1994 Fiscal Year Final Research Report Summary
Identification and functional analysis of cytoplasmic factors involved in nuclear protein transport
| Project/Area Number |
05680612
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
IMAMOTO Naoko Osaka University Medical School, Department of Anatomy and Cell Biology, Assistant Professor, 医学部, 助手 (20202145)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Keywords | nulclear transport / nuclear location signal / nuclear pore / in vitro transport system |
|---|
| Research Abstract |
Targeting of karyophilic proteins to nuclear pores is known to require several cytoplasmic factors including the nuclear location signal (NLS) -binding protein. Using a digitonin-permeabilized cell-free transport assay, we have obtained a cytoplasmic fraction containing factors that specifically bind to karyophilic protein and support the nuclear binding step of the transport. Components in this fraction form a stable complex with the karyophile through interaction with NLS.Since this complex shows nuclear pore binding activity prior to nuclear entry in the absence of other cytosolic factors, we call it nuclear pore-targeting complex. It consists of karyophilic protein and four proteins of 54,56,66, and 90kDa. In our reconstitution experiments, a complex with 54 and 90kDa proteins is capable of targeting karyophiles to the nuclear pores.
|
