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1994 Fiscal Year Final Research Report Summary

Signal transduction for activation of cell cycle inducing factors during dedifferentiation of muscle cells

Research Project

Project/Area Number 05680604
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionChiba University

Principal Investigator

ENDO Takeshi  Chiba University Department of Biology, Faculty of Science Associate Professor, 理学部, 助教授 (30194038)

Project Period (FY) 1993 – 1994
KeywordsMuscle cell differentiation / Dedifferentiation / SV40 large T antigen / c-Jun / AP-1 / Rb / MyoD / Myogenin
Research Abstract

The mouse skeletal muscle cell line C2SVT has been established by transfecting the C2 cells with the SV40 large T antigen gene under the control of an inducible promoter. When the large T is induced in preformed C2SVT myotubes, the terminally differentiated quiescent cells reenter the cell cycle to undergo mitosis. During this dedifferentiation process, only c-Jun among various nuclear protooncogene products was coordinately induced with the expression of large T.The transcription factor AP-1 was also activated. The experiments with antisense olignucleotides showed that the induced c-Jun, possibly as a homodimer, is essential for the myotubes to resume the cell cycle. When large T was induced in both the myoblasts and myotubes, neither the amount of phosphotyrosine, the activity of phospholipase C,Ras, nor MAP kinase was elevated. Thus, large T,a nuclear protein, is likely to induce the cell cycle without activating these cytoplasmic signaling molecules. Further, bacterially expressed GST-MyoD and GST-myogenin were shown to bind to large T expressed in the Sf-9 cells through the baculovirus system. Under examination are domains involved in this interaction and whether or not the growth supperssion function of thses MyoD family proteins is prevented by the interaction.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] T.Endo: "SV40 large T inhibits myogenic differetiation partially through induching c-jun." J.Biochem.112. 321-329 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Endo: "Retionblastoma gene product Rb accumulates during myogenic differentiation and is deinduced by the expression of SV40 large T antigen." J.Biochem.112. 427-430 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Fukami: "α-Actinin and vinculin are PIP_2 binding proteins involved in signaling by tyrosine kinase." J.Biol,Chem.269. 1518-1522 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Sakurai: "Activin A stimulates mitogenesis in Swiss 3T3 fibroblasts without activation of mitogen-activated protein kinases." J.Biol,Chem.269. 14118-14122 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohkubo: "SV40 large T antigen reinduces the cell cycle in terminally defferentiated myotubes through inducing Cdk2,Cdc2,and their partner cyclins." Exp.Cell Res.214. 270-278 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Hashimoto: "Phosphorylation of a proline-directed kinase motif is responsible for structural changes in myogenin." FEBS Lett.352. 236-242 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永田和宏ら 編: "分子生物学・免疫学キーワード辞典" 医学書院, 530 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo, T.: "SV40 large T inhibits myogenic differentiation partially through inducing c-jun." J.Biochem.112. 321-329 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo, T.: "Retinoblastoma gene product Rb accumulates during myogenic differentiation and is deinduced by expression of SV40 large T antigen." J.Biochem.112. 427-430 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakae, H.: "Ascidian entactin/nidogen : implication of evolution by shuffling two kinds of cysteine-rich motifs." Eur.J.Biochem.213. 11-19 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maruyama, K.: "A novel domain sequence of connectin localized at the I band of skeletal muscle sarcomeres : homology to neurofilament subunits." Biochem.Biophys.Res.Commun.194. 1288-1291 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maruyama, K.: "A partial connectin cDNA encoding a novel type of RSP motifs isolated from chicken embryonic skeletal muscle." J.Biochem.115. 147-149 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Fukami: "alpha-Actinin and vinculin are PIP_2 binding proteins involved in signaling by tyrosine kinase." J.Biol.Chem.269. 1518-1522 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Sakurai: "Activin A stimulates mitogenesis in Swiss 3T3 fibroblasts without activation of mitogen-activated protein kinases." J.Biol.Chem.269. 14118-14122 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ohkubo: "SV40 large T antigen reinduces the cell cycle in terminally differentiated myotubes through inducing Cdk2, Cdc2, and their partner cyclins." Exp.Cell Res.214. 270-278 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Hashimoto: "Phosphorylation of a proline-directed kinase motif is responsible for structural changes in myogenin." FEBS Lett.352. 236-242 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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