1994 Fiscal Year Final Research Report Summary

Analysis and indentification of proteins related to self-incompatibility types in tree crops

Research Project

Project/Area Number	05806001
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Breeding science
Research Institution	KYOTO UNIVERSITY
Principal Investigator	IKEHASHI Hiroshi Kyoto Univ.Fac.Agriculture, Professor, 農学部, 教授 (50193222)
Project Period (FY)	1993 – 1994
Keywords	self-imcompatibility / Japanese pear / Apple / RNase / Glyco-protein / Breeding
Research Abstract	Using several varieties of Japanese pear (Pyrus serotina Rehd.) , each of which has known genotype in regards to self-incompatibility, proteins extracted from the stylars were analyzed by two dimensional gel elecctro-phoresis. As the results, self-incompatibility-related RNases which cosegregate with S-genotypes were identified. The RNases were dificient in a self-compatible mutant of Japanese pear. N-terminal aminoacid sequences of the RNases showed clear homology with S-RNases of Petunia. Applying the above results, self-incompatibility-related glycoproteins in styles of apple (Malus x domestica) were identified. The self-incompatibility genotypes for more than ten varieties were determined. Self-incompatibility-related RNases in Pyrus communis were studied. Loss of one of S-RNases was confirmed in self-compatible cultivars.