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1995 Fiscal Year Final Research Report Summary

Functional Analysis of Recombination Proteins in Eukaryotes

Research Project

Project/Area Number 06044142
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNATIONAL INSTITUTE OF GENETICS (1995)
Osaka University (1994)

Principal Investigator

OGAWA Tomoko  Department of Cell Genetics, National Institute of Genetics Professor, 細胞遺伝研究系, 教授 (80028208)

Co-Investigator(Kenkyū-buntansha) エーゲルマン エドワード  ミネソタ大学, 細胞生物学部門, 教授
ビショップ ダグラス  シカゴ大学, 細胞照射線腫瘍部門, 講師
SHINOHARA Akira  Faculty of Science, Osaka University, 理学部, 助手 (00252578)
OGAWA Hideyuki  Faculty of Science, Osaka University, 理学部, 教授 (70028207)
BISHOP Douglas K.  Department of Radiation and Cellular Oncology, Medical School, University of Chi
EGELMAN Edward H.  Department of Cell Biology, Medical School, Minnesota University
Project Period (FY) 1994 – 1995
KeywordsMeiotic Recombination / Rad52 Protein / Lim15 (Dmc1) Protein / Chromosome Structure / Double Strand Breaks / Initiation of Recombination / ssDNA Binding Protein (RPA) / Interaction of Recombination Protein
Research Abstract

In meiosis, haploid gametes are produced from diploid parental cells.In most organisms, this reductional segregation of chromosomes accompanies with genetic recombination between homologous chromosomes.The recombination that occurs in meiotic prophase 1 provides for proper distribution of chromosomes and for generation of genomic diversity.
During meiosis, chromosomes synapse and recombine using various complexes formed by recombination proteins and show a series of morphological changes.To understand the mechanisms of the recombination, we are studying the mechanisms of action of each protein that constitutes in the complexes involved in recombination and repair of damaged DNA in yeast.
By using the mouse and lily chromosomes in meiosis, we studied the relationships between morphological changes of chromosomes and the localization of the recombination proteins.We found that the Rad51 and Lim 15 proteins, that have general properties of searching and pairing of homologous DNA sequences, localize at specific positions at specific time.Both proteins on meiotic chromosomes of lily showed that these proteins participate in searching for homologous chromosomes on the leptotene stage and lead to chromosome pairing in zygotene stage.In mouse meiotic chromosomes, the Rad 51 protein becomes a component of the synaptonemal complex core in pachytene chromosomes and is involved in chiasma formation in the diplotene.On the other hand, the Lim15 protein is present only at both ends of cores in the pachytene through diplotene chromosomes even in diakinesis.The Lim15 protein is not involved in chiasma formation and instead, is involved in recombination in the telomeric region and in segregation of paired chromosomes in prophase 1.thus, it is evident that Rad51 and Lim15 play different roles in the late stages of meiotic recombination.

  • Research Products

    (67 results)

All Other

All Publications (67 results)

  • [Publications] Hitoshi Kurumizawa, Shukuko Ikawa, Tomoatsu Ikeya, Tomoko Ogawa and Takehiko Shibata: "A chimera RecA protein exhibits altered double-stranded DNA-binding." J.Biol.Chem.269. 3068-3075 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomoko Ogawa, Akira Shinohara, Akira Nabetani, Tomoatsu Ikeya, Xiong Yu, Edward H.Egelman and Hideyuki Ogawa: "RecA-like Recombination Proteins in Eukaryotes : Functions and Structures of RAD51 Genes." Cold Spring Harbor Symp.Quant.Biol.58. 567-576 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hitoshi Kurumizawa, B.J.Rao, Tomoko Ogawa, Charles M.Radding and Takehiko Shibata,: "A chimeric RecA protein that implicates non-Watson-Crick interaction in homologous pairing" Nucleic Acid Res.22. 3387-3391 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomoko Ogawa Akira Shinohara and Tomoatsu Ikeya: "A species-specific interaction of Rad51 and Rad52 proteins" Adv.Biophysics,. 31. 93-100 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiro Terasawa, Akira Shinohara, Yasuo Hotta, Hideyuki Ogawa, and Tomoko Ogawa: "Localization of RecA-like Recombination Proteins on Chromosomes of Lily at Various Meiotic Stages." Genes & Development. 9. 925-934 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Mikawa, R.Masui, T.Ogawa, H.Ogawa and S.Kuramitsu: "N-torminal 33 amino acid residues of Escherichia coli RecA protein contribute to its self-assembly." J.Mol.Biol.250. 471-483 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Shinohara and T.Ogawa: "Homologous recombination : Role of doubl-strand breaks" TiBS, “DNA Repair"A secial issue 22GD07:20. 387-391 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ikeya, A.Shinohara, S.Sato, S.Tabata and T.Ogawa: "Localization of Mouse Rad51 and Lim 15 on Meiotic Chromosomes at Late Stages of Prophase 1" Genes to Cells. 1(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Sinohara, M.Shinohara and T.Ogawa: "Roles of Rad52 Protein of Saccharomyces cerevisiae in Homologous Recombination" Cell. Submitted. (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ryuichi Kato and Hideyuki Ogawa: "An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae" Nucleic Acids Research. 22. 3104-3112 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsyki Johzuka Hideyuki Ogawa: "Interaction of Mre11 and Rad5- : Two protein required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae" Genetics. 139(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideyuki Ogawa, Katsuki Johzaka, Takuro Nakagawa, Sunhee, Leem and A.H.Hagiwara: "Functions of the yeast meiotic recombination genes, MRE11 and MRE2" Adv.Biophysics. 31. 67-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhiro Maeshima, Katsumi Morimatsu, Akira Shinohara and Toshihiro Horii20GB13:Rad51 homologues in Xenopus leavis : two distinct genes and highly expressed in ovary and testis.: Gene. in press. 67-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dougras Bishop: "Rec51 homologs Dmc1 and Rad51 interact to form multiple nuclear complexes before meiotic chromosome synapsis." Cell. 79. 1081-1092 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Stasiak, A.and Egelman, E.H.: "Structure and Function of RecA-DNA Complexes" Experientia. 50. 192-203 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bednar, J., Furrer, P., Stasiak, A., Dubochet, J., Egelman, E.H.and Bates, A.D.: "The twist, writhe and overall shape of supercoiled DNA changeduring counterion induced transition from a loosely to a tightly interwoundsuperhelix.Possible implications for DNA structure in vivo." J.Mol.Biol.235. 825-847 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Orlova, A., Yu, X.and Egelman, E.H.: "Three-dimensionalReconstruction of a Co-complex of F-Actin with Antibody Fab Fragments toActin's Amino Terminus." Biophysical Journal. 66. 276-285 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Egelman, E.H.: "The Ghost of Ribbons Past." Current Biology. 4. 79-81 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Stasiak, A., Tsaneva, I., West, S.C., Benson, K., Yu, X.and Egelman, E.H.20GB19:The E.coli RuvB Branch Migration Protein Forms Double Hexameric Rings Around DNA: Proc.Natl.Acad.Sci.U.S.A. 91. 7618-7622 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuprun, V., Anderson, D.and Egelman, E.H: "The Bacteriophage 129 Head-Tail Connector Shows 13-Fold Symmetry in Both Hexagonally-Packed Arrays and as Single Particles" Biophysical Journal. 66. 2139-2150 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Egelman, E.H., Yu, X., Wild, R., Hingorani, M.M.and Patel, S.S: "T7 Helicase/Primase Proteins Form Rings Around Single-Stranded DNA that Suggest a General Structure for Hexameric Helicases" Proc.Natl.Acad.Sci.U.S.A. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Egelman, E.H..: "New Insights On F-actin Dynamics.21GC22:Current Opinion in Structural Biology(invited review)" (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Orlova, A.and Egelman, E.H: "Structural Dynamics of F-actin.I.Changes in the C-terminus" J.Mol.Biol.245. 582-597 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Orlova, A., Prochniewicz, E. and Egelman, E.H: "“Structural Dynamics of F-actin.II.Cooperativity in Structural Transitions"." J.Mol.Biol.245. 598-607 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nojima, D., Linck, R.and Egelman, E.H.: "At least One of the Protofilaments of the Axonemal Doublet Microtubule is Not Made From Tubulin." Current Biology. 5. 158-167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Egelman, E.H.and Orlova, A.: "New Insights Into Actin Filament Dynamics" Current Opinion in Structural Biology. 5. 172-180 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Egelman, E.H.: "Structural Studies of Tubulin-Based Motility Race Ahead" Current Biology. 5. 1354-1356 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu, X., Angov, E., Camerini-Otero, R.D.and Egelman, E.H.: "Structural Polymorphism of the RecA Protein from the Thermophilic Bacterium T.Aquaticus" Biophysical J.69. 2728-2738 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Egelman, E.H.and Orlova, A.: "Allostery, Cooperativity and Different Structural States in F-Actin" Journal of Structural Biology. 115. 159-162 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu, X., Jezewska, M.J., Bujalowski, W.and Egelman, E.H.: "The E.coli“DnaB Helicase Exists in Several Different Hexameric States" J.Mol.Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu, X., Hingorani, M., Patel, S.S. and Egelman, E.H.: "DNA Binds Asymmetrically to One or Two Subunits of Bacteriophage T7 gp4b Hexameric-Helicase" J.Mol.Biol.submitted. (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小川智子 篠原 彰 小川 英行: "真核生物の組換え蛋白質Rad51とRad52の機能と構造" 実験医学(羊土社)「トピックス」. 12. 524-547 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小川 英行 小川 智子: "酵母に観る最新の真核性物象 VI.組換え機構 減数分裂期組換え-関与する遺伝子とその普遍性-" 蛋白質 核酸 酵素(共立出版). 39臨時増刊号. 568-578 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 篠原 彰 小川 英行 小川智子: "真核生物の組換え(修復)に働く遺伝子" 細胞工学(秀潤社). 13. 710-719 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小川智子: "真核生物の組換え蛋白質の機能と構造" 第25回 放射性総合医学研究所シンポジウム. 25. 25-37 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 篠原 彰 小川 英行 小川智子: "真核生物RAD51遺伝子の構造と機能" 日本臨床(日本臨床社). 53. 239-249 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 荻原(林)亜紀、小川 英行: "相同性組換えの機構" 実験医学. 13(増刊). 1243-1251 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小川 英行編著: "Japan Scientific Societies and Elsevier." Advances in Biophysics : Molecular Mechanisms of Genetic Recombination. 1-238 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hitoshi Kurumizaka, Shukuko Ikawa, Tomoatsu Ikeya, Tomoko Ogawa and Takehiko Shibata: "A chimera RecA protein exhinits altered double-standed DNA-binding" J.Biol.Chem. 269. 3068-3079 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomoko Ogawa, Akira Shinohara, Akira Nabetani, Tomoatsu Ikeya, Xiong Yu, Edward H.Egelman and Hideyuki Ogawa: "RecA-like Recombination Proteins in Eukaryotes : Functions and Structures of RAD51 Genes" Cold Spring Harbor Symp.Quant.Biol. 58. 567-576 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hitoshi Kurumizaka, B.J.Rao, Tomoko Ogawa, Charles M.Radding and Takehiko Shibata: "A chimeric RecA protein that implicates non-Watson-Crick interaction in homologous pairing" Nucleic Acid Res.22. 3387-3391 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TOMOKO Ogawa Akira Shinohara and Tomoatsu Ikeya: "A species-specific interaction of Rad51 and Rad52 proteins" Adv.Biophysics. 31. 93-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiro Terasawa, Akira Shinohara, Yasuo Hotta, Hideyuki Ogawa and Tomoko Ogawa.: "Localization of RecA-like Recombination proteins on Chromosomes of Lily at Various Meiotic Stages" Genes & Development. 9. 925-934 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Mikawa, R.Masui, T.Ogawa, H.Ogawa and S.Kuramitsu: "N-terminal 33 amino acid residues of Escherichia coli RecA protein contribute to its self-assembly" J.Mol.Biol.250. 471-483 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Shinohara and T.Ogawa: "Homologous recombination : Role of double-strand breaks" TiBS "DNA Repair" A secial issue. 20. 387-391 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ikeya, A.Shinohara, S.Sato, S.Tabata and T.Ogawa: "Localization of Mouse Rad51 and Lim15 on Meiotic Chromosomes at Late Stages of Prophase 1." Genes to Cells. 1 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Shinohara, M.Shinohara and T.Ogawa: "Roles of Rad52 Protein of Saccharomyces cerevisiae in Homologous Recombination." Cell. (Submitted). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryuichi Kato and Hideyuki Ogawa: "Nucleic Acid Res." An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae. 22. 3104-3112 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsyki Johzuka and Hideyuki Ogawa: "Interaction of Mre11 and Rad5- : Two protein required for DNA repair and meiosis-specific double-stand break formation in Saccharomyces cerevisiae" Genetics. 139(In press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideyuki Ogawa, Katsuki Johzuka, Takuro Nakagawa, Sun hee, Leem and A.H.Hagiwara: "Functions of the yeast meiotic recombination genes, MRE11 and MRE2" Adv.Biophysics. 31. 67-76 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Stasiak, A.and Egelman, E.H.: "Structure and Function of RecA-DAN Complexes." Experientia. 50. 192-203 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bednar, J., Furrer, P., Stasiak, A., Dubochet, J., Egelman, E.H.and Bates, A.D.: "The twist, writhe and overall shape of supercoiled DNA change during counterion induced transition from a loosely to a tightly interwound superhelix.Possible implications for DNA structure in vivo." J.Mol.Biol.235. 825-847 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Orlova, A., Yu, X.and Egelman, E.H.: "Three-dimensional Reconstruction of a Co-complex of F-Actin with Antibody Fab Fragments to Actin's Amino Terminus." Biophysical Journa. 166. 276-285 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Egelman, E.H.: "The Ghost of Ribbons Past." Current Biology. 4. 79-81 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Stasiak, A.Tsaneva, I., West, S.C., Benson, K., Yu, X.and Egelman, E.H.: "The E.coli RuvB Branch Migration Protein Forms Double Hexameric Rings Around DNA." Proc.Natl.Acad.Sci.U.S.A.91. 7618-7622 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuprun, V., Anderson, D.and Egelman, E.H.: "The Bacteriophage phi29 Head-Tail Connector Shows 13-Fold Symmetry in Both Hexagonally-Packed Arrays and as Single Particles." Biophysical Journal. 66. 2139-2150 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Egelman, E.H., Yu, X., Wild, R., Hingorani, M.M.and Patel, S.S.: "Bacteriophage T7 Helicase/Primase Proteins From Rings Around Single-Stranded DNA that Suggest a General Structure for Hexameric Helicases." Proc.Natl.Acad.Sci.U.S.A.92. 3869-3873 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Orlova, A.and Egelman, E.H.: "Structural Dynamics of F-actin.I.Changes in the C-terminus." J.Mol.Biol.245. 582-597 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Orlova, A., Prochniewicz, E.and Egelman, E.H.: "Dynamics of F-actin.II.Cooperativity in Structural Transitions." J.Mol.Biol.245. 598-597 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nojima, D., Linck, R.and Egelman, E.H.: "At Least One of the Protofilaments of the Axonemal Doublet Microtubule is Not Made From Tubulin." Current Biology. 5. 158-167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Egelman, E.H.and Orlova, A.: "New Insights Into Actin Filament Dynamics." Current Opinion in Structural Biology. 5. 172-180 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Egelman, E.H.: "Structural Studies of Tubulin-Based Motility Race Ahead" Current Biology. 5. 1354-1356 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu, X., Angov, E., Camerini-Otero, R.D.and Egelman, E.H.: "Structural Polymorphism of the RecA Protein from Thermophilic Bacterium T.Aquaticus" Biophysical J.69. 2728-2738 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Egelman, E.H.and Orlova, A.: "Allostery, Cooperativity and Different Structural States in F-Actin" ournal of Structural Biology J.115. 159-162 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu, X., Jezewska, M.J., Bujalowski, W.and Egelman, E.H.: "The E.coli "DnaB Helicase Exists in Several Different Hexameric States" J.Mol.Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu, X., Hingorani, M., Patel, S.S.and Egelman, E.H.: "DNA Binds Asymmetrically to One or Two Subunits of the Bacteriophage T7 gp4b Hexameric Hellcase" (Submitted). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.Bishop: "RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes before meiotic chromosome synapsis." Cell. 79. 1081-1092 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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