1995 Fiscal Year Final Research Report Summary
Analysis of requlatory mechanism for cell proliferation by modification of gangliosides
| Project/Area Number |
05807018
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Nagasaki University |
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Principal Investigator |
FURUKAWA Koichi Nagasaki University, School of Medicine, Department of Oncology, Associate Professor, 医学部, 助教授 (80211530)
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| Project Period (FY) |
1993 – 1994
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| Keywords | ganglioside / glycosyltransferase / transfection / adhesion / proliferation |
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| Research Abstract |
Using transfectant cells by GM2/GD2 synthase cDNA,we have analyzed the relationship of modified cerbohydrates with cellular proliferation, and obtained results as summarized below.
1. B16 melanoma lines newly expressing GM2 showed earlier reduction of cell growth comparing with non-expressing lines, but tumor growth in vivo did not show definite difference between GM2-expressants and non-expressants.
2. When we analyzed the cell adhesion to extracellular matrix, GM2-expressing cell lines showed high rate of adhesion to collagen type IV,fibrinectin and laminin, suggesting the promoting effects of GM2 in cell adhesion.
3. In the adhesion experiments, addition of anti-GM2 antibody decreased the adhesion of GM2-expressing cells, indicating the direct role of GM2 in cell adhesion.
4. In Northern blotting, c-myc mRNA was expressed more in the GM2-expressing cells than in non-expressants.13EA06 : Thus, newly expressed GM2 affects not only cell proliferation and adhesion, but ontracellular molecules relevant with cell proliferation.
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