1994 Fiscal Year Final Research Report Summary
Fission yeast genes which function during meiosis
| Project/Area Number |
05680588
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
WATANABE Yoshonori The University of Tokyo, Graduate School of Science Research, associate, 大学院・理学系研究科, 助手 (20212326)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Masayuki The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (40114706)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Keywords | meiosis / RNA / RNA recognition motif / premeiotic DNA synthesis / first meiotic division / fission yeast |
|---|
| Research Abstract |
The molecular controls over meiosis are poorly under-stood compared with those over mitosis. Here, we show that S.pombe mei2, which is essential for the initiation of premeiotic DNA synthesis, encodes an RNA-binding protein. A temperature-sensitive mei2 mutant performs premeiotic DNA synthesis but does not undergo meiotic divisions, suggesting thet Mei2 is required also for meiosis I.A novel, polyadenylated RNA species (meiRNA) , which suppresses this temper-ature-sensitive defect if overexpressed, specifically binds to Mei2 both in vivo and in vitro. Cells without meiRNA perform premeiotic DNA synthesis but cannot undergo meiosis I.Mutations that apparently block the RNA binding ability of Mei2 inhibit premeiotic DNA syn-thesis. Mei2 is thus likely to couple with another RNA species to promote premeiotic DNA synthesis.
|
Research Products
(6 results)
