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1995 Fiscal Year Final Research Report Summary

Search for Transglutaminase Activity Change accompanying with Synaptic Transmission Process

Research Project

Project/Area Number 05680685
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionDepartment of Physiology, School of Medicine, Fujita Health University

Principal Investigator

NAGATA Yutaka  Department of Physiology, School of Med., Fujita Health Univ.Prof., 医学部, 教授 (70084499)

Co-Investigator(Kenkyū-buntansha) YAMAUCHI Masamitsu  Dept of Physiol., School of Med., Fujita Health Univ.Instructor, 医学部, 助手 (30278303)
ANDO Masato  Dept of Physiology, School of Med., Fujita Health Univ.Instructor, 助教授 (40097720)
Project Period (FY) 1993 – 1995
Keywordssynaptic transmission / transglutaminase / neurotransmitter (carbachol) / nitric oxide / cyclic GMP
Research Abstract

Superior cervical sympathtic ganglion (SCG) contains many cholinergic synapses, and maintains impulse transmission activity after removal from the rat. When neurotransmitter (acetylcholine ; Ach) was applied to the physiological saline medium during aerobic incubation of the excised SCG,rapid and significant rise in activity of Ca^<2+> -dependent eNzyme, transglutaminase (TG) was observed. Carbachol, a muscarinic ACh agonist, strongly increased the tissue TG activity within minutes, which was fairly antagonized by a muscarinic ACh antagonist, atropine. Nitric oxide (NO), a new transmitter candidate, remarkably accelerated TG activity in the SCG,and the activity was suppressed by a specific inhibitor for NO synthase, N^G -monomethyl-L-arginine. The rate of NO activation was measured byh the formation of cyclic GMP,but the an inhibitor of guanylate cyclase, methylene blue, did not supressed the Carb-induced TG activity increase. These results suggest that Carb -stimulation to the SCG neurons initiate the intracellular signal transfer process for the activation of TG via receptor mediated NO and cyclic GMP increase to cause intracellular Ca^<2+> rise.

  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Ando, Nagata et al.: "Selective alteration in transglutaminase activity..." Brain Research. 604. 64-68 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando and Nagata: "Effect of depolarizing agents on transglutaminase..." Molecular and Chemical Neuropathology. 19. 121-135 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando, Nagata et al.: "Rapid and transient alteration in transglutaminase..." Neuroscience Research. 17. 47-52 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤,永田(他): "神経成長因子による摘出白鼠交感神経節内トランスグルタミナーゼ・・" 神経化学. 32. 394-395 (1993)

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      「研究成果報告書概要(和文)」より
  • [Publications] 安藤,永田(他): "アセチルコリン(ACh)あるいはノルエピネフリン(NE)神経伝達物質・・" 日本生理学雑誌. 55. 25-26 (1993)

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      「研究成果報告書概要(和文)」より
  • [Publications] 安藤,永田(他): "神経成長因子による白鼠交感神経節内トランスグルタミナーゼ活性" 藤田学園医学会誌. 16. 87-93 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando, Nagata et al.: "Effect of axotomy on nitric oxide-dependent cyclic..." Neuroscience Research. 19. 67-72 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando, Nagata et al.: "Blockade effect of nerve growth factor on GM1 ganglio-..." Neuroscience Research. 19. 373-378 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando, Nagata et al.: "The intercellular communication via nitric oxide..." Brain Research. 650. 279-285 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、永田(他): "白鼡上頸部交感神経節内一酸化窒素依存性環状GMP生成系・・・" 藤田学園医学会誌. 17. 373-378 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、永田(他): "カルバコール刺激に依存した白鼡上頸部交感神経節内一酸化窒素.." 藤田学園医学会誌. 18. 81-88 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、永田(他): "カルバコール刺激による白鼡上頸部交感神経節トランスグルタミ." 藤田学園医学会誌. 18. 105-112 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、永田(他): "白鼡上頸部交感神経節内一酸化窒素依存性環状GMP生成系・・・・" 神経化学. 33. 512-513 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、永田(他): "カルバコール刺激依存性の白鼡上頸部交感神経節内トランスグル.." 神経化学. 33. 564-565 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando, Nagata et al.: "Possible involvement of nitric oxide in carbachol-..." Neuroscience Research. 21. 267-272 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, Nagata et al: "Alteration of transglutaminase activity in rat and ..." Neuroscience Research. 20. 1195-1201 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、山内、永田(他): "Carbachol刺激による白鼠上頸部交感神経節内superoxide dismutase・・" 藤田学園医学会誌. 19(1). 101-106 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、山内、永田(他): "グルタミン酸毒性によるラット脊髄組織変性過程におよぼす核酸および・・" 藤田学園医学会誌. 19(2). 203-211 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、藤田、永田(他): "筋萎縮性側索硬化症患者脊髄中のSuperoxide DismutaseおよびCyto・・" 藤田学園医学会誌. 19(2). 258-263 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤、藤田、永田(他): "カルバコール刺激による白鼠上頸部神経節内トランスグルタミナーゼ・・" 神経化学. 24. 130-131 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤田、安藤、永田(他): "筋萎縮性側索硬化症(ALS)患者脊髄から得られた特異タンパク質の解析" 神経化学. 34. 158-159 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田丸、松谷、永田(他): "実験的小頭症ラット線状体のドーパミン及びその代謝物の変化:in vivo・" 神経化学. 34. 194-195 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Ando, Yamaguchi, Nagata: "Induction of tissue transglutaminase in rat superior..." Neuroscience Research. 21(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, Yamaguchi, Nagata: "Remarkable reduction of transglutaminase activity in .." Cellular and Molecular Neurobiology. 16(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando and Nagata: "Effect of depolarizing agents on transglutaminase activity, Ca^<2+>-influx, -" Molecular and Chemical Neuropathology. 19. 121-135 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, Nagata et al.: "Selective alteration in trans glutaminase activity of rat superior cervical-" Brain Research. 604. 64-68 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, Nagata et al.: "Rapid and transient alteration transglutaminase activity in rat superior-" Neuroscience Research. 17. 47-52 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, Nagata et al.: "Effect of axotomy on nitric oxide-dependent cyclic GMP production of rat-" Neuroscience Research. 19. 67-72 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, Nagata et al.: "Blockade effect of nerve growth factor on GM1 ganglioside-induced activation-" Neuroscience Research. 19. 373-378 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, Nagata et al.: "The intercellular communication via nitric oxide and its regulation in coupling-" Brain Research. 650. 279-285 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, Nagata et al.: "Possible involvement of nitric oxide in carbachol-induced activaiton of-" Neuroscience Research. 21. 267-272 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita, Nagata et al.: "Alteration of transglutaminase activity in rat and human spinal cord-" Neuroscience Research. 20. 1195-1201 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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