N,Tatsumi E,Kawano S,Matsuo Y,Minowada J,Yamaguchi N: "Recombination Activating Gene-1 (RAG-1) in Leukemia/Lymphoma Cells : Expression Depends on Stage of Lymphoid Differentiation Defined by Phenotype and Genotype." Blood. 82. 207-216 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S,Tatsumi E,Yoneda N,Nagata S,Yamaguchi N: "Suppression of Gene Expression of Myeloperoxidase (MPO) by Gammam Interferon (IFN-gamma) in HL60 cells." Lymphokine and Cytokine Res. 12. 81-85 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S,Tatsumi E: "CD45 RA/RO Isoformic Antigens in Acute Myeloblastic (AML) Leukemia Cells." Am J Clin Pathol. 100. 386-393 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsumi E,Yoneda N,Kawano S,Yamaguchi N: "CD21 Antigen in T-lineage Neoplastic Lymphoid Cells : Characteristic Expression at Thymic Stage." Am J Hematol. 45. 150-155 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoneda N,Tatsumi E,Teshigawara K,Nagata S,Nagano T,Kishimoto Y,Kimura T,Yasunaga K,Yamaguchi N: "Lineage Lineage determination of CD7+ CD5- CD2- and CD7+ CD5+ CD2- lymphoblasts : Studies on phenotype, geneotype and gene expression of myeloperoxidase (MPO) , CD3epsilon and CD3delta dhains." Am J Hematol. 45. 310-320 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S,Tatsumi E,Yoneda N,Yamaguchi N: "Expression Pattern of CD45 RA/RO Isoformic Antigens in T-lineage Neoplasms." Am J Hematol. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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