1994 Fiscal Year Final Research Report Summary
Plasma membrane-Actin filament Association through ERM Family Members
| Project/Area Number |
05680627
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | KYOTO UNIVERSITY (1994)
Okazaki National Research Institutes (1993) |
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Principal Investigator |
TSUKITA Sachiko Kyoto University, College of Medical Technology, Prof., 医療技術短期大学部, 教授 (00188517)
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| Project Period (FY) |
1993 – 1994
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| Keywords | CD44 / actin filaments / ERM / radixin / ezrin / moesin / plasma membrane |
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| Research Abstract |
The ERM family members, ezrin, radixin, and moesin, localizing just beneath the plasma membranes are thought to be involved in the actin filament/plasma membrane association. To identify the integral membrane protein directly associated with ERM family members, we performed immunoPrecipitaion studies using anti-moesin mAb and cultured BHK cells metabol-ically-labeled with [^<35>S] methionine or surface-labeled with biotin. The results indicated that moesin is directly associated with a 140kD integral membrane protein. Using BHK cells as antigens, we obtained a mAb which recognized the 140kD membrane protein. We next cloned a cDNA encoding the 140kD membrane protein and identified it as CD44, a broadly-distributed cell surface glycoprotein. Immunoprecipitation with various anti-CD44 mAbs showed that ezrin and radixin as well as moesin are associated with CD44 not only in BHK cells but also in mouse L fibroblasts. Furthermore, immunofluorescence microscopy revealed that in both BHK and L cells, the Triton X-100-insoluble CD44 is precisely colocalized with ERM family members. We concluded that ERM family members work as molecular linkers between the cytoplasmic domain of CD44 and actin-based cytoskeletons.
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[Publications] Itoh, M., Nagafuchi, A., Yonemura, S., Yasuda-Kitani, T., Tsukita, Sa., and Tsukita, Sh.: "The 220kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells : cDNA cloning and immunoelectrom microscopy" J.Cell Biol.121. 491-502 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hashimoto, T., Amagai, M,Parry, D.A.D., Dixon, T.W., Tsukita, Sa., Tsukita, Sh., Miki, K., Sakai, K., Inokuchi, Y., Kudoh, J., Shimizu, N., and Nishikawa, T.: "Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK." J.Cell Sci.105. 275-286 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Furuse, M., Hirase, T., Itoh, M., Nagafuchi, A., Yonemura, S., Tsukita, Sa., and Tsukita, Sh.: "Occludin : A novel integral membrane protein localizing at tight junctions" J.Cell Biol.123. 1777-1788 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Kishino, T., Ariga, T., Soejima, H., Tamura, T., Ohta, T., Jinno, Y., Yonemura, S.Sato, N., Tsukita, Sa., Tsukita, Sh., Sakiyama, Y., and Niikawa, N.: "Assignment of the human moesin gene (MSN) to chromosome region Xq11.2-q12" Cytogenet.Cell Genet.66. 167-169 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Takeuchi, K., Sato, N., Kasahara, H., Funayama, N., Nagafuchi, A., Yonemura, S., Tsukita, Sa., and Tsukita, Sh.: "Perturbation of cell adhesion and microvilli formation by antisense oligonuclotides to ERM family members." J.Cell Biol.125. 1371-1384 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Tsukita, Sa., Oishi, K., Sato, N., Sagara, J., Kawai, A., and Tsukita, Sh.: "ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons." J.Cell Biol.126. 391-401 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Furuse, M., Itoh, M., Hirase, T., Nagafuchi, A., Yonemura, S., Tsukita, Sa., and Tsukita, Sh.: "Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions." J.Cell Biol.127. 1617-1626 (1994)
Description
「研究成果報告書概要(欧文)」より
