1994 Fiscal Year Final Research Report Summary
A novel gene therapy against human leukemias : suppression of protein-tyrosine kinase activities
| Project/Area Number |
05807092
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical School (1994)
The University of Tokyo (1993) |
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Principal Investigator |
MANO Hiroyuki Jichi Medical School, Dept.Molecular Biology, Assistant professor, 医学部, 講師 (90240704)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Hiroaki Jichi Medical School, Dept.Molecular Biology, Assistant professor, 医学部, 助手 (40245064)
SAKAI Ryuichi Jichi Medical School, Dept.Molecular Biology, Associate professor, 医学部, 助手 (40215603)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Protein-tyrosine kinase / Anti-sense |
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| Research Abstract |
Protein-tyrosine kinase(PTK)is known to be directly involved in the mechanisms of cell growth or differentiation. As in the case of the Bcr-Abl kinase in chronic myeloid leukemia(CML), activated PTKs have been identified in some human malignancies and shown to participate in the process of oncogenesis. In this study, we have investigated if suppression of such PTK-activities may block the deregulated growth of cancer cells. Tec PTK is known to transduce mitogenic stimuli of cytokines. Addition of antisense oligonucleotides against Tec to culture medium was demonstrated to suppress the cytokine-dependent growth of both leukemia cells and normal bone marrow mononuclear cells. Also, antisense oligonucleotides against Abl could block the growth of CML cell line in a sequence-specific manner.Taken together, suppression of PTK activities should be a novel approach of gene therapy against a broad range of malignancies.
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