1994 Fiscal Year Annual Research Report

植物器官の形態形成に関わる遺伝的プログラム

Research Project

Project/Area Number	06278103
Research Institution	Okazaki National Research Institutes
Principal Investigator	岡田清孝岡崎国立共同研究機構, 基礎生物学研究所, 助教授 (50101093)
Co-Investigator(Kenkyū-buntansha)	福田裕穂東北大学, 理学部, 教授 (10165293) 広近洋彦農業生物資源研究所, 分子育種部, 室長長戸泰郎東京大学, 農学部, 助教授 (10143413) 後藤弘爾京都大学, 化学研究所, 助手 (00251489) 飯田滋東京理科大学, 基礎工学部, 教授 (30012777) 町田泰則名古屋大学, 理学部, 教授 (80175596)
Keywords	分子生物学 / 分子遺伝学 / シロイヌナズナ / イネ / トランスジェニック植物 / トランスポゾン / 器官発生 / 形態形成
Research Abstract	研究実績の概要シロイヌナズナおよびイネから器官の形態異常および発生異常突然変異株を多数分離し詳細な遺伝解析をおこなって遺伝子座を決定するとともに、多重突然変異体の形質を調べることによって機能発現の順位や相互作用の可能性について検討した。岡田は、花芽分裂組織形成と維持に関わる遺伝子の突然変異体を解析した。花茎分裂組織から花芽分裂組織の形成過程を制御する遺伝子FIL,PIN-FORMEDについて検討し、これらの遺伝子産物がLFY,AP1,CALなどと相互作用していることを示した。後藤は、トランスジェニック植物を作製してBグループのホメオティック遺伝子の発現領域を解析し、AP3とPIが異なった発現領域制御機構の支配を受けていることをあきらかにした。長戸は多数のイネ胚発生異常突然変異体を調べ、胚乳の発達を制御する遺伝子の異常が胚のサイズを決定していることを明らかにした。一方、町田はAcトランスポゾンを介したゲノム欠失・遺伝子タッギング法を開発した。現在、いくつかの形態異常突然変異体を得て解析している。岡田は遺伝子タッギングによって胚軸と根の成長および屈性を支配するHY5遺伝子を分離してその構造と発現を解析した。HY5は若い植物のシグナル伝達機構の中心的な機能を持つ遺伝子の一つで多くの研究者が単離を競っていたものである。福田は、維管束形成を制御するTED2およびTED3遺伝子を単離し、発現パターンを詳細に調べた。飯田はアサガオ花弁のキメラ斑がトランスポゾンによって生じることをあきらかにし、変異誘発遺伝子の存在を示した。広近はタバコのレトロトランスポゾンの転写調節領域に変異を導入し、トランスジェニック・タバコを作成してサリチル酸応答に関わるシスエレメントを解析し、さらにトランス因子を単離して構造を明らかにした。

Research Products
(20 results)

All Other

All Publications (20 results)

[Publications] H.Okamoto,H.Shiraishi,K.Okada,Y.Shimura: "Isolation and charactreization of ADA1,and Arabidopsis homolog of deficiens gene of Antirrhinum majus" Plant Mol.Biol.26. 465-472 (1994)
[Publications] T.Ito,H.Shiraishi,K.Okada,Y.Shimura: "Two amidophosphoribosyltransferase genes of Arabidopsis thaliana" Plant Mol.Biol.26. 529-533 (1994)
[Publications] K.Okada,Y.Shimura: "Genetic analysis of signaling in flower development using Arabidopsis21GC03:Plant Mol.Biol." 26. 1357-1377 (1994)
[Publications] Muranaka,T.,Banno,H.,Machida,Y.: "Characterization of tobacco protein kinase NPK5,a homologue of yeast SNF1,that constitutively activates exxpression of the glucose-repressible SUC2 gene for a secreted of yeast" Mol.Cell.Biol.14. 2958-2965 (1994)
[Publications] Muranaka,T.,Niwa,Y.,Machida,Y.: "A model for a bioconversion system with the promoter of the parAt gene,which confers a high level of expressionof a transgene in hairy roots." Appl.Microbiol.Biotechnol.40. 841-845 (1994)
[Publications] Ito,Y.,Moribe,T.,Banno,H.,Machida,Y.: "NPK15,a tobacco protein-serine/threonine kinase with a single hydrophobic region near the amino terminus" Mol.Gen.Genet.245. 1-10 (1994)
[Publications] Ito,M.,Sato,T.,Fukuda,H.,Komamine,A.: "Meristem-specific gene expression directed by the promoter of the S-phase-specific gene,cyc07" Plant Mol.Biol.24. 863-878 (1994)
[Publications] Fukuda,H.,Ito,M.,Sugiyama,M.,Komamine,A.: "Mechanisms of the proliferation and differentiation of palnt cells in cell culture system" Int.J.Develop.Biol.38. 287-299 (1994)
[Publications] Kobayashi,H.,Fukuda,H.: "Involvement of Calmodulin and Calmodulin-binding proteins in the differentiation oftracheary elements in Zinnia cells" Planta. 194. 388-394 (1994)
[Publications] Fukuda,H.: "Redifferentiation of single mesophyll cells into trracheary elements" Int.J.Plant Sci. 155. 262-271 (1994)
[Publications] Sugiyama,M.,Fukuda,H.,Komamine,A.: "Characteristics of the inhibitory effects of aphidicolin on transdiffierentiation into tracheary elements of isolated mesophyll cells of Zinnia elegans" Plant Cell Physiol.35. 519-522 (1194)
[Publications] Kawahara,R.,Sunabori,S.,Fukuda,H.,Komamine,A.: "Analysis by in situ hybridization of the expression of elongation factor 1 in the carrotcells during somatic embryogenesis" J.Plant Res. 107. 361-364 (1994)
[Publications] Demura,T.,Fukuda,H.: "Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development" Plant cell. 6. 967-981 (1994)
[Publications] Y.Inagaki,Y.Hisatomi,T.Suzuki,K.Kasahara,S.Iida: "Isolation of a Supperssor-mutator/Enhahcer-like transposable element,Tpn1,from Japanese mornong glory bearing variegated flowers" Plant Cell. 6. 375-383 (1994)
[Publications] H.Hoshino,Y.Inagaki,S.Iida: "Structural analysis of Tnp1,a transposable element from Japanese mornong glory bearing variegated flowers" Mol.Gen.Genet.
[Publications] Goto,K.,E.M.Meyerowitz: "Function and regulation of the Arabidopsis fliral homeotic gene PISTLLATA" Genes Dev.8. 1548-1560 (1994)
[Publications] Hong,S.K.,Aoki,T.,Kitano,H.,Satoh,H.,Nagata,Y.: "Phenotypic diversity of 188 rice embryo mutants" Devel.Genet.(1995)
[Publications] Sugimoto,K.,Y,Otsuki S.Saji,H.Hirochika: "Transposition of the maize Ds element from a viral vector to the rice genome" Plant J.5. 863-871 (1994)
[Publications] K.Okada,Y.Shimura: "Modulation of root growth by physical stimuli" Cold Spring Harbor Laboratory Press,
[Publications] Sugiyama,M.,Fukuda,H.: "Zinnia mesophyll culture system to study xylogenesis" Kluwer Academic Press, (1995)

1994 Fiscal Year Annual Research Report

植物器官の形態形成に関わる遺伝的プログラム

Principal Investigator

岡田 清孝 岡崎国立共同研究機構, 基礎生物学研究所, 助教授 (50101093)

Research Products

[Publications] H.Okamoto,H.Shiraishi,K.Okada,Y.Shimura: "Isolation and charactreization of ADA1,and Arabidopsis homolog of deficiens gene of Antirrhinum majus" Plant Mol.Biol.26. 465-472 (1994)

[Publications] T.Ito,H.Shiraishi,K.Okada,Y.Shimura: "Two amidophosphoribosyltransferase genes of Arabidopsis thaliana" Plant Mol.Biol.26. 529-533 (1994)

[Publications] K.Okada,Y.Shimura: "Genetic analysis of signaling in flower development using Arabidopsis21GC03:Plant Mol.Biol." 26. 1357-1377 (1994)

[Publications] Muranaka,T.,Banno,H.,Machida,Y.: "Characterization of tobacco protein kinase NPK5,a homologue of yeast SNF1,that constitutively activates exxpression of the glucose-repressible SUC2 gene for a secreted of yeast" Mol.Cell.Biol.14. 2958-2965 (1994)

[Publications] Muranaka,T.,Niwa,Y.,Machida,Y.: "A model for a bioconversion system with the promoter of the parAt gene,which confers a high level of expressionof a transgene in hairy roots." Appl.Microbiol.Biotechnol.40. 841-845 (1994)

[Publications] Ito,Y.,Moribe,T.,Banno,H.,Machida,Y.: "NPK15,a tobacco protein-serine/threonine kinase with a single hydrophobic region near the amino terminus" Mol.Gen.Genet.245. 1-10 (1994)

[Publications] Ito,M.,Sato,T.,Fukuda,H.,Komamine,A.: "Meristem-specific gene expression directed by the promoter of the S-phase-specific gene,cyc07" Plant Mol.Biol.24. 863-878 (1994)

[Publications] Fukuda,H.,Ito,M.,Sugiyama,M.,Komamine,A.: "Mechanisms of the proliferation and differentiation of palnt cells in cell culture system" Int.J.Develop.Biol.38. 287-299 (1994)

[Publications] Kobayashi,H.,Fukuda,H.: "Involvement of Calmodulin and Calmodulin-binding proteins in the differentiation oftracheary elements in Zinnia cells" Planta. 194. 388-394 (1994)

[Publications] Fukuda,H.: "Redifferentiation of single mesophyll cells into trracheary elements" Int.J.Plant Sci. 155. 262-271 (1994)

[Publications] Sugiyama,M.,Fukuda,H.,Komamine,A.: "Characteristics of the inhibitory effects of aphidicolin on transdiffierentiation into tracheary elements of isolated mesophyll cells of Zinnia elegans" Plant Cell Physiol.35. 519-522 (1194)

[Publications] Kawahara,R.,Sunabori,S.,Fukuda,H.,Komamine,A.: "Analysis by in situ hybridization of the expression of elongation factor 1 in the carrotcells during somatic embryogenesis" J.Plant Res. 107. 361-364 (1994)

[Publications] Demura,T.,Fukuda,H.: "Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development" Plant cell. 6. 967-981 (1994)

[Publications] Y.Inagaki,Y.Hisatomi,T.Suzuki,K.Kasahara,S.Iida: "Isolation of a Supperssor-mutator/Enhahcer-like transposable element,Tpn1,from Japanese mornong glory bearing variegated flowers" Plant Cell. 6. 375-383 (1994)

[Publications] H.Hoshino,Y.Inagaki,S.Iida: "Structural analysis of Tnp1,a transposable element from Japanese mornong glory bearing variegated flowers" Mol.Gen.Genet.

[Publications] Goto,K.,E.M.Meyerowitz: "Function and regulation of the Arabidopsis fliral homeotic gene PISTLLATA" Genes Dev.8. 1548-1560 (1994)

[Publications] Hong,S.K.,Aoki,T.,Kitano,H.,Satoh,H.,Nagata,Y.: "Phenotypic diversity of 188 rice embryo mutants" Devel.Genet.(1995)

[Publications] Sugimoto,K.,Y,Otsuki S.Saji,H.Hirochika: "Transposition of the maize Ds element from a viral vector to the rice genome" Plant J.5. 863-871 (1994)

[Publications] K.Okada,Y.Shimura: "Modulation of root growth by physical stimuli" Cold Spring Harbor Laboratory Press,

[Publications] Sugiyama,M.,Fukuda,H.: "Zinnia mesophyll culture system to study xylogenesis" Kluwer Academic Press, (1995)

岡田清孝岡崎国立共同研究機構, 基礎生物学研究所, 助教授 (50101093)