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1994 Fiscal Year Final Research Report Summary

Analysis and indentification of proteins related to self-incompatibility types in tree crops

Research Project

Project/Area Number 05806001
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

IKEHASHI Hiroshi  Kyoto Univ.Fac.Agriculture, Professor, 農学部, 教授 (50193222)

Project Period (FY) 1993 – 1994
Keywordsself-imcompatibility / Japanese pear / Apple / RNase / Glyco-protein / Breeding
Research Abstract

Using several varieties of Japanese pear (Pyrus serotina Rehd.) , each of which has known genotype in regards to self-incompatibility, proteins extracted from the stylars were analyzed by two dimensional gel elecctro-phoresis. As the results, self-incompatibility-related RNases which cosegregate with S-genotypes were identified. The RNases were dificient in a self-compatible mutant of Japanese pear. N-terminal aminoacid sequences of the RNases showed clear homology with S-RNases of Petunia.
Applying the above results, self-incompatibility-related glycoproteins in styles of apple (Malus x domestica) were identified. The self-incompatibility genotypes for more than ten varieties were determined.
Self-incompatibility-related RNases in Pyrus communis were studied. Loss of one of S-RNases was confirmed in self-compatible cultivars.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Sassa, H.: "Identification and characterization of stylar glycoproteins associtated with self-incompatibility genes of Japabese pear, Pyrus serotina Rehd." Mol Gen Genet. 241. 17-25 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sassa, H.: "Identification of self-incompatibility-related glycoproteins in styles of apple (Malus x domestica)." Theor Appl Genet. 89. 201-205 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sassa, H., H.Hirano and H.Ikehashi (1993): "Identification and characterization of stylar glycoproteins associtated with self-incompatibility genes of Japanese pear, Pyrus serotina Rehd." Mol Gen Genet. 241. 17-25

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sassa, H., H.Mase, H.Hirano and H.Ikehashi (1994): "Identification of self-incompatibility-related glycoproteins in styles of apple (Malus x domestica) ." Theor Appl Genet. 89. 201-205

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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