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1996 Fiscal Year Final Research Report Summary

Mechanism and function of apoptosis

Research Project

Project/Area Number 06045054
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionScience University of Tokyo

Principal Investigator

TANUMA Sei-ichi  Science University of Tokyo, Professor, 理学部, 教授 (10142449)

Co-Investigator(Kenkyū-buntansha) ZANNETTA Jean Pierre  University of Louis Pasteur, Professor, 教授
Project Period (FY) 1994 – 1996
Keywordsapoptosis / DNA fragmentation / DNA endonuclease / DNase gamma / cell death
Research Abstract

Apoptosis is a mechanism for eliminating unwanted cells from the cell community of multicellular organisms. Abnormalities in the regulation of apoptosis may play a part in the aetiology of cancer, autoimmune diseases, AIDS,degenerative nerve diseases and malformation. On of the hallmarks of apoptosis is the enzymatic cleavage of genomic DNA into nucleosomal oligomers. The identification of an endonuclease responsible for apoptosis might help to explain how this cell suicide is regulated and why DNA is cleaved. Here, we found that gamma type of DNase was retained in apoptotic rat thymocyte nuclei. The mode of DNA cleavage, 3'-hydroxyl (OH) /5'-phosphoryl (P) ends, by homogeneously purified DNase gamma (Mr=33 kDa) and its Zn^<2+> sensitivity match those observed in apoptosis in thymocytes induced by irradiation or glucocorticoid treatment, indicating that this endonuclease is a central component of the thymic apoptosis machinery.

  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Sei-ichi Tanuma: "Occurence of an NAD+ glycohydrolase in bovine brain cytosol." Arch.Biochem.Biophys.308. 227-329 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Change in electrophoretic mobility of HL-60RG cells by apoptosis." Colloid and Polymer Sci.272. 487-492 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Induction of DNA Fragmentation in Human Myelogenous Leukemic Cell Lines by Sodium 5,6-Benzylidene-L-Ascorbate and its Related Compounds" Anticancer Res.14. 969-976 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Multiple forms of nuclear deoxyribonuclease in rat thymocytes." Biochem Biophys.Res.Commun.203. 3789-3797 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Sodium benzylideneascorbate induced apoptpsis in HIV-replicating U1 cells." FEBS Lett.351. 105-108 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Identification of an endonuclease responsible for apoptosis in rat thymocytes." Eur.J.Biochem.226. 23-30 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Antioxidative activity of sodium benzylidencascorbate and its adoriamycin-induced cardiac toxicity." Anticancer Res.14. 1875-1880 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "A Macrocircular ellagitannin,ocnothein B,supresses mouse mammary tumor gene expression via inhibition of poly (ADP-ribose) glycohydrolase." Biochem.Biophys.Res.Commun.210. 329-337 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Induction of autoantibodies in normal mice by injection of nucleobindin and natural occurrence of antibodies against nucleobindin in autoimmune MRL/lpr/lpr mice." Immunol.Lett.45. 35-42 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Apoptosos : a cell ellimination mechanism." Sut.Bull.12. 36-43 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Induction of apoptosis by T2 toxin and other natural toxins in HL-60 human promyelotic leukemia cells." Natural Toxins. 3. 129-137 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "DNA fragmentation in apoptosis." Tiss.Cult.Res.Commun.14. 133-140 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "An endonuclease responsible for apoptosis." Apoptosis : Prog.Mol.Subcell.Biol.Springer-Verlag. 1-12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Molecular mechanisms of apoptosis." Apoptosis in Normal Development and Cancer (ed.by Mels Sluyser),Taylor & Francis Ltd.,London UK.39-59 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Inhibitory effect of tannic acid on human immunodeficiency virus promoter activity induced by 12-0-tetradecanoylphorbol-13-acetate in Jurkat T-cells." Biochem.Biophys.Res.Commun.220. 411-417 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "DNA fragmentation during thymic apoptosis is catalyzed by DNaseγ." Apoptosis. 1. 147-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Properties of poly (ADP-ribose) glycohydrolase purified from pig testis nuclei." Arch.Biochem.Biophys.336. 139-146 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei-ichi Tanuma: "Replication factor C recognizes 5'-phosphate ends of telomeres." Biochem.Biophys.Res.Commun.229. 310-315 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田沼 靖一: "アポトーシス-細胞の生と死-" 東京大学出版会, 145 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田沼 靖一: "アポトーシス実験プロトコール" 秀潤社, 236 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田沼 靖一: "アポトーシス-機能と機構-" 日経サイエンス社, 185 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田沼 靖一: "アポトーシスの制御機構" 羊土社, 242 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田沼 靖一: "アポトーシスとは何か-死からはじまる生の科学-" 講談社, 239 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田沼 靖一: "アポトーシスの分子機構「生命のプログラムとアポトーシス」" 日本アクセル・シュプリンガー, 175 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junji.Yamauchi and Sei-ichi Tanuma: "Occurence of an NAD+ glycohydrolase in bovine brain cytosol." Arch.Biochem.Biophys.308. 327-329 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimiko Makino, Masahiko Ikekita, Tamotsu Kondo, Sei-ichi Tanuma and Hiroyuki Ohshima: "Change in electrophoretic mobility of HL-60RG cells by apoptosis." Colloid and Polymer Sci.272. 487-492 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyuki Kuribayashi, Hiroshi Sakagami, Tadashi Sakagami, Ei-ichi Niimi, Daisuke Shiokawa, Masahiro Ikekita, Minoru Takeda and Sei-ichi Tanuma: "Induction of DNA Fragmentation in Human Myelogenous Leukwemic Cell Lines by Sodium 5,6-Benzylidene-L-Ascorbate and its Related Compounds" Anticancer Res.14. 969-976 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma and Daisuke Shiokawa: "Multiple forms of nuclear deoxyribonuclease in rat thymocytes." Biochem Biophys.Res.Commun.203. 3789-3797 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazumasa Aoki, Hideki Nakashima, Toshihiro Hattori, Daisuke Shiokawa, Ei-ichi Ni-imi, Yutaka Tanimoto, Hideharu Maruta, Fumiaki Uchiumi, Mutsuyuki Kochi, Naoki Yamamoto and Sei-ichi Tanuma: "Sodium benzylideneascorbate induced apoptpsis in HIV-replicating UI cells." FEBS Lett.351. 105-108 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Daisuke Shiokawa, Harumi Ohyama, Takeshi Yamada, Kenji Takahashi, and Sei-ichi Tanuma: "Identification of an ensonuclease responsible for apoptosis in rat thymocytes." Eur.J.Biochem.226. 23-30 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shuji Kojima, Hideki I-izuka, Haruo Yamaguchi, Sei-ichi Tanuma, Mutsuyuki Kochi, and Yoshio Ueno.: "Antioxidative activity of sodium benzylideneascorbate and its adoriamycin-induced cardiac toxicity." Anticancer Res. 14. 1875-1880 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazumasa Aoki, Hideharu Maruta, Fumiaki Uchiumi, Tsutomu Hatano, Takashi Yoshida, and Sei-ichi Tanuma: "A Macrocircular ellagitannin, oenothein B,supresses mouse mammary tumor gene expression via inhibition of poly (ADP-ribose) glycohydrolase." Biochem.Biophys.Res.Commun.210. 329-337 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiyuki Kanai, Osamu Takeda, Keiji Miura, Mieko Amagai, Takamasa Kaneko, Tetsuo Kubota, Yukiko Kanai, Sei-ichi Tanuma, and Yoshikazu Kurosawa: "Induction of autoantibodies in normal mice by injection of nucleobindin and natural occurrence of antibodies against nucleobindin in autoimmune MRL/Ipr/Ipr mice." Immunol.Lett.45. 35-42 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: "Apoptosos : a cell ellimination mechanism." Sut.Bull.12. 36-43 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshio Ueno, Kiyoko Umemori, Ei-ich Niimi, Sei-ichi Tanuma, Satoru Nagata, Masao Sugamata, Tomomi Ihara, Masaru Sekijima, Ken-ichi Kawai, Ikuko Ueno, and Fumio Tashiro: "Induction of apoptosis by T2 toxin and other natural toxins in HL-60 human promyelotic leukemia cells." Natural Toxins. 3. 129-137 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: "DNA fragmentation in apoptosis." Tiss.Cult.Res.Commun.14. 133-140 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma, and Daisuke Shiokawa: "An endonuclease responsible for apoptosis." Apoptosis : Prog.Mol.Subcell.Biol.Springer-Verlag. 1-12 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: "Molecular mechanisms of apoptosis." Apoptosis in Normal Development and Cancer (ed.by Mels Sluyser), Taylor & Francis. 39-59 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fumiaki Uchiumi, Hideharu Maruta, Jun-ichiro Inoue, Tadashi Yamamoto, and Sei-ichi Tanuma: "Inhibitory effect of tannic acid on human immunodeficiency virus promoter activity induced by 12-0-tetradecanoylphorbol-13-acetate in Jurkat T-cells." Biochem.Biophys.Res.Commun.220. 411-417 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Daisuke Shiokawa, Kazunari, Nishimura, Hidieharu Maruta, and Sei-ichi Tanuma: "DNA fragmentation during thymic apoptosis is catalyzed by DNase gamma." Apoptosis. 1. 147-152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Abe, and Sei-ichi Tanuma: "Properties of poly (ADP-ribose) glycohydrolase purified from pig testis nuclei." Arch.Biochem.Biophys.336. 139-146 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fumiaki Uchiumi, Tomohiro, Ohta, and Sei-ichi Tanuma: "Replication factor C recognizes 5'-phosphate ends of telomeres." Biochem.Biophys.Res.Commun.229. 310-315 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: Univ Press. Apoptosis-cell survival ordeath, 145 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: Shujunshia. Protdol of apoptosis experiments, 236 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: Science America. Apoptosis-structure and function, 185 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: Yohtoshia. Regulatory mechanisms of apoptosis, 242 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: Kodanshia. What is apoptosis?, 239 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei-ichi Tanuma: Accel springer Japan. Molecular mechanisms of apoptosis, 175 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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