1994 Fiscal Year Annual Research Report

粘菌細胞における分化の調節機構

Research Project

Project/Area Number	06044234
Research Institution	Okazaki National Research Institutes
Principal Investigator	竹内郁夫岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)
Co-Investigator(Kenkyū-buntansha)	BONNER John プリンストン大学, 名誉教授 NANJUNDIAH V インド科学研究所, 教授 VERON Michel パスツール研究所, 教授 WILLIAMS Jef ロンドン大学, 教授田坂昌生京都大学, 理学部, 助教授 (90179680) 岡本浩二京都大学, 理学部, 教授 (10029944) 前田靖男東北大学, 理学部, 教授 (50025417)
Keywords	細胞分化 / パターン形成 / 遺伝子発現 / 細胞周期 / 細胞内情報伝達 / 細胞性粘菌
Research Abstract	1.細胞性粘菌の発生過程における細胞の分化傾向は細胞周期依存的に決定されることを明らかにし、細胞周期内の増殖/分化の分岐点(PS点)前後での遺伝子発現を調べた。その結果、PS点から分化する細胞で特異的に発現する遺伝子として3つ(Quit1,Quit2,Quit3)を見出し、Quit1はcAMPレセプター1(CAR1)を、Quit2は新規のCa^<2+>-結合性タンパク質(CAF-1)をコードしていることを示した。さらに、PS点において特異的にリン酸化レベルが変動する4種類のタンパク質(101kDa,90kDa,48kDa,32kDa phosphoproteins)を見出した。 2.発生途上にある粘菌細胞から、柄細胞への分化を誘導する物質が分泌されることを明らかにし、有機溶媒による分画ならびにGPCを用いるHPLCによって、この物質の部分精製を行った。この標品を用い、2種類の柄細胞特異遺伝子、ecmAとecmBに対するこの物質の効果を調べたところ、この物質はecmBのみに作用して発現を誘導することを明らかにした。さらに、ecmBの上流域に種々の欠損を持つ遺伝子を粘菌に導入し、これに上記の誘導物質を作用させ、この物質のecmBプロモーター上における作用領域を特定した。 3.突然変異体の解析から細胞の走化性に重要な役割を果たすERK2タンパク質の生化学的性質を調べ、これがアデニレートシクラーゼ(AC)の活性化に必須であることを明らかにした。さらに、細胞外のサイクリックAMPはそのリセプター(cAR1)によって受容され、おもにGα2サブユニットを介してERK2を一過性的に活性化することを明らかにした。活性化されたERK2はアデニルシクラーゼを活性化することによって細胞内のサイクリックAMPレベルを上昇させ、それによって活性化されるプロテインキナーゼA(PKA)がERK2を不活性化するという結果を得た。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Takuechi,I.: "Regulation of cell differentiation and pattern formation in Dictyostelium development" Int.J.Dev.Biol.38. 311-319 (1994)
[Publications] Morio,T.: "Cooperation of positively and negatively acting promoter elements determines prespore-specific transcription of Dp87 gene in Dictyostelium" Mech.Dev.45. 59-72 (1994)
[Publications] Nakao,H.: "Dictyostelium prespore-specific gene Dp87 encodes a sorus matrix protein" J.CellSci.107. 397-403 (1994)
[Publications] Saran,S.: "Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA" FEBS Lett.337. 43-47 (1994)
[Publications] Traynor,D.: "Aberrant pattern formation in myosin heavy chain mutants of Dictyostelium" Development. 120. 591-601 (1994)
[Publications] Araki,T.: "Cell-cycle-dependent sorting in the development of Dictyostelium cells" Dev.Biol.162. 221-228 (1994)
[Publications] Furukawa,T.: "K252a,a potent inhibitor of protein kinases,promotes the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.11. 69-76 (1994)
[Publications] 荒木剛: "粘菌の形態形成" 遺伝. 別冊6. 8-17 (1994)
[Publications] Abe,F.: "Precise expression of the cyclic AMP receptor 1 (CAR1) gene during transition from growth to differentiation in Dictyostelium discoideum" FEBS Lett.342. 239-241 (1994)
[Publications] Iijima,N.: "A proteinous factor mediating intercellular communication during the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.12. 61-69 (1995)
[Publications] Abe,F.: "Specific expression of a gene encoding a novel calcium-binding protein,CAF-1,during transition of Dictyostelium cells from growth to differentiation" Dev.Growth&Differ.37. in press (1995)
[Publications] Yamada,Y.: "Analysis of cellular interactions involved in differential control of prestalk genes in Dictyostelium discoideum" Dev.Biol.161. 296-301 (1994)
[Publications] Yoshida,H.: "dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum" Nuc.AcidsRes.22. 41-46 (1994)
[Publications] Kubohara,Y.: "Cytoplasmic Ca^<2+> and H^+ concentrations determine cell fate in Dictyostelium discoideum" FASEB J.8. 869-874 (1994)
[Publications] Kubohara,Y.: "Specific induction by zinc of Dictyostelium stalk cell differentiation" Exp.Cell Res.214. 367-372 (1994)
[Publications] Gaskins,C.: "Identification and functional analysis of a developmentally regulated extracellular signalregulated kinase gene in Dictyostelium discoideum" Mol.Cell.Biol.14. 6996-7012 (1994)
[Publications] Segall,J.E.: "A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium" J.Cell Biol.128. 405-413 (1995)
[Publications] Asahi,K.: "DIF-1,morphogen of Dictyostelium discoideum,induces the erythroid differentiation in maurine and human leukemia cells" Biochem.Biophys.Res.Comm.in press. (1995)

1994 Fiscal Year Annual Research Report

粘菌細胞における分化の調節機構

Principal Investigator

竹内 郁夫 岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)

Research Products

[Publications] Takuechi,I.: "Regulation of cell differentiation and pattern formation in Dictyostelium development" Int.J.Dev.Biol.38. 311-319 (1994)

[Publications] Morio,T.: "Cooperation of positively and negatively acting promoter elements determines prespore-specific transcription of Dp87 gene in Dictyostelium" Mech.Dev.45. 59-72 (1994)

[Publications] Nakao,H.: "Dictyostelium prespore-specific gene Dp87 encodes a sorus matrix protein" J.CellSci.107. 397-403 (1994)

[Publications] Saran,S.: "Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA" FEBS Lett.337. 43-47 (1994)

[Publications] Traynor,D.: "Aberrant pattern formation in myosin heavy chain mutants of Dictyostelium" Development. 120. 591-601 (1994)

[Publications] Araki,T.: "Cell-cycle-dependent sorting in the development of Dictyostelium cells" Dev.Biol.162. 221-228 (1994)

[Publications] Furukawa,T.: "K252a,a potent inhibitor of protein kinases,promotes the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.11. 69-76 (1994)

[Publications] 荒木 剛: "粘菌の形態形成" 遺伝. 別冊6. 8-17 (1994)

[Publications] Abe,F.: "Precise expression of the cyclic AMP receptor 1 (CAR1) gene during transition from growth to differentiation in Dictyostelium discoideum" FEBS Lett.342. 239-241 (1994)

[Publications] Iijima,N.: "A proteinous factor mediating intercellular communication during the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.12. 61-69 (1995)

[Publications] Abe,F.: "Specific expression of a gene encoding a novel calcium-binding protein,CAF-1,during transition of Dictyostelium cells from growth to differentiation" Dev.Growth&Differ.37. in press (1995)

[Publications] Yamada,Y.: "Analysis of cellular interactions involved in differential control of prestalk genes in Dictyostelium discoideum" Dev.Biol.161. 296-301 (1994)

[Publications] Yoshida,H.: "dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum" Nuc.AcidsRes.22. 41-46 (1994)

[Publications] Kubohara,Y.: "Cytoplasmic Ca^<2+> and H^+ concentrations determine cell fate in Dictyostelium discoideum" FASEB J.8. 869-874 (1994)

[Publications] Kubohara,Y.: "Specific induction by zinc of Dictyostelium stalk cell differentiation" Exp.Cell Res.214. 367-372 (1994)

[Publications] Gaskins,C.: "Identification and functional analysis of a developmentally regulated extracellular signalregulated kinase gene in Dictyostelium discoideum" Mol.Cell.Biol.14. 6996-7012 (1994)

[Publications] Segall,J.E.: "A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium" J.Cell Biol.128. 405-413 (1995)

[Publications] Asahi,K.: "DIF-1,morphogen of Dictyostelium discoideum,induces the erythroid differentiation in maurine and human leukemia cells" Biochem.Biophys.Res.Comm.in press. (1995)

竹内郁夫岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)

[Publications] 荒木剛: "粘菌の形態形成" 遺伝. 別冊6. 8-17 (1994)