1996 Fiscal Year Annual Research Report

植物器官の代謝発現に関わる遺伝的プログラム

Research Project

Project/Area Number	06278102
Research Institution	Nagoya University
Principal Investigator	中村研三名古屋大学, 農学部, 教授 (80164292)
Co-Investigator(Kenkyū-buntansha)	杉山達夫名古屋大学, 農学部, 教授 (50023453) 篠崎一雄名古屋大学, 理化学研究所・ライフサイエンス筑波研究センター, 主任研究員 (20124216) 島本功奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10263427) 大山莞爾京都大学, 農学部, 教授 (40135546) 内宮博文東京大学, 分子細胞生物学研究所, 教授 (50142229)
Keywords	植物器官 / 遺伝子発現制御 / 制御遺伝子 / 転写制御因子 / 情報伝達因子 / 遺伝的プログラム / モデル植物
Research Abstract	細胞周期特異的遺伝子発現に関わるタイプ1エレメントのOct配列への結合因子を解析し、B班の廣近班員が単離したLBP1がOct配列に結合することを見出した(飯・廣近(B))。シロイヌナズナから酵母のcdc5を相補するMYB因子を単離してDNA結合配列を同定し、分裂組織で強く発現していることを示した(篠崎)。ABA応答性にかかわる転写活性化因子であるトウモロコシのVp1とシロイヌナズナABI3の間で保存されたC末端領域と相同な配列を持つシロイヌナズナのVAL1/2を単離してそのVp1/ABI3ドメインにDNA結合活性を見出し、Vp1もDNA結合能を持つという新しい可能性を示し、Vp1と相互作用する新規タンパク質をTwo-Hybrid systemで同定した(服部)。シロイヌナズナ遺伝子の糖による発現誘導には、負の制御からの脱抑制が重要であることがシスエレメントの解析や突然変異株における発現の解析から示され、これら負の制御配列への結合活性を検出した。また、タバコ葉の糖で誘導される膜の自己リン酸化タンパク質を約千倍に部分精製し、細胞質膜に強固に結合した特異なカルシウム依存性プロテインキナーゼ(CDPK)であることを示した。(中村)。葉の光合成系遺伝子の転写を制御するサイトカイニンは、根に窒素シグナルを与えることで一過的に合成されるこすことを見出し、葉でサイトカイニンで誘導される遺伝子の一つがバクテリア二成分系のレシーバーと高い相同性をもつタンパク質をコードすることを見出した(杉山)。Ac/Dsによるイネ変異誘発系で単離された開花時期の早くなった変異株のDsとの連鎖解析がF5世代まで進み(島本)、メチオニンを過剰蓄積することで種子タンパク質遺伝子の発現に影響を与えるmoto1は5番染色体末端のメチオニン合成系遺伝子の一つとほぼ同位置にマップされた(内藤)。糖応答性遺伝子発現に異常が見られる変異株では、根の糖濃度に対する形態形成応答においても異常が見られた(中村)。

Research Products
(29 results)

All Other

All Publications (29 results)

[Publications] S.Mita: "Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for β-amylase and of the accumulation of anthocyanin that are inducible by sugars." Plant J.(in press). (1997)
[Publications] A.Katoh: "A cemA homologue essential to CO_2 transport in the cyanobacterium,Synechocystis PCC6803." Proc.Natl.Acad.Sci.USA. 93. 4006-4010 (1996)
[Publications] K.Akashi: "Occurrence of nuclear-encoded tRNA^<lle> in mitochondria of the liverwort,Marchantia polymorpha." Curr.Genet.30. 181-185 (1996)
[Publications] F.Nozue: "Characterization of cDNA encoding for phosphoglucose isomerase of rice (Oryza sativa L.)." DNA Seq.6. 127-135 (1996)
[Publications] T.Ueda: "Characterization and subcellular localization of small GTP-binding protein (Ara-4) from Arabidopsis : conditional expression under control of the promoter of the gene for heat-shock protein HSP81-1." Mol.Gen.Genet.250. 533-539 (1996)
[Publications] K.Shinozaki: "Molecular responses to drought and cold stress." Current Opinion in Biotech.7. 161-167 (1996)
[Publications] T.Katagiri: "Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana" Plant Mol.Biol.30. 647-653 (1996)
[Publications] T.Mizoguchi: "A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch,cold,and water stress in Arabidopsis thaliana." Proc.Natl.Acad.Sci.USA. 93. 765-769 (1996)
[Publications] T.Urao: "Molecular cloning and characterization of a gene that encodes a MYC-related protein in Arabidopsis." Plant Mol.Biol.32. 571-576 (1996)
[Publications] T.Urao: "A transcriptional activation domain of ATMYB2,a drought-inducible Arabidopsis Myb-related protein." Plnat J.10. 1145-1148 (1996)
[Publications] H.Nakagawa: "A rice bZIP protein,designated OSBZ8,is rapidly induced by abscisic acid." Plant J.9. 217-227 (1996)
[Publications] H.Nakagawa: "Seed specific transcription factor VP1 (OSVP1) is expressed in rice suspension-cultured cells." Plant Cell Physiol.37. 355-362 (1996)
[Publications] J.-L.Sun: "Rice embryo globulins : N-terminal amino acid sequences,cDNA cloning and expression." Plant Cell Physiol.37. 612-620 (1996)
[Publications] M.Okanami: "HALF1,a bZIP protein interacting with the wheat transcription factor HBP-1a,contains a novel transcription activation domain." Genes Cell. 1. 87-99 (1996)
[Publications] M.Taniguchi: "Isolation,characterization and expression of cDNA clones encoding a mitochondrial malate translocator from Panicum miliaceum L." Plant Mol.Biol.30. 51-64 (1996)
[Publications] Y.Hihara: "Isolation and characterization of two cDNA clones for mRNAs that are abundantly expressed in immature anthers of rice (Oryza sativa L.)." Plant Mol.Biol.30. 1181-1193 (1996)
[Publications] K.Suehara: "Optimal expression of GUS gene from methyl jasmonate-inducible promoter in high density culture of transformed tobacco cell line BY-2." J.Ferment.Bioeng.82. 51-55 (1996)
[Publications] Y.Yoshioka: "Transient gene expression in plant cells mediated by Agrobacterium tumefaciens : application for the analysis of virulence loci." Plant Cell Physiol.37. 782-789 (1996)
[Publications] T.Hirayama: "A cdc5+ homolong of a higher plant,Arabidopsis thaliana." Proc.Natl.Acad.Sci.USA. 93. 13371-13376 (1996)
[Publications] T.Mizoguchi: "Environmental stress response in plants : the role of mitogen-activated protein kinases (MAPKs)." Trends Biotech.5. 15-19 (1996)
[Publications] S.Oba: "Novel DNA probes capable of discriminating Indica and Japonica rice cultivars." DNA Seq.6. 303-306 (1996)
[Publications] T.Tsuge: "Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh." Development. 122. 1589-1600 (1996)
[Publications] T.Ueda: "The Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase." Plant Cell. 8. 2079-2091 (1996)
[Publications] G.H.Huh: "Structural characteristics of two wheat histone H2A genes encoding distinct types of variants and functional differences in their promoter activity." Plant Mol.Biol.(in press). (1997)
[Publications] T.Nakayama: "Dissection of the wheat transcription factor HBP-1a(17) revealed the modular structure of the activation domain." Mol.Gen.Genet.(in press). (1997)
[Publications] N.Ohtsubo: "Cooperation of two distinct cis-acting elements is necessary for the S-phase-specific activation of the wheat histone H3 promotor." Plant J.(in press). (1997)
[Publications] T.Izawa: "Becoming a model plant : the importance of rice to plant science." Trends Plant Sci.1. 95-99 (1996)
[Publications] A.Ono: "The rab 16B promoter of rice contains two distinct abscisic acid-responsive elements." Plant Physiol.112. 483-491 (1996)
[Publications] T.Izawa: "Transposon tagging in rice" Plant Mol.Biol.(in press). (1997)

1996 Fiscal Year Annual Research Report

植物器官の代謝発現に関わる遺伝的プログラム

Principal Investigator

中村 研三 名古屋大学, 農学部, 教授 (80164292)

Research Products

[Publications] S.Mita: "Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for β-amylase and of the accumulation of anthocyanin that are inducible by sugars." Plant J.(in press). (1997)

[Publications] A.Katoh: "A cemA homologue essential to CO_2 transport in the cyanobacterium,Synechocystis PCC6803." Proc.Natl.Acad.Sci.USA. 93. 4006-4010 (1996)

[Publications] K.Akashi: "Occurrence of nuclear-encoded tRNA^<lle> in mitochondria of the liverwort,Marchantia polymorpha." Curr.Genet.30. 181-185 (1996)

[Publications] F.Nozue: "Characterization of cDNA encoding for phosphoglucose isomerase of rice (Oryza sativa L.)." DNA Seq.6. 127-135 (1996)

[Publications] T.Ueda: "Characterization and subcellular localization of small GTP-binding protein (Ara-4) from Arabidopsis : conditional expression under control of the promoter of the gene for heat-shock protein HSP81-1." Mol.Gen.Genet.250. 533-539 (1996)

[Publications] K.Shinozaki: "Molecular responses to drought and cold stress." Current Opinion in Biotech.7. 161-167 (1996)

[Publications] T.Katagiri: "Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana" Plant Mol.Biol.30. 647-653 (1996)

[Publications] T.Urao: "Molecular cloning and characterization of a gene that encodes a MYC-related protein in Arabidopsis." Plant Mol.Biol.32. 571-576 (1996)

[Publications] T.Urao: "A transcriptional activation domain of ATMYB2,a drought-inducible Arabidopsis Myb-related protein." Plnat J.10. 1145-1148 (1996)

[Publications] H.Nakagawa: "A rice bZIP protein,designated OSBZ8,is rapidly induced by abscisic acid." Plant J.9. 217-227 (1996)

[Publications] H.Nakagawa: "Seed specific transcription factor VP1 (OSVP1) is expressed in rice suspension-cultured cells." Plant Cell Physiol.37. 355-362 (1996)

[Publications] J.-L.Sun: "Rice embryo globulins : N-terminal amino acid sequences,cDNA cloning and expression." Plant Cell Physiol.37. 612-620 (1996)

[Publications] M.Okanami: "HALF1,a bZIP protein interacting with the wheat transcription factor HBP-1a,contains a novel transcription activation domain." Genes Cell. 1. 87-99 (1996)

[Publications] M.Taniguchi: "Isolation,characterization and expression of cDNA clones encoding a mitochondrial malate translocator from Panicum miliaceum L." Plant Mol.Biol.30. 51-64 (1996)

[Publications] Y.Hihara: "Isolation and characterization of two cDNA clones for mRNAs that are abundantly expressed in immature anthers of rice (Oryza sativa L.)." Plant Mol.Biol.30. 1181-1193 (1996)

[Publications] K.Suehara: "Optimal expression of GUS gene from methyl jasmonate-inducible promoter in high density culture of transformed tobacco cell line BY-2." J.Ferment.Bioeng.82. 51-55 (1996)

[Publications] Y.Yoshioka: "Transient gene expression in plant cells mediated by Agrobacterium tumefaciens : application for the analysis of virulence loci." Plant Cell Physiol.37. 782-789 (1996)

[Publications] T.Hirayama: "A cdc5+ homolong of a higher plant,Arabidopsis thaliana." Proc.Natl.Acad.Sci.USA. 93. 13371-13376 (1996)

[Publications] T.Mizoguchi: "Environmental stress response in plants : the role of mitogen-activated protein kinases (MAPKs)." Trends Biotech.5. 15-19 (1996)

[Publications] S.Oba: "Novel DNA probes capable of discriminating Indica and Japonica rice cultivars." DNA Seq.6. 303-306 (1996)

[Publications] T.Tsuge: "Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh." Development. 122. 1589-1600 (1996)

[Publications] T.Ueda: "The Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase." Plant Cell. 8. 2079-2091 (1996)

[Publications] G.H.Huh: "Structural characteristics of two wheat histone H2A genes encoding distinct types of variants and functional differences in their promoter activity." Plant Mol.Biol.(in press). (1997)

[Publications] T.Nakayama: "Dissection of the wheat transcription factor HBP-1a(17) revealed the modular structure of the activation domain." Mol.Gen.Genet.(in press). (1997)

[Publications] N.Ohtsubo: "Cooperation of two distinct cis-acting elements is necessary for the S-phase-specific activation of the wheat histone H3 promotor." Plant J.(in press). (1997)

[Publications] T.Izawa: "Becoming a model plant : the importance of rice to plant science." Trends Plant Sci.1. 95-99 (1996)

[Publications] A.Ono: "The rab 16B promoter of rice contains two distinct abscisic acid-responsive elements." Plant Physiol.112. 483-491 (1996)

[Publications] T.Izawa: "Transposon tagging in rice" Plant Mol.Biol.(in press). (1997)

中村研三名古屋大学, 農学部, 教授 (80164292)