• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1994 Fiscal Year Final Research Report Summary

Studies on the mechanism of nuclear-cytoplasmic transport -From the studies of the influenza virus assembly

Research Project

Project/Area Number 05680589
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionKanazawa University

Principal Investigator

FIKUDA Ryuji  Kanazawa Univ., School of Medicine, Professor, 医学部, 教授 (60027331)

Co-Investigator(Kenkyū-buntansha) TAKIZAWA Takenori  Kanazawa Univ., School of Medicine, Assistant Professor (joined in the project A, 医学部, 講師 (40192158)
HATADA Eriko  Kanazawa Univ., School of Medicine, Instructor, 医学部, 助手 (70228469)
Project Period (FY) 1993 – 1994
KeywordsNuclear-cytoplasmic Transport / Influenza Virus / Nuclear Pore / M1 Protein / NA Protein / Virus Assembly / Reverse Genetics / ウイルス粒子形成
Research Abstract

We have realized that the studies of the influenza virus growth provide several interesting problems concerning nuclear-cytoplasmic transport. In the early stage of virus assembly, the virus genomic RNA produced in the nucleus immediately with virus core proteins, and the ribonucleoprotein complex (vRNP) thus formed is transported through the nuclear pore into the cytosol, and then into budding virus particles on the plasma membrane. In the first part of this study, nuclear-cytoplasmic transport of vRNPs was studied by analysing the distribution of vRNAs employing an in situ hybridization technique. The analyzes were performed using wild-type virus as well as a ts mutant virus, ts-51, which harbors a mutation in the segment 7, and has a defect in the late phase of virus growth. Nucleotide sequence analysis revealed a single amino acid change in the M1 protein. In the ts-51 virus-infected cells at a nonpermissive temperature, more than 95% of the vRNAs and the M1 protein remained in the nucleus, even at 6 hrpi and thereafter, when about 50% of them moved to the cytoplasm for the wild-type virus.These observations indicated that the M1 protein participated in the nuclear-cytoplasmic transport of the vRNAs. One hypothesis was that the M1 was associated with vRNPs in the nucleus forming possible M1-vRNP complexes, which were then transported into the cytosol. In the second part of this study, the virus assembly process in plasma membrane was investigated on the significance of the conserved sequence of the NA protein (its cytoplasmic domain and a successive sequence of the transmembrane domain) by the reverse genetic technique. It was indicated that both successive regions playd important roles in the formation of the infective virus particles.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] K.Enami 他3名: "An influenza virus temperature-sensitive mutant defective in the nuclear-cytoplasmic transport of the negative-sense-viral RNAs" Virology. 194. 882-827 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takizawa 他5名: "Induction of programmed cell death(apoptosis)by influenza infection in tissue culture cells" J.General Virology. 74. 2347-2355 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takizawa 他4名: "Activation of the apoptotic Fas antigen-encoding gene upon influenza virus infection involuing spontaneously produced beta-interferon" Virology. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ohmori 他4名: "dinP,a new gene in Escherichia coli,whose product shows similarities to UmuC and its homolegues" Mutation Research Letters. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Enami, Y.Qiao, R.Fukuda, and M.Enami: "An influenza virus temperature-sensitive mutant defective in the nuclear-cytoplasmic transport of the negative-sense viral RNAs." Virology. 194. 822-827 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Takizawa, S.Matsukawa, Y.Higuchi, S.Nakamura, Y.Nakanishi and R.Fukida: "Induction of programd cell death (apoptosis) by influenza virus infection in tissue culture cells." J.Gen.Virol.74. 2347-2355 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Takizawa, R.Fukuda, T.Miyawaki, K.Ohashi and Y.Nakanishi: "Activation of the apoptotic Fas antigen-encoding gene upon influenza virus infection involving spontaneously produced bera-interferon" Virology. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Ohmori, E.Hatada, Y.Qiao, M.Tsuji and R.Fukuda: "dinP,a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues" Mutation Research Letters. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1996-04-15  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi