1994 Fiscal Year Final Research Report Summary
Investigation of Steroid Sulfate Conjugates in Neonatal Serum-Elucidation of the Mechanism of the Biosynthesis of the Steroids and Its Application for Pathological Analysis.
| Project/Area Number |
05671935
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
WATANABE Fukuko Kobe Pharmaceutical University, Faculty of Pharmacy, Professor, 薬学部, 教授 (10068322)
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| Co-Investigator(Kenkyū-buntansha) |
SUMINOE Akemi Kobe Pharmaceutical University, Faculty of Pharmacy, Instructor, 薬学部, 助手 (40258124)
TAGAWA Noriko Kobe Pharmaceutical University, Faculty of Pharmacy, Instructor, 薬学部, 助手 (00206905)
KOBAYASHI Yoshiharu Kobe Pharmaceutical University, Faculty of Pharmacy, Associate Professor, 薬学部, 助教授 (20133647)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Adrenal steroid / Sulfate conjugate / 16-Dehydropregnenolone 3-sulfate |
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| Research Abstract |
The present study was designed to investigate the conjugation form of 16-dehydropregnenolone (16-DHP) which had been identified in the serum of neonates in our laboratory and its metabolic site.
Free steroids were washed out from neonatal serum by CH_2Cl_2 treatment. The residue which may contain conjugated steroids was extracted with MeOH.The MeOH extracts were applied to HPLC.The fraction, in which authentic 16-DHP 3-sulfate was eluted, was collected. This fraction was treated with arylsulfatase. Liberated free steroids was analyzed by GC/MS and GC/FT-IR.All of the data obtained was identical with those of authentic 16-DHP.These results showed that 16-DHP is conjugated with sulfuric acid.
To investigate if 16-DHP 3-sulfate is synthesized in fetal adrenal, correlation of serum levels of 16-DHP 3-sulfate and gestational age was observed. As a result, significant negative correlation was observed between these two parameters. These results suggest that 16-DHP 3-sulfate is synthesized in fetal adrenal.
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