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1994 Fiscal Year Final Research Report Summary

Probing DNA Structure by Photoelectron Transter and Its Application toward Gene The rapy

Research Project

Project/Area Number 05680524
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SUGIYAMA Hiroshi  KYOTO UNIVERITY,ASSOCIATE FACULTY OF ENGINEERING,PROFESSOR, 工学部, 助教授 (50183843)

Co-Investigator(Kenkyū-buntansha) SAITO Isao  KYOTO UNIVERSITY,FACULTY OF ENGINEERING,PROFESSOR, 工学部, 教授 (20026082)
Project Period (FY) 1993 – 1994
Keywordsphoto electron transfer / DNA local conformatiom / DNA strand scission
Research Abstract

DNA conformation plays an important role in nearly every aspect of DNA function, including DNA replication, gene expression and regulation. Understanding DNA conformation and its role in biological processes is intense current interest and it will be a valuable information in designing new anticancer and gene-thepeutic agents. The aim of this research is to evaluate the potential of 5-halouracil photoreaction as DNA local conformational analysis and conformation-specific DNA cleaving agent. It was found that photoiradiation of bromouracil (BrU) -containing oligonucleotide efficiently produces deoxyribose 1' oxidation product at adenine residue of ABrU Sequence, while iodoracil (IU) -containing oligomer produces comparable amount of 1' and 2' oxidation products. Detailed mechanism of oxidative DNA strand scission including 1,2-hydride shift in the formation of 1' oxidation product was elucidated. ABrU photochemical unit was introduced into various type of DNA conformations. It was found that A conformation enhanced efficiency of the reaction and specificity of 1' oxidation was increased. Introduction of mismatch at adenine site does not change the photoreactivity whereas introduction of mismatch at BrU site greatly reduced photoreactivity.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 杉山弘: "Chemistry of Thermal Degration of Abasic Sites in DNA" Chem.Res.Toxicol. 7. 673-683 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉山弘: "Actionation of Neocargiotation-Chromophore by 4-Hychoxythiophenol." Tetrahedoron Letters. 35. 8825-8828 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉山弘: "Oligonuculeotide Containing a Radical Trap." J.Am.Chem.Soc.116. 10326-10327 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉山弘: "Stereospecific 1,2-Hydeide Shift in Ribonolactone Formetion in the Photoreaction of 2-Iododeoxyuridine" J.Am.Chem.Soc.117(印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Sugiyama: "Chemistry of Thermal Degration Abasic Sites in DNA" Chem.Res.Toxicol. 7. 673-683 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Sugiyama: "Actionation of Neocargiotation - Chromophore by 4-Hychoxythiophenol" Tetrahedoron Letters. 35. 8825-8828 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Sugiyama: "Oligonucleotide Containing a Radical Trap" J.Am.Chem.Soc.116. 10326-10327

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Sugiyama: "Stereospecific 1,2-Hydeide Shift in Ribonolactone Formetion in the Photoreaction of 2-Inododeoxyridine" J.Am.Chem.Soc.117 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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