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1994 Fiscal Year Final Research Report Summary

Roles of G-proteins and cytoskeletal porteins in transmitter release.

Research Project

Project/Area Number 05680683
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionSophia University

Principal Investigator

KUMAKURA Konosuke  Sohia University, Faculty of Science and Technology, Professor, 理工学部, 教授 (70129790)

Co-Investigator(Kenkyū-buntansha) IMAIZUMI Mica  Sophia University, Life Science Institute, Assistant, 生命科学研究所, 助手 (40201941)
Project Period (FY) 1993 – 1994
KeywordsMechanism of Exocytosis / Transmitter Release / GTP-Binding Proteins / Myosin Light Chain Kinase / Ca^<2+>-Dependent Release / ATP-Dependent Priming / Mastoparan / Cytoskeletal Proteins
Research Abstract

The aim of this research project is to clarify the roles of GTP-binding proteins and cytoskeletal proteins in the mechanism of transmitter release.Previously we have suggested that Go, one of heterotrimeric GTP-binding proteins, negatively regulates Ca^<2+>-dependent release of catecholamine in permeabilized chromaffin cells.We also have suggested a possible involvement of myosin light chain kinase in the mechanism of Ca^<2+>-dependent exocytosis.In the project, we attempted to elucidate the mechanism whereby Ca^<2+>-dependent release is regulated by Go, and by myosin light chain kinase in permeabilized chromaffin cells.We have obtained the following results.
1.By use of mastoparan, a peptide known to activate directly GTP-binding proteins, and by use of anti-Go antibodies, we found that the negative regulation by Go is on the process of ATP-dependent priming of exocytosis.This inhibitory Go was suggested to be activated by GAP43, and activation of protein kinase C seemed to stimulate Ca^<2+>-dependent release by abolishing the action of GAP43 on Go.
2.We found that myosin light chain kinase is essential for ATP-dependent priming of exocytosis, by use of specific inhibitors of myosin light chain kinase, wortmannin and SM-1.Histochemical studies suggested that the inhibition of myosin light chain kinase inhibits reorganization of cortical F-actin, and thus suggested an important role of cortical F-actin for priming of chromaffin vesicles to the exocytotic site.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 笹木 君仁: "開口放出のツ-ステップ・モデルにおけるミオシン軽鎖キナーゼの役割" 神経化学. 32. 122-123 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 熊倉 鴻之助: "開口放出の調節機構-クロマフィン細胞を中心にして-" 生体の科学. 44. 215-223 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今泉 美佳: "開口放出と3量体Gタンパク質" 細胞工学. 13. 373-380 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 御園生 裕明: "開口放出のPriming Stepに対するMastoparanの抑制作用" 神経化学. 33. 150-151 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konosuke Kumakura: "Essential Role of Myosin Light Chain Kinase in the Mechanism for MgATP-Dependent Priming of Exocytosis in Adrenal Chromaffin Cells" Journal of Neuroscience. 14. 7695-7703 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki, Kimihito: "Implication of myosin light chain kinase in the mechanism of exocytosis in adrenalchromaffin cells." Bulletin of the Japanese Neurochemical Society. 32. 122-123 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumakura, Konosuke: "Regulation of exocytosis in adrenal chromafin cells." Seitai no Kagaku. 44. 215-223 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imaizumi, Mica: "Roles of heterotrimeric GTP-binding proteins in exocytosis." Cell Technology. 13. 37-44 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Misonou, Hiroaki: "Inhibition of ATP-dependent priming of exocytosis by mastoparan." Bulletin of the Japanese Neurochemical Society. 33. 150-151 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumakura, Konosuke: "Essential role of myosin light chain kinase in the mechanism for ATP-dependent priminbg of exocytosis in adrenal chromaffin cells." Journal of Neuroscience. 14. 7695-7703 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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