1994 Fiscal Year Final Research Report Summary
Significance and functional analysis of protein-tyrosine phosphatases expressed in hematopoietic disorders.
| Project/Area Number |
05807096
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Sapporo Medical University School of Midicine |
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Principal Investigator |
ADACHI Masaaki Sapporo Medical University, School of Medicine 1st Deparment of Internal Medicine Instructor, 医学部, 助手 (70240926)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Kohzoh Sapporo Medical University, School of Medicine 1st Deparment of Internal Medicin, 医学部, 教授 (60117603)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Protein-tyrosine phosphatase / LC-PTP / SHPTP2 / IL-2 / Chromosome assignment / genome DNA / tyrosine kinase |
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| Research Abstract |
Protein-tyrosine phosphatases (PTPs) are known to play a role in removal of phosphate groups from phosphotyrosines, which accumulate after activation of tyrosine kinases. We have already isolated cDNAs encoding PTPs expressed in hematopoietic cells. Here, we investigate the abnormalities of their genomic structures and expressions to see the correlation of PTPs with oncogenesis. We obtained the results as described below.
We isolated LC-PTP genomic clones and demonstrated the genomic organization. LC-PTP gene consists of 11 exons and its organizations are similar to CD45 gene (transmembrane PTP). From this study, we can analyze the somatic mutations in LC-PTP gene by PCR method.
We found LC-PTP gene expression is induced by IL-2 stimulation, and demonstrated that LC-PTP gene is an early response gene. This data suggests that LC-PTP can function in T-cell proliferation.
We have mapped SH-PTP gene. SH-PTP gene is localized at chromosome 12q24.1 This locus is knowm to be associated with chromosomal abnormalities in chronic lymphocytic leukemia (CLL), germ cell tumors, and leiomyoma. It is of interest that functional analyzes of SH-PTP2 in the disorders.
We generated polyclonal antibody to SH-PTP2, and see localization of SHPTP2 expressions. We found SHPTP2 is highly expressed in vascular smooth muscle cells ubiquitously. We are investigating SHPTP2 functions in cardiovascular system.
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