1994 Fiscal Year Annual Research Report

植物器官の代謝発現に関わる遺伝的プログラム

Research Project

Project/Area Number	06278102
Research Institution	Nagoya University
Principal Investigator	中村研三名古屋大学, 農学部, 教授 (80164292)
Co-Investigator(Kenkyū-buntansha)	内藤哲北海道大学, 農学部, 教授 (20164105) 杉山達夫名古屋大学, 農学部, 教授 (50023453) 島本功奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授大山莞爾京都大学, 農学部, 教授 (40135546) 内宮博文東京大学, 分子細胞研究所, 教授 (50142229)
Keywords	植物器官 / 遺伝子発現制御 / 制御遺伝子 / 転写制御因子 / 情報伝達因子 / 遺伝的プログラム / モデル植物
Research Abstract	植物体内を転流する糖のレベルによって種々の器官代謝機能に関わる遺伝子発現が調節されるが、糖応答性遺伝子の5'-上流域にTGGACGG配列よりなる糖応答性エレメントが同定され、この配列への核タンパク質の結合活性は組織の糖処理によって増加することが明らかになった(中村)。糖応答性遺伝子発現にはタンパク質のリン酸化・脱リン酸化、それにCa^<2+>-情報伝達系が関与し、組織への糖供与が細胞内Ca^<2+>濃度の増加をもたらすことが示され(中村)、根や葉での窒素同化に関わる遺伝子の硝酸シグナルによる誘導にもタンパク質のリン酸化・脱リン酸化反応やCa^<2+>情報伝達系が関与することが示唆された(杉山)。トランスジェニックイネにおけるヒストン遺伝子の分裂組織での発現はヘキサマーやG-boxモチーフによって規定され(島本)、これら配列に結合するbZIP型因子の間で複合体形成が起こることが示され、またG-box結合因子には潜在的な転与活性化領域があることが明らかになった(飯)。イネのbZIP型DNA結合因子cDNAの中の一つであるOSBZ8はABA応答性エレメントに特異的に結合するタンパク質をコードし、ABAによる誘導に先だって発現が誘導されることからABA誘導性遺伝子発現にかかわる転写制御因子であることが示唆された(服部)。タバコのnic1,nic2遺伝子はニコチン生合成系遺伝子群の根での発現を制御する遺伝子であることが示され(橋本)、シロイヌナズナのメチオニンを過剰蓄積するmto1-1変異株では母方遺伝子型によって種子貯蔵タンパク質遺伝子の発現が制御されることが明らかになり(内藤)、糖応答性遺伝子発現が抑制された変異株lsi1では葉の正常な成長が糖濃度依存的に異常になった(中村)。トランスジェニック植物において、細胞内アデニンヌクレオチドバランスを調節するアデニレートキナーゼの遺伝子発現レベルを人為的に操作すると成長に顕著な影響が現れた(内宮)。

Research Products
(31 results)

All Other

All Publications (31 results)

[Publications] S.Ishiguro: "Characterization of a cDNA encoding a novel DNA-binding protein,SPF1,that recognizes SP8 sequences in the 5'-upstream regions of genes coding for sporamin and β-amylase from sweet potato." Molecular and General Genetics. 244. 563-571 (1994)
[Publications] A.Ishikawa: "A family of potato genes that encode Kunitz-type proteinase inhibitors:structural somparisons and differential expression." Plant & Cell Physiology. 35. 303-312 (1994)
[Publications] A.Ishikawa: "Structure-activity relationships of jasmonates in the induction of expression of two proteinase inhibitor genes of potato." Bioscience,Biotechnology & Biochemistry. 58. 544-547 (1994)
[Publications] S.Takeda: "Inhibitors of protein phosphatases 1 and 2A block the sugar-inducible gene expression in plants." Plant Physiology. 106. 567-574 (1994)
[Publications] A.Ishikawa: "Jasmonate-inducible expression of a potato cathepsin D inhibitor-GUS gene fusion in tobacco cells." Plant Molecular Biology. 26. 403-414 (1994)
[Publications] S.Mita: "Sugar-inducibloe expression of a gene for β-amylase in Arabidopsis thaliana." Plant Physiology. 107. in press (1995)
[Publications] M.Ohto: "Involvement of Ca^<2+>-signalling in the sugar-inducible expression of genes coding for sporamin and β-amylase of sweet potato." Plant Journal. 7(in press). (1995)
[Publications] S.Takeda: "Spatial patterns of sucrose-inducible and polygalacturonic acid-inducible expression of genes coding for sporamin and β-amylase of sweet potato." Plant & Cell Physiology. 36(in press). (1995)
[Publications] E.T.Aspuria: "Phenotypic instability of Transgenic tobacco plants and their progenies expressing Arabidopsis thaliana small GTP-binding protein genes." Molecular and General Genetics. 248(in press). (1994)
[Publications] T.Anai: "In vitro mutation analysis of Arabidopsis thaliana small GTP-binding proteins and detection of GAP-like activities in plant cells." FEBS Letters. 346. 175-180 (1994)
[Publications] M.Umeda: "Expressed sequence tags from cultured cells of rice(Oryza sativaL.)under stressed conditions:analysis of transcripts of genes engaged in ATP-generating pathways." Plant Molecular Biology. 25. 469-478 (1994)
[Publications] M.Umeda: "Differential transcript level of genes associated with glycolysis and alcohol fermentation in rice plant(Oryza sativaL.)under submergence stress." Plant Physiology. 104. 1015-1022 (1994)
[Publications] N.Fujii: "Analysis of the rolC promoter region involved in somatic embryogenesis-related activation in carrot cell cultures." Plant Physiology. 104. 1151-1157 (1994)
[Publications] S.Kidou: "Isolation and characterization of a rice cDNA similar to S-phase-specific cyc07 gene." Plant Molecular Biology. 24. 545-547 (1994)
[Publications] G.Kochert: "Mapping of expressed sequenced tags loci in rice." Proceedings of the National Academy of Sciences,U.S.A.91(in press). (1994)
[Publications] R.Matsuki: "A 43-kDa nuclear tobacco protein interacts with a specific single-stranded DNA sequence from the 5'-upstream region of the Agrobacterium rhisogenes rolC gene." Gene. 141. 201-205 (1994)
[Publications] R.Yokoyama: "The rolC promoter of Agrogacterium rhizogenes Ri plasmid is activated by sucrose in transgenic tobacco plants." Molecular and General Genetics. 244. 15-22 (1994)
[Publications] M.Kawai: "Tissue specific localization of adenylate kinase in rice (Oryza sativaL.) plants." Journal of Plant Physiology. (in press). (1995)
[Publications] A.Yano: "Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ related alteration of NDK activities during rice seed germination." Plant Molecular Biology. in press (1995)
[Publications] M.Hirai: "Differential regulation of soybean seed storage protein gene promoter-GUS fusions by exogenously applied methionine in transgenic Arabidopsis thaliana." Plant & Cell Physiology. 35. 927-934 (1994)
[Publications] S.Naito: "Maternal effects of mto 1 mutation,that causes overaccumulation of soluble methionine,on the expression of a soybean β-conglycinin gene promoter-GUS fusion in transgenic Arabidopsis thaliana." Plant & Cell Physiology. 35. 1057-1063 (1994)
[Publications] S.Naito: "Expression of soybean seed storage protein genes in transgenic plants and their response to sulfur nutritional conditions." Journal of Plant Physiology. (in press). (1995)
[Publications] E.nambara: "A regulatory role for the ABI3 gene in the establishment of embryo maturation in Arabidopsis thaliana." Development. (in press). (1995)
[Publications] N.Hibi: "Gene expression in tobacco low-nicotine mutants." Plant Cell. 6. 723-735 (1994)
[Publications] T.Hattori: "Sequence and functional analyses of the rice gene homologous to the maize Vp1." Plant Molecular Biology. 24. 805-810 (1994)
[Publications] T.Hattori: "Regulation of the Osem gene by absisic acid and the transcriptional activatior VP1:analysis of cis-acting promoter elements required for regulation by abscisic acid and VP1." Plant Journal. (in press). (1995)
[Publications] G.Hye Huh: "Differential expression of the two types of histone H2A genes in wheat." Biochimica Biophysica Acta. in press. (1995)
[Publications] R.Terada: "A type element composed of the hexamer (ACGTCA) and octamer (CGCGGATC) motifs plays a role(s) in meristematic expression of a wheat histone H3 gene in transgenic rice plants." Plant Molecular Biology. 27. 17-26 (1995)
[Publications] J.Kyozuka: "Promoter elements required for developmental expression of the maize Adh1 gene in transgenic rice." Plant Cell. 6. 799-810 (1994)
[Publications] 中村研三: "ジャスモン酸による植物遺伝子の発現制御" 日本農芸化学会誌. 69(印刷中). (1995)
[Publications] 中村研三: "植物における遺伝子発現調節シグナルとしてのショ糖" バイオサイエンスとインダストリー. (印刷中). (1995)

1994 Fiscal Year Annual Research Report

植物器官の代謝発現に関わる遺伝的プログラム

Principal Investigator

中村 研三 名古屋大学, 農学部, 教授 (80164292)

Research Products

[Publications] S.Ishiguro: "Characterization of a cDNA encoding a novel DNA-binding protein,SPF1,that recognizes SP8 sequences in the 5'-upstream regions of genes coding for sporamin and β-amylase from sweet potato." Molecular and General Genetics. 244. 563-571 (1994)

[Publications] A.Ishikawa: "A family of potato genes that encode Kunitz-type proteinase inhibitors:structural somparisons and differential expression." Plant & Cell Physiology. 35. 303-312 (1994)

[Publications] A.Ishikawa: "Structure-activity relationships of jasmonates in the induction of expression of two proteinase inhibitor genes of potato." Bioscience,Biotechnology & Biochemistry. 58. 544-547 (1994)

[Publications] S.Takeda: "Inhibitors of protein phosphatases 1 and 2A block the sugar-inducible gene expression in plants." Plant Physiology. 106. 567-574 (1994)

[Publications] A.Ishikawa: "Jasmonate-inducible expression of a potato cathepsin D inhibitor-GUS gene fusion in tobacco cells." Plant Molecular Biology. 26. 403-414 (1994)

[Publications] S.Mita: "Sugar-inducibloe expression of a gene for β-amylase in Arabidopsis thaliana." Plant Physiology. 107. in press (1995)

[Publications] M.Ohto: "Involvement of Ca^<2+>-signalling in the sugar-inducible expression of genes coding for sporamin and β-amylase of sweet potato." Plant Journal. 7(in press). (1995)

[Publications] S.Takeda: "Spatial patterns of sucrose-inducible and polygalacturonic acid-inducible expression of genes coding for sporamin and β-amylase of sweet potato." Plant & Cell Physiology. 36(in press). (1995)

[Publications] E.T.Aspuria: "Phenotypic instability of Transgenic tobacco plants and their progenies expressing Arabidopsis thaliana small GTP-binding protein genes." Molecular and General Genetics. 248(in press). (1994)

[Publications] T.Anai: "In vitro mutation analysis of Arabidopsis thaliana small GTP-binding proteins and detection of GAP-like activities in plant cells." FEBS Letters. 346. 175-180 (1994)

[Publications] M.Umeda: "Expressed sequence tags from cultured cells of rice(Oryza sativaL.)under stressed conditions:analysis of transcripts of genes engaged in ATP-generating pathways." Plant Molecular Biology. 25. 469-478 (1994)

[Publications] M.Umeda: "Differential transcript level of genes associated with glycolysis and alcohol fermentation in rice plant(Oryza sativaL.)under submergence stress." Plant Physiology. 104. 1015-1022 (1994)

[Publications] N.Fujii: "Analysis of the rolC promoter region involved in somatic embryogenesis-related activation in carrot cell cultures." Plant Physiology. 104. 1151-1157 (1994)

[Publications] S.Kidou: "Isolation and characterization of a rice cDNA similar to S-phase-specific cyc07 gene." Plant Molecular Biology. 24. 545-547 (1994)

[Publications] G.Kochert: "Mapping of expressed sequenced tags loci in rice." Proceedings of the National Academy of Sciences,U.S.A.91(in press). (1994)

[Publications] R.Matsuki: "A 43-kDa nuclear tobacco protein interacts with a specific single-stranded DNA sequence from the 5'-upstream region of the Agrobacterium rhisogenes rolC gene." Gene. 141. 201-205 (1994)

[Publications] R.Yokoyama: "The rolC promoter of Agrogacterium rhizogenes Ri plasmid is activated by sucrose in transgenic tobacco plants." Molecular and General Genetics. 244. 15-22 (1994)

[Publications] M.Kawai: "Tissue specific localization of adenylate kinase in rice (Oryza sativaL.) plants." Journal of Plant Physiology. (in press). (1995)

[Publications] A.Yano: "Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ related alteration of NDK activities during rice seed germination." Plant Molecular Biology. in press (1995)

[Publications] M.Hirai: "Differential regulation of soybean seed storage protein gene promoter-GUS fusions by exogenously applied methionine in transgenic Arabidopsis thaliana." Plant & Cell Physiology. 35. 927-934 (1994)

[Publications] S.Naito: "Maternal effects of mto 1 mutation,that causes overaccumulation of soluble methionine,on the expression of a soybean β-conglycinin gene promoter-GUS fusion in transgenic Arabidopsis thaliana." Plant & Cell Physiology. 35. 1057-1063 (1994)

[Publications] S.Naito: "Expression of soybean seed storage protein genes in transgenic plants and their response to sulfur nutritional conditions." Journal of Plant Physiology. (in press). (1995)

[Publications] E.nambara: "A regulatory role for the ABI3 gene in the establishment of embryo maturation in Arabidopsis thaliana." Development. (in press). (1995)

[Publications] N.Hibi: "Gene expression in tobacco low-nicotine mutants." Plant Cell. 6. 723-735 (1994)

[Publications] T.Hattori: "Sequence and functional analyses of the rice gene homologous to the maize Vp1." Plant Molecular Biology. 24. 805-810 (1994)

[Publications] T.Hattori: "Regulation of the Osem gene by absisic acid and the transcriptional activatior VP1:analysis of cis-acting promoter elements required for regulation by abscisic acid and VP1." Plant Journal. (in press). (1995)

[Publications] G.Hye Huh: "Differential expression of the two types of histone H2A genes in wheat." Biochimica Biophysica Acta. in press. (1995)

[Publications] R.Terada: "A type element composed of the hexamer (ACGTCA) and octamer (CGCGGATC) motifs plays a role(s) in meristematic expression of a wheat histone H3 gene in transgenic rice plants." Plant Molecular Biology. 27. 17-26 (1995)

[Publications] J.Kyozuka: "Promoter elements required for developmental expression of the maize Adh1 gene in transgenic rice." Plant Cell. 6. 799-810 (1994)

[Publications] 中村研三: "ジャスモン酸による植物遺伝子の発現制御" 日本農芸化学会誌. 69(印刷中). (1995)

[Publications] 中村研三: "植物における遺伝子発現調節シグナルとしてのショ糖" バイオサイエンスとインダストリー. (印刷中). (1995)

中村研三名古屋大学, 農学部, 教授 (80164292)