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1996 Fiscal Year Final Research Report Summary

Molecular Epidemiological Studies on Diarrheal Viruses (Comparison between Countries)

Research Project

Project/Area Number 06044244
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionUniversity of Tokyo (1995-1996)
国立公衆衛生院 (1994)

Principal Investigator

USHIJIMA Hiroshi  Dept.of Maternal and Child Health Faculty of Medicine, Univ.of Tokyo, Professor, 医学部, 教授 (10091068)

Co-Investigator(Kenkyū-buntansha) GENTOCH J  Division of Enteric Virus, CDC,USA,Research fellow, 下痢症ウィルス, 主任研究官
IAN H Holmes  メルボルン大学, 微生物学, 教授
HAQUE H  Dept.of Microbiology National Institute, Bangladesh, Research fellow, 微生物学, 講師
SUPAWADEE J  Dept.of Microbiology, Chiang Mai University Chiang Mai. Thailand, Lecturer, 微生物学, 講師
MULLER Weg  Dept.of Biochemistry, Mainz Univ.Germany, Professor, 応用生化学, 教授
方 寅肇  国立公衆衛生院中国, 病毒研究所, 組長
BJORN GRINDE  ノルウェー公衆衛生院, ウィルス部, 室長
WEN Leying  Institute of Virology, Chinese Academy of Preventive Medicine, China, Research f, 病毒研究所, 技官
HASEGAWA Ayako  Dept.of Epidemiology, National Institute of Health Japan, Research fellow, 感染疫学部, 主任研究官 (10132896)
NISHIO Osamu  Dept.of Microbiology, Institute of Public Health, Japan, Chief, 衛生微生物学部, 室長 (40270631)
IAN H  Dept.of Microbiology, University of Melbourne, Professor
FANG Z-Y  Institute of Virology, Chinese Institute of Public Health, China, Chief
GRINDE Bjorn  Dept.of Virology, Institute of Public Health, Norway, Chief
Project Period (FY) 1994 – 1996
Keywordsdiarrheal virus / rotavirus / small round structured virus / adenovirus / astrovirus / Norwalk virus / molecular epidemiology / Asia
Research Abstract

New methods of diagnosis on diarrheal viruses and epidemiological studies on the viruses in Japan and Asia have been done. We developed latex agglutination test, magnetic beads-RT PCR and enzyme immunoassay for astroviruses in addition to rotaviruses and adenoviruses. We could have chance to use antibodies against Norwalk-like viruses. In addition, RT-PCR methods was used for astrovirus and Norwalk-like viruses from small samples. Small round structured vurises spread in outpatient clinics sporadically in winter time of 1995-1996. They were also found from Oyster. Clinically respiratory infection, convulsion, exanthema and others were recognized. Genetic analyzes of PCR products made possible to know the homologies and variations in inter- and intra-serotypes.
Intra-serotype variations of rotaviruses were examined in Asia in different years. The specific point mutations were recognized especially in intra-serotype 3. Serotype 1 is predominant in Asia recently, however serotype 2,3 and 4 … More were also found in small populations. Serotype 40 and 41 of adenoviruses were detected in about one tenth of rotaviruses. Genetic analyzes of the fiber regions changed at the specific regions in different countries.
Not only development of new methods to diagnosis of astroviruses, but also molecular epidemiological studies have been done. The capsid regions in serotype 1 to 7 were analyzed. The moleculr sizes were different in the different serotypes. N terminus was more similar in serotypes in comparison of those if C terminus. Serotypes 1,3 and 6 were close relationship, while, serotype 2,4,5 and 7 were close. Serotype 1 was dominant in Japan and Europe. Animal astroviruses were quite different from human astroviruses. Norwalk-like viruses were also found in 5-10% of diarrheal viruses. Snow mountain groups was predominant by sequencing. Norwalk group and Sapporo groups were also found. These results were same in Japan and in Asia.
We believe that the three years' studies deeply contributed to new research on diarrheal viruses. Less

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Ushijima H,Mukoyama A & Hasegawa A: "Serotyping of human rotaviruses in the Tokyo area by enzyme immunoassay with MAb and RT-PCR" J.Med.Virol. 44. 162-165 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato H.Kato N.& Ushijima H.: "Application of pulsed field gel electrophoresis typing to a nosocomical spread of Clostridium difficile in nes nates" J.Clin.Microbiol. 32. 2067-2070 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ushijma,xin ke Qin: "Detection and seqenecing of rotavirus VP7 gene foom human materials by RT-PCR" J.Clin.Microbiol. 32. 2893-2897 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konishi K,Ushijima H: "Effect of poly(I).poly(C_<12>U)(Ampligm) on enteric virus infection" Letters in Applied Microbiology. 19. 386-390 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Grinde B & Ushijima H: "Sensitive detection of group A rotariruses by immunomagaetic separation and RT-PCR" J.Virol.Method. 55. 327-338 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui H and Ushijima H: "Passive protection against rotairuo-induced dianhea of pups born to and nuised by dams fed Bifidobacterium" J.Infect.Dis. 172. 403-409 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito K & Ushijima H: "Detection of astrovirus from stoal samptes in Japan using reverse trans cription and polymerase chain reaction amplification" Micrcbiology and Immunology. 39. 825-828 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wen L.& Ushijima H: "Genetic variation in UP7 gene of human rotavirus sercrppe 2 isolated in Jopan,chima and pakistan" Microbiology and Immunology. 39. 911-915 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ushijima H & Morikawa S: "Characterization of VP4 and VP7 of a murone rotavirus (YR-1) isolated in Japan" Jpn J Med Sci Biol. 48. 237-247 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama M & Ushijima H: "Detection and sequencing of Norwalk-like viruses from stool samples in Japan using RT-PCR" Microbiology and Immunology. 40. 317-320 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishio O & Ushijima H: "Viral infection among infants with diarhea in Pakistan" J.Jpn.Assoc.International.Health. 10. 109-110 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama M & Ushijima H: "Detection and sequencing of Norwalk-like viruses from stool samples" Mucrobiology and Immunology. 40. 317-320 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yue Han-june & Ushijima H: "Detection and geuotyping of astroviruses by RT-PCR and sequencing" J.Jap.Assoc.Infect.Dis.70. 840-841 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wen L & Ushijima H: "Gencetic variations in the VP7 gene of human rotavirus serotype3 isolated in China and Japan" Arch Virol. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihara S & Ushijima H: "An Outbreak of Norwalk-like virus infection in Tokyo and Saitama in late 1995." J.Jap.Assoc.Infect Dis. 70. 840-841 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上田勇一,牛島廣治: "舞鶴地区における下痢症ウイルスの診断と臨床-特に小型球形ウイルスを中心にして-" 感染症学雑誌. 70. 1092-1097 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 牛島廣治: "ウイルス性下痢症とその関連疾患" 新興医学出版社, 158 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ushijima H,Mukoyama A & Hasegawa A: "Serotyping of human rotavirus in the Tokyo area by enzyme immunoassay with MAb and RT-PCR" J.Med.Virol. 44. 162-165 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato H,Kato N,& Ushijima H: "Application of pulsed field gel electrophoresis typing to a nosocomical spread of clostridium difficile in nesnates" J.Clin.Microbiol.32. 2067-2070 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ushijima H & Xin Ke Qin: "Detection and sequencing of rotavirus VP7 gene from human materials by RT-PCR" J.Clin.Microbiol.32. 2893-2897 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Konishi K & Ushijima H: "Effect of Poly (I), Poly (C_<12>U) (Ampligen) on enteric virus infection" Letters in Applied Microbiology. 19. 386-390 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Grinde B & Ushijima H: "Sensitive detection of group A rotaviruses by immunomagnetic separation and RT-PCR" J.Virol.Method. 55. 327-338 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui H & Ushijima H: "Passive protection against rotavirus-induced diarrhea of pups born to and nursed by dams fed Bifidobacterius" J.Infect.Dis. 172. 403-409 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito K & Ushijima H: "Ditection of astrovirus from stool samples in Japan using reverse transcription and polymerase chain reaction amplification" Microbiology and Immunology. 39. 825-828 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wen L & Ushijima H: "Genetic variation in VP7 gene of human rotavirus serotype 2 isolated in Japan, China and Pakistan." Microbiology and Immunology. 39. 911-915 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ushijima H & Morikawa S: "Characterization of VP4 and VP7 of a murine rotavirus (YR-1) isolated in Japan" Jpn.J.Med.Sci.Biol.48. 237-247 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama M & Ushijima H: "Detection and sequencing of Norwalk-like virus from stool samples in Japan using RT-PCR" Microbiology and Immunology. 40. 317-320 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishio O & Ushijima H: "Viral infection among infants with diarrea in Pakistan" J.Jpn.Assoc.International Health. 10. 109-110 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama M & Ushijima H: "Detection and sequencing of Norwalk-like viruses from stool samples" Microbiology and Immunology. 40. 317-320 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yue Han-june & Ushijima H: "Detection and genotyping of astroviruses by RT-PCR and sequencing" J.Jap.Assoc.Infect.Dis.70. 840-841 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wen L & Ushijima H: "Genetic variation in the VP7 gene of human rotavirus serotype 3 isolated in China and Japan" Arch.Virol.in press. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akihara S & Ushijima H: "An outbreak of Norwalk-like virus infection in Tokyo and Saitama in late 1995." J.Jap.Assoc.Infect.Dis.70. 840-841 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda U & Ushijima H: "Diagnosis and clinical manifestation's of diarreal virus infections in Maizuru Area from 199, to 1994-Especially focused on small round structured viruse (in Japanese)" Kansen shogakuzasshi. 70. 1092-1097 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ushijima H: Shinkou-igaku Publishing Co.Viral diarrheal diseases and their related diseases (in Japanese), 1-158 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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