1994 Fiscal Year Final Research Report Summary
Regulation of catecholamine neurotransmitterlevels in the extracellular fluid by cyclic AMP
| Project/Area Number |
05807173
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
NAKANISHI Nobuo Meikai Univ., Biochem., Assistant Prof., 歯学部, 講師 (20118574)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Cyclic AMP / Protein phosphorylation / Catecholamine uptake / Vesicular amine transport / Neurotransmitter / Extracellular amine / Pheochromocytoma PC12 / Dopaminergic neuron |
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| Research Abstract |
We had found that cyclic AMP (cAMP) inhibited catecholamine transport and increased the extracellular dopamine level in pheochromocytoma PC12 cell culture, a model for monoaminergic neurons. An aim of the present study is to clarify whether cAMP regulates the monoamine transport and extracellular monoamine neurotransmitter in the CNS and PNS.With using a brain microdialysis technique, we examined cAMP effect on extracellular dopamine level in striatum regions of freely moving rat. Mechanisms of this cAMP action on the monoamine transport was also examined with PC12 cells in culture.
Dialytic infusion of dibutyryl cAMP (dBcAMP) into rat striatum resulted in an increase in extracellular dopamine level and this dBcAMP effect was detectable under the low Ca^<2+> concentration in the extracellular fluid where the dopamine increase was not observed by the infusion of striatum with high K^+ (50mM). Comparison of the dBcAMP effect on extracellular dopamine with monoamine transport inhibiors, nomifensine and reserpine, also supported the idea that cAMP increases extracellular dopamine by inhibiting the amine transport in dopaminergic neurons in the striatum. Effect of dBcAMP on a level of dihydroxyphenylacetic acid (DOPAC), a dopamine-derived metabolite, was also examined and was compared with those of the monoamine transport inhibitors. dBcAMP showed an effect similar to that of reserpine, suggesting the inhibition of monoamine transport through secretory vesicular membrane by dBcAMP.With PC12 cells, we confirmed that cAMP inhibited catecholamine reuptake and increased extracellular dopamine by down-regulating the vesicular monoamine transport.
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Research Products
(21 results)
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[Publications] NAKANISHI,NObuo: "Long-term treatment of PC12 pheochromocytoma with dibutyryl cyclic AMP increases biopterin content in the cells but decreases that in the medium(In:Chemistry and Biology of Pteridines and Folates,pp.231-234)" Plenum Press,New York(Ayling,J.E.,Nair,M.G.,&Baugh,C.M.,eds.), 4 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] NAKANISHI,N., MATSUMOTO,R., OHSHIRO,I., ISONO,A., HARA,M., ITAGAKI,K., TAKAESU,M.and MINAMI,N.: "Effect of dibutyryl cyclic AMP and protein synthesis inhibition on intra-and extracellular biopterin in pheochromocytoma PC12 cells in culture" J.Meikai Univ.Sch.Dent.24 (in press). (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] NAKANISHI,N., MATSUMOTO,R., OHSHIRO,I., ISONO,A., HARA,M., ITAGAKI,K., TAKAESU,M.and MINAMI,N.: "Effect of nomifensine on dopamine and biopterin contents in PC12 pheochromocytoma culture" J.Meikai Univ.Sch.Dent.24 (in press). (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] ITAGAKI,K., TAKAESU,M., OSHIRO,I., OKAMOTO,A., OKAZAKI,T., ITO,M., MATSUMOTO,R., NAKANISHI,N.and MINAMI,N.: "Regulation of dopa and dopamine production of PC12 cells in vivo : Effect of aromatic amino acids and catechols" Meikai Univ.Sch.Dent.24 (in press). (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] NAKANISHI,N., ONOZAWA,S., ISONO,A., HARA,M., HASEGAWA,H., and YAMADA,S.: "Long-term treatment of PC12 pheochromocytoma with dibutyryl cyclic AMP increases biopterin content in the cells but decreases that in the medium" Chemistry and Biology of Pteridines and Folates (Ayling, J.E., et al.eds.) pp. 231-234, Plenum Press, New York. (1993)
Description
「研究成果報告書概要(欧文)」より
