1994 Fiscal Year Final Research Report Summary
T cell activation by the exotoxins having superantigenic properties and abonormal reactions observed in patients with the toxin-associated illness.
| Project/Area Number |
05807023
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
IMANISHI Ken'ichi School of Medicin, Tokyo Women's Medical College Assistant Professor, 医学部, 講師 (20132920)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMAKI Wakae School of Medicin, Tokyo Women's Medical College Instructor, 医学部, 助手 (90256496)
KATO Hidehito School of Medicin, Tokyo Women's Medical College Instructor, 医学部, 助手 (00241084)
UCHIYAMA Takehiko School of Medicin, Tokyo Women's Medical College Professor, 医学部, 教授 (00050550)
FUJIMAKI Wakae School of Medicin, Tokyo Women's Medical College Instructor (90256496)
FUJIMAKI Wakae School of Medicin, Tokyo Women's Medical College Instructor (90256496)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Superantigen / Exotoxin / T cell activation / abnormal reaction / Toxic shock syndrome |
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| Research Abstract |
Most of bacterial superantigens (SAg), such as toxic shock syndrome toxin-1 (TSST-1), staphylococcal enterotoxins (SE), and streptococcal pyrogenic exotoxins (SPE), are implicated in pathogenesis of certain infectious diseases manifesting acute and systemic clinical symptoms. We have considered that T cell activation by bacterial SAg is primarily involved in the pathogenesis of the infectious diseases caused by these bacteria. We demonstrated that these toxins activate a large number of T cells in a T-cell receptor Vbeta selective way in the presence of accessory cells (AC) expressing MHC class II molecules. The results in this project are as follows :
1. The expression of MHC class II molecules on AC is required for SAg-induced TNF-alpha and TNF-beta production in human T cells.
2.T cells is much higher than that of CD8^+ T cells is much higher thant that of CD8^+ T cells in SAg-induced TNF-alpha and TNF-beta production.
3. IFN-g-stimulated human vascular endothelial cells function as AC for SAg-induced TNF-alpha and TNF-beta production in human T cells.
4. We purified and characterized a novel mitogenic substance (YPM) produced by Yersinia pseudotuberculosis isolated from patients manifesting acute and systemic symptoms.
5. YPM is a potent T cell activator and has superantigenic properites.
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