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1996 Fiscal Year Final Research Report Summary

Visualized measurement of cell by laser and fluorescence dye, -Its application to immunology and cancer treatment-

Research Project

Project/Area Number 06044027
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionIbaraki University

Principal Investigator

FUJII Kanichi  Ibaraki University, Professor, 工学部, 教授 (00054354)

Co-Investigator(Kenkyū-buntansha) WHINNERY R.J  University of California at Burkely, Professor, 工学部, 教授
GUSTAFSON T.K  University of California at Burkely, Professor, 工学部, 教授
AMANO M  Stanford University, Research associate, 医学部, 助手
TOWNS C.H  University of California at Burkely, Professor, 理学部, 教授
MIROV S  University of Alabama at Birmingham, Associate Professor, 理学部, 助教授
SCHAWLOW A.L  Stanford University, Professor, 理学部, 教授
MC Cutcheon  アラバマ大学, 工学部, 教授
MCGHEE J.R  University of Alabama at Birmingham, Professor, 免疫ワクチンセンター, 所長教授
ISHIKAWA Satoru  Hitachi Hospital, Chief doctoer, 日立総合病院, 泌尿器科主任医長
OKA Yu-ji  Hitachi Hospital, Vice-president, 日立総合病院, 副院長 (20160658)
MEGA Junichi  Nihon University, Assistant Professor, 松戸歯学部, 講師 (40190946)
KAMEMARU Shunichi  Kitami Institute of Technology, Professor, 工学部, 教授 (60175289)
KIYONO Hiroshi  Osaka University, Professor, 微生物病研究所, 教授 (10271032)
KUBOTA Toshio  Ibaraki University, Assistant Professor, 工学部, 講師 (40143143)
TUJI Ryusuke  Ibaraki University, Associate Professor, 工学部, 助教授 (40188537)
SHIRAISHI Masatake  Ibaraki University, Professor, 工学部, 教授 (10091860)
MCCUTCHEON M  University of Alabama at Birmingham, Professor
Project Period (FY) 1994 – 1996
KeywordsILISPOT / immunoassay / cytokine / IDIP / automation / pattern recognition / laser / Fluorescent dye
Research Abstract

This project among Ibaraki University, University of Alabama at Birmingham, and Stanford University was started at 1994. The purpose of this project is thedevelopment of new automatic immunoassay system which removed human errors. Although the purpose was not accomplished perfectly, the semi-automatic system was performed.
The concrete results were summarized as follows :
1.The automation of hard-ware of ILISPOT-IDIP system by computer controll
The automatic adressing of the stage of the microscope using the combination of stepping motor and computer was accomplished. Although the image processing system is semi-sutomatic at this time, the automatic recognition between real spots and psuede spots was performed.
Concequently, it is thought that the performance of the ILISPOT-IDIP system attains relatively high level as the automatic immunoassy system.
2.The application of color center laser as the light source of ILISPOT system was thought effective for the enhancement of the ditecting limitaion of the spots. Hpwever, the instrument of color center laser is so huge. The investigation of the down-sizing of the instrument now in progress.
3.Three kinds of new fluoorine-containing fluorescent dyes (modified BODIPY) was prepared. Although the dyes have lipophilicity, solubility in water and high intensity of emmition, the study of the combination of the dyes with proteins or DNA is now in progress.

  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] 藤井 寛一: "Mode Control of Semiconductor Laser with Diffraction and Dispersion Feedback" Proceeding of 12th International Conference on Laser Interaction and Related Plasma Phenomena. part2. 849-853 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤井 寛一: "Pulse Broadning and of Copper Vapor in Copper-Halide Lasing System" Proceeding of 12th International Conference on Laser Interaction and Related Plasma Phenomena. part2. 872-877 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤井 寛一: "Enhancement of Population Inversion by Effective Use of Potential Energy in self-Termination Laser" Proceeding of 12th International Conference on Laser Interaction and Related Plasma Phenomena. part2. 878-882 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤井 寛一: "Comparison of A.C.Driven Barrier Discharge with D.C.Driven Malter Effect Corona Discharges for Plasma Reactor" Proceeding of 11th International Conference on Gas Dis charges and Their Applications. 1. 164-167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤井 寛一: "A Proposal of Modifying the Quittner Method for Gamma-Ray Peak Area Determination" Radio Isotopes. 45. 92-94 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,I.,Nakagawa,I.,Kiyono,H.,McGhee,J.R.,Clements,J.D.,and Hamada,S.: "Mucosal T cells induce systemic anergy for oral tolerance" Biochem.Biophys.Res.Commun.206. 414-420 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Beagley,K.W.,Yamamoto,M.,McGhee,J.R.and Kiyono,H.: "Differences in intraepithelial lymphocyte T cell subsets isolated from murine samll versus large intestine" J.Immunol.154. 5611-5619 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroi,T.,Fujihashi,K.,McGhee,J.R.and Kiyono,H.: "Polarized Th2 cytokine expression by both mucosal γδ and αβ T cells." Eur.J.Immunol.25. 2743-2751 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bukawa,H.,Sekigawa,K-I.,Hamajima,K.,Fukuoka,J.,Yamada,Y.,Kiyono,H.and Okuda,K.: "Neutralization of HIV-1 secretory IgA induced by oral immunization with a new macromolecular multicomponent peptide vaccine candidate." Nature Medicine. 1. 681-685 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Marinaro,M.,Staats,H.F.,Hiroi T.,Kiyono,H.,Jackson,R.J.,Coste,M.and McGhee,J.R.: "The Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4 which supports IgE responses" J.Immunol.155 : 4621-4629. 155. 4621-4629 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,I.,Kiyono,H.,Marinaro,M.,Jackson,R.J.,Nakagawa,I.,Fujihashi,K.,Hamada,S.,Clements,Bost,K.L.,and McGhee,J.R.: "Mechanisms for mucosal immunogenicity and adjuvancy of Escherichia coli labile enterotoxin" J.Infect.Dis.173. 627-635 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujihashi,K.,Yamamoto,M.,Hiroi,T.,McGhee,J.R.,and Kiyono,H.: "Selects Th1 and Th2 cyotkine mRNA expression by CD4^+ T cells isolated from inflamed human gingival tissues" Clin.Exp.Immunol.103. 422-428 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi.I.,Kiyono,H.,Jackson,R.J.,Fujihashi,K.,Staats,H.F.,Hamada,S.,Clements,J.D.,Bost,K.L.,and McGhee,J.R.: "Epitope maps of Escherichia coli labile toxin B subunit for development of a synthetic oral vaccine." Infect.Immune.64. 1290-1298 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] VanCott.,J.L.,Kobayashi,T.,Pillai,S.,McGhee,J.R.,and Kiyono,H.: "Induction of polysaccharide specific mucosal immune responses by oral immunization" Vaccine. 14. 392-398 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,M.,Kawabata.K.,Fujihashi,K.,McGhee,J.R.,Van Dyke,T.E.,Bamberg,T.V.and Kiyono,H.: "Lack of IL-4 contributes to persistence of IL-4 receptor positive macrophages in inflamed gingival tissues." Am.J.Pathol.148. 331-339 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Vancott,J.L.,Staats,H.F.,Roberts,M.,Chatifield,S.N.,Hiroi,T.,Yamamoto.,M.,Carter,P.B.,Kiyono,H.and McGhee,J.R.: "Regulation of mucosal and systemic antibody responses by T helper subsets,macrophages and derived cytokines following oral immunization with live recombinant Salmonella" J.Immunol.156. 1504-1514 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okahashi,N.,Yamamoto,M.,Vancott,J.L.,Charfield,S.N.,Roberts,M.,Bluethmann,H.,Hiroi,T.,Kiyono,H.and McGhee,J.R.: "Mucosal immunity in IL-4 knockout mice : oral administration of recombinant Salmonella or cholea toxin elicits CD4^+ Th2 cells producing IL-6 and IL-10 and IgA responses" Infect.Immun.64. 1516-1525 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jackson,R.J.,Fujihashi,K.,Kiyono,H.and McGhee,J.R.: "Luminometry : a novel bioluminescent method enhances the quatitation of mucosal and systemic antibody responses" J.Immunol.Methods.190. 189-197 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujihashi,K.,Kawabata,S.,Hiroi,T.,Yamamoto,M.,KcGhee,J.R.,Nishikawa,S.-I.and Kiyono,H.: "IL-2 and IL-7 reciprocally induce IL-7 and IL-2 receptors on γδ TCR^+ intraepithelial lymphocytes." Proc.Natl.Acad.Sci.USA.93. 3613-3618 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujihashi,K.,McGhee,J.R.,Kweon,M-N,Cooper,M.D.,Tonegawa,S.,Takahashi,I.,Hiroi,T.,Mestecky,J.and Kiyono,H.: "γδ cell deficient mice have imparied mucosal IgA responses" J.Exp.Med.183. 1929-1935 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Elson,C.O.,Beagley,K.W.,Sharmanov,A.T.,Kiyono,H.,Tennyson,G.S.,Ridwan,B.,Fujihashi,K.and McGhee,J.R.: "Hapten-induced model of murine IBD : mucosal immune responses and protection by tolerance." J.Immunol.157. 2174-2185 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakagawa,I.,Takahashi,I.,Kiyono,H.,McGhee,J.R.and Hamada,S.: "Oral Immunization with the B subnit of the heat-labile entrerotoxin of Escherichia coil induces early Th1 and Th2 cytokines expression in Peyer's patches." J.Infect.Dis.173. 1426-1428 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jackson,R.J.,Marinaro,M.,VanCott,J.L.,Yamamoto,M.,Okahashi,N.,Fujihashi,K.,Kiyono,H.,Chatifield,S.N.,and McGhee,J.R.: "Mucosal immunity : regulation by helper T cells and a novel method for datection" J.Biotech.44. 209-216 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤、亀丸: "特徴抽出に基づくマッチトフィルタリングとニューラルネットワークを用いたパターン認識" 光学. 24. 32-39 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kamemaru,K.Tanaka,M.Nakagawa: "Universal patern recognition by mathced filters synthesized by primitive patterns and by the algorithm for uniquely selecting the optimum reference patterns" Proc.of SPIE. 232-239 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kubota: "Fluorine-containing Fluorescent Dye for Immunoassay and Optical Resolving Agent from Trifuoropropene Oxide" Proc.of Pacifichem'95. 09N904 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kubota: "Separation of an Enantiomorph and its Racemate by Distillation : Strong Chiral Recognition Ability of Trifluorolactates" Chemistry Letters. 115-116 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyono,H: "Mucosal vaccine : New Trends in Immunization." Academic Press,San Diego, 479 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kagnoff,M: "Essential of Mucosal Immunology" Academic Press,San Diego, 597 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyono,H: "Igaku-no-Ayumi,Current view of host protection-mucosal immune system,." Igakushoin Pub.Co.Ltd.,Tokyo, 206 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kan-ichi Fujii: "Mode Control of Semiconductor Laser with Diffraction and Dispersion Feedback" Procceeding of 12th International Conference on Laser Interaction and Related Plasma Phenomena. part 2. 849-853 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kan-ichi Fujii: "Pulse Broadning and of Copper Vapor in Copper-Halide Lasing System" Procceeding of 12th International Conference on Laser Interaction and Related Plasma Phenomena. part 2. 872-877 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kan-ichi Fujii: "Enhancement of Population Inversion by Effective Use of Potential Energy in self-Termination Laser" Procceeding of 12th International Conference on Laser Interaction and Related Plasma Phenomena. part 2. 878-882 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kan-ichi Fujii: "Comparison of A.C.Driven Barrier Discharge with D.C.Driven Malter Effect Corona Discharges for Plasma Reactor" Procceeding of 11th International Conference on Gas Discharges and Their Applications. 1. 164-167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kan-ichi Fujii: "A Proposal of Modifying the Quittner Method for Gamma-Ray Peak Area Determination" Radio Isotopes. 45. 92-94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, I.: "Mucosal T cells induce systemic anergy for oral tolerance" Biochem.Biophys.Res.Commun.206. 414-420 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Beagley, K.W.: "Differences in intraepithelial lymphocyte T cell subsets isolated from murine samll versus large intestine" J.Immunol.154. 5611-5619 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroi, T.: "Polarized Th2 cytokine expression by both mucosal gammadelta and alphabeta T cells." Eur.J.Immunol.25. 2743-2751 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bukawa, H.: "Neutralization of HIV-1 secretory IgA induced by oral immunization with a new macromolecular multicomponent peptide vaccine candidate." Nature Medicine. 1. 681-685 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Marinaro, M.: "The Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4 which supports IgE responses" J.Immunol.155. 4621-4629 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, I.: "Mechanisms for mucosal immunogenicity and adjuvancy of Escherichia coli labile enterotoxin" J.Infect.Dis.173. 627-635 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujihashi, K.: "Selected Th1 and Th2 cyotkine mRNA expression by CD4^+ T cells isolated from inflamed human gingival tissues" Clin.Exp.Immunol.103. 422-428 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, I.: "Epitope maps of Escherichia coli labile toxin B subunit for development of a synthetic oral vaccine." Infect.Immune.64. 1290-1298 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] VanCott, J.L.: "Induction of polysaccharide specific mucosal immune responses by oral immunization" Vaccine. 14. 392-398 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, M.: "Lack of IL-4 contributes to persistence of IL-4 receptor positive macrophages in inflamed gingival tissues." Am.J.Pathol.148. 331-339 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Vancott, J.L.: "Regulation of mucosal and systemic antibody responses by T helper subsets, macrophages and derived cytokines following oral immunization with live recombinant Salmonella" J.Immunol.156. 1504-1514 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okahashi, N.: "Mucosal immunity in IL-4 knockout mice : oral administration of recombinant Salmonella or cholea toxin elicits CD4^+ Th2 cells producing IL-6 and IL-10 and IgA responses" Infect.Immun.64. 1516-1525 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jackson, R.J.: "Luminometry : a novel bioluminescent method enhances the quantitation of mucosal and systemic antibody responses" J.Immunol.Methods. 190. 189-197 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujihashi, K.: "IL-2 and IL-7 reciprocally induce IL-7 and IL-2 receptors on gammadelta TCR^+ intraepithelial lymphocytes." Proc.Natl.Acad.Sci.USA.93. 3613-3618 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujihashi, K.: "gammadelta T cell deficient mice have imparied mucosal IgA responses" J.Exp.Med.183. 1929-1935 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Elson, C.O.: "Hapten-induced model of murine IBD : mucosal immune responses and protection by tolerance." J.Immunol.157. 2174-2185 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakagawa, I.: "Oral Immunization with the B subnit of the heat-labile entrerotoxin of Escherichia coil induces early Th1 and Th2 cytokines expression in Peyer's patches." J.Infect.Dis.173. 1426-1428 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jackson, R.J.: "Mucosal immunity : regulation by helper T cells and a novel method for datection" J.Biotech.44. 209-216 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kamemaru: "Universal patern recognition by matched filters synthesized by primitive patterns and by the algorithm for uniquely selecting the optimum reference patterns" Proc.of SPIE. 2565. 232-239 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kubota: "Fluorine-containing Fluorescent Dye for Immunoassay and Optical Resolving Agent from Trifluoropropene Oxide" Proc.of Pacifichem '95. 09N904 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kubota: "Separation of an Enentiomorph and its Racemate by Distilllation : Strong Chiral Recognition Ability of Trifluorolactates" Chemistry Letters. 115-116 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyono, H: Academic Press, San Diego. Mucosal vaccine : New Trends in Immunization., 1-479 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kagnoff, M: Academic Press, San Diego. Essential of Mucosal Immunology, 1-597 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyono, H: Igakushoin Pub.Co.Ltd., Tokyo. Igaku-no-Ayumi, Current view of host protection-mucosal immune system, 1-206 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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