1995 Fiscal Year Final Research Report Summary
Transgenic analysis of the expression of OPN gene-encoding a molecule responsible for cancer cell attachment.
| Project/Area Number |
06044073
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NODA Masaki Medical Research Institule, Tokyo Medical and Dental University, Professor, 難治疾患研究所, 教授 (50231725)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAGUTI Nanako Tokyo Medical and Dental University, 難治疾患研究所, 助手 (10200700)
DENHARDT David Rutgers University, ネルソン生物学研究所, 所長
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| Project Period (FY) |
1994 – 1995
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| Keywords | Osteopontin / Osteoblat / Bone Morphogenetic Protein / Bone Formation / Bone Protein / Osteoclast / gene expression / Differentiation |
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| Research Abstract |
To eluidate the role of osteopontinm, we prepared to examine the effect of osteopontin overexpression as well as inactivation of osteopontin gene in mice system. We first cloned murine osteopontin gene and constructed vectors by using the upstream exons. These constructs also contained neo-gene cassett and were introduced into murine ES cells by electroporation. ES cells containing homologous recombination in the osteopontin gene were selected and they were introduced into the blastocele. Chymeric mice were identified by their coat color and were mated to prodece mice which lack osteopontin gene through homolo gous recombination. With regard to over expression experiments, osteopontin gene was cloned down stream of a strong promoter to yield inducible expression vectors and this vector was introduced via microinjection into fertilized eggs which were subsequently implanted into the uterus of pseudo-pregnant mice.
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