1994 Fiscal Year Final Research Report Summary
A study of liver cryopreservation
| Project/Area Number |
05807168
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児外科
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| Research Institution | Nagoya City University |
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Principal Investigator |
MASHITA Keiji Nagoya City University Medical School, assistant, 医学部, 助手 (10181637)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAI Tomoyuki Nagoya City University Medical School, professor, 医学部, 教授 (60080066)
HASHIMOTO Takashi Nagoya City University Medical School, assistant professor, 医学部, 助教授 (10094393)
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| Project Period (FY) |
1993 – 1994
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| Keywords | liver preservation / bile production / liver perfusion / cryopreservation |
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| Research Abstract |
An ex vivo isolated perfused rat liver model was tested to assess viability of preserved liver.
Rats were pretreated with or without papaverine hydrochloride. Livers were taken out and perfused with TOM-2H organ preservation solution (made by Dr.IWANAKA,Tokyo University) and stored 4ﾟC before reperfusion with Krebs Ringel Bicarbonate Solution at 37ﾟC in a noncirculating perfusion system for l hr. From 30 mins after reperfusion, Taurocholate added into perfusion solution and examined bile flow, bile salt output, lactate dehydrogenase level every 5 mins during perfusion. Papaverine hydrochloride-pretreated livers produced significantly more bile flow and bile salt output than control liver s and also released significantly less lactate dehydrogenase level into the perfusate after reperfusion. Pathological finding shows destroyed ZONE 3 (pericentral area) in control livers, but it was not seen in papaverine hydrochloride-pretreated livers.
We supposed papaverine hydrochloride prevent vasoconstriction during preservation.
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