1995 Fiscal Year Final Research Report Summary
Role of zebrafish POU-domain gene, ZFPOU2, in early development
| Project/Area Number |
05680648
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Nagoya University (1994-1995)
The Institute of Physical and Chemical Research (1993) |
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Principal Investigator |
TAKEDA Hiroyuki Nagoya University, School of Science Associate Professor, 理学部, 助教授 (80179647)
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| Project Period (FY) |
1993 – 1995
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| Keywords | POU transcription factor / Zebrafish / Gastrulation / Mesoderm induction / ES cell |
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| Research Abstract |
POU-domain proteins are a large family of transcriptional regulatory proteins, many of which are implicated in the control of gene expression during early development. We describe here the cloning and expression of zebrafish pou2, a novel POU-domain gene related to the mouse germ line-specific transcription factor Oct-3. Zebrafich pou2 is maternally expressed and the transcripts are present from the one-cell stage to the gastruls stage. In situ hybridization analyzes revealed that the transcripts were present in all blastomeres until the midblastula stage and that the expression was restricted to the epiblast during gastrulation. We found that alternatively spliced transcripts, t-pou2 RNA,were also expressed in the embryos. In contrast to the Pou2 product, t-Pou2 product lacks DNA-binding activity because of its incomplete POU-domain structure. To examine the roles of the Pou2 and t-Pou2 products, we increased their expression in the embryo by microinjection of synthetic pou2 and t-pou
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2 RNAs into the fertilized eggs at the one-cell stage. Most embryos that developed from the eggs injected with pou2 RNA did not show any obvious developmental defects. In contrast, overexpression of the t-Pou2 product greatly affected the embryonic development : there was strong developmental retardation or arrest due to the incomplete gastrulation. In the affected embryos, expression of zebrafish T gene was reduced and the hypoblast formation was disturbed. Temporal and spatial expression patterns and the effects of overxpression of these products on development are consistent with the idea that the Pou2 and t-Pou2 proteins are involved in early development of zebrafish embryos. They may be involved in the proliferation of blastomeres in undetermined state at the blastula stage and/or the early cell commitment events at the gastrula stage. Also, our results indicate that different products generated as a result of alternative splicing from the same gene possess distinct functional capacities. Less
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