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1994 Fiscal Year Final Research Report Summary

Immunocytochemical distribution of a glycoconjugate in the anterior pituitary of the rats

Research Project

Project/Area Number 05807002
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionJuntendo University

Principal Investigator

NISHIZUKA Masako  Juntendo University School of Medicine Assistant Professor, 医学部, 講師 (20133332)

Project Period (FY) 1993 – 1994
Keywordsanterior pituitary / gonadotropin / estrogen / エストロゲン
Research Abstract

Distribution of a glycoconjugate in the anterior pituitary of the rat was studied using with a monoclonal antibody that was raised against a fraction of phosphate-buffered saline-soluble proteoglycans extracted from the cerebral tissue of early postnatal rats. The immunoreactivity was seen in a considerable number of glandular cells of the anterior pituitary and in the nerve fibers in the posterior pituitary of adult rats, whereas it wasn't in the intermediate lobe. The anterior pituitary of fetal rats was immunonegative to the antibody. In neonatal male and female rats, a weak immunoreactivity was seen. A double labeling using the antibody together with an antiserum raised against beta-subunit of luteinizing hormone revealed that a sub-population of gonadotrophs in the anterior pituitary was immunoreactive in adult female rats. The immunoreactivity in these cells was localized in the secretory granules. Gonadectomy diminished the immunoreactivity in these cells, whereasan injection of estradiol-17beta restored it. Gonadotrophs in male rats lacked the immunoreactivity, but some wereimmunoreactive in estradiol-treated males. Administrations of testosterone orprogesterone did not affect the appearance of the immunoreaction in the anterior pituitary of male and female rats. These results indicate that the antigen that was recognized by the antibody in the anterior pituitary is one ofthe estrogen-induced proteins and it is involved in the reproductive function in the rat.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Nishizuka,M.: "Formation of neurons in the sexually dimorphic anteroventral periventricular nucleus of the preoptic area of the rat:Effects of prenatal treatment with testosterone propionate." Journal of Neuroendocrinology. 5(5). 569-573 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada,R.: "Persistence of reorganized synaptic connectivity in the amygdala of kindlet rats." Brain Research Bulletin. 31(6). 631-635 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumida,H.: "Sex differences in the anteroventral periventricular nucleus of the preoptic area and in the realated effects of androgen in prenatal rats." Neuroscience Letters. 151(1). 41-44 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai,Y.: "Androgen enhances neuronal degeneration in the developing preoptic area:apoptosis in the anteroventral periventricular nucleus(AVPvN-POA)." Hormones and Behavior. 28(3). 313-319 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishizuka,M.: "Chondroitin sulfate proteoglycans in the central and peripheral nervos system of the rat." Anatomical Record. Suppl1. 89- (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishizuka,M.: "Distribution of proteoglycan and glycosaminoglycans in the olfactory epithelium and nerve in chick embryos," Neuroscince Research. Suppl.18. S107- (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai,Y.: "The Development of Sex Differences and Similarities in Behavior" Haug,M.Kluwer Academic Publishers, 13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishizuka, M.: "Chondroitin sulfate proteoglycans in the central and peripheral nervous system of the rat." Anatomical Record. Suppl.1. 89 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuka, M., Ikeda, S., Arai, Y.and Maeda, N.: "Distribution of proteoglycan and alycosaminoglycans in the olfactory epithelium and nerve in chick embryos." Neuroscience Research. S18. S107 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuka, M., H.Sumida, Y.Kano, and Y.Arai: "Formation of neurons in the sexually dimorphic anteroventral periventricular nucleus of the preoptic area of the rat : Effects of prenatal treatment with testosterone propionate." J.Neuroendoeri.5(5). 569-573 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, R., M.Nishizuka, R.Iizuka, and Y.Arai: "Persistence of reorganized synaptic connectivity in the amygdala of kindled rats." Brain Res.Bull.31(6). 631-635 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sumida, H., M.Nishizuka, Y.Kano, and Y.Arai: "Sex differences in the anteroventral periventricular nucleus of the preoptic area and in the related effects of androgen in prenatal rats." Neuroscience Letters. 151(1). 41-44 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda, S., Nishizuka, M., Harigaya, T.and Arai, Y.: "Distribution of a proteoglycan and gonadotropin-releasing hormone-containing cells in the olfactory epithelium and the olfactory nerve." Proc.Jpn.Soc.Comp.Endocri.8. 12 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai Y., S.Murakami and M.Nishizuka: "Androgen enhances neuronal degeneration in the developing preoptic area : apoptosis in the anteroventral periventricular nucleus (AVPvN-POA)." Hormones and Behavior. 28(3). 313-319 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai Y., M.Nishizuka, S.Murakami, M.Miyakawa, M.Machida, H.Takeuchi and H.Sumida: "Morphological correlates of neuronal plasticity togonadal steroids : Sexual differentiation of the preoptic area." The Development of Sex Differences and Similarities in Behavior, eds., Haug, M.et al., Kluwer Academic Publishers. 311-323 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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