1994 Fiscal Year Annual Research Report

HTLV-IおよびHTLV-IIキャリアの遺伝背景の比較研究

Research Project

Project/Area Number	06280229
Research Institution	Kagoshima University
Principal Investigator	藤吉利信鹿児島大学, 医学部, 助教授 (50173480)
Co-Investigator(Kenkyū-buntansha)	屋敷伸治鹿児島大学, 医学部, 助手 (40182315) 園田俊郎鹿児島大学, 医学部, 教授 (40036463)
Keywords	HTLV-I / HTLV-II / HTLV-I / IIキャリア / HLA / 遺伝背景 / 免疫応答
Research Abstract	本研究では、鹿児島県地方のHTLV-Iキャリア、ATLおよびHAM/TSP患者、ならびに南米先住民のHTLV-I/IIキャリアから、血清・リンパ球を収集し5000検体をこえる血清・リンパ球バンクを樹立した。この検体を用いて、HTLV-I/IIキャリアのHLAハプロタイプをPCR-RFLP法-DNAタイピングにより調べたところ、(1)鹿児島県地方のATL患者に多くみられるHLAハプロタイプが南米アンデス高地の先住民のHTLV-Iキャリアにも多くみられること、(2)南米オリノコ川流域のHTLV-IIキャリアのHLAハプロタイプはアンデス高地先住民のHTLV-Iキャリアとは異なることが明かとなった(研究発表・雑誌論文1、2)。このことから、南米先住民のHTLV-Iキャリアの民族の起源がユーラシア大陸のモンゴロイド起源であることが示唆された。さらに、HTLV-I感染細胞に対するCTLを、ATL、HTLV-Iキャリアの末梢血リンパ球からin vitroで誘導する方法を開発し、その性状を調べたところ、その宿主のHLAハプロタイプにより標的となる抗原に特徴がみられた(研究発表・雑誌論文5)。また、ATL、HAM/TSPにおけるHTLV-Iウイルスの発現とウイルス量の変動を明らかにした(研究発表・雑誌論文3、4)。本研究で樹立したHTLV-I/IIキャリアの血清バンクは、HTLV-I/II抗体検査法(PHA、ELISA、WB)の特異性および感度を評価する標準血清として有用であり、WHOの委託研究に用いて成果をあげた(研究発表・雑誌論文7、8)。

Research Products

(10 results)

All Other

All Publications (10 results)

[Publications] 1.Fujiyoshi T.,et al.: "Genetic segregation of HTLV-I and HTLV-II carriers among South American natives." AIDS.Res.Human Retroviruses.10. 486- (1994)
[Publications] 2.Zaninovic V.,et al.: "Geographic independence of HTLV-I and HTLV-II foci in the Andes high land,the Atlantic coast and Orinoco of Colombia." AIDS.Res.Human Retroviruses.10. 97-101 (1994)
[Publications] 3.Setoyama M.,Fujiyoshi T.,et al.: "HTLV-I messenger RNA is expressed in adult T-cell leukemia/lymphoma patients in vivo:An in situ hybridization study." Int.J.Cancer.57. 760-764 (1994)
[Publications] 4.Kubota R.,Fujiyoshi T.,et al.: "HTLV-I proviral DNA amount correlates with infiltrating CD4+ lymphocytes in the spinal cord from patients with HTLV-I-associated mvclopathy." J.Neuroimmunol.53. 23-29 (1994)
[Publications] 5.Katahira Y.,Fujiyoshi T.,et al.: "In vitro induction of cytotoxic T lymphocytes against HTLV-I-infected T-cells from ATL patients,asymtomatic HTLV-I carriers and serenegative healthy donors." Jpn.J.Cancer Res.86. 21-27 (1995)
[Publications] 6.Blank M.,et al.: "Distribution of HLA antigens and heplotypes of Colombian and Jamaican black populations." Tissue Antigens.45. 111-116 (1995)
[Publications] 7.Fujiyama C.,Fujiyoshi T.,et al.: "Evaluation of commercial anti-HTLV-I testing kits by the standard HTLV-I panel sera." WHO Bullitein.(in press). (1995)
[Publications] 8.Fujiyama C.,Fujiyoshi T.,et al.: "Re-evaluation of anti-HTLV-I western blot assay by the standard HTLV-I and HTLV-II panel sera." Clinical Diagnostic Virology. (in press). (1995)
[Publications] 9.Miyashita H.,Fujiyoshi T.,et al.: "Cloning of HLA-A26 cDNA from Japanese donors possessing of "ATL-associated HLA haplotypes"" Tissue Antigens.(in press). (1995)
[Publications] 10.Ushikai M.,Fujiyoshi T.,et al.: "Detection and cloning of human papillomavirus DNA associated with recurrent respiratory papillomatosis in Thailand." Jpn.J.Cancer Res.85. 699-703 (1994)

1994 Fiscal Year Annual Research Report

HTLV-IおよびHTLV-IIキャリアの遺伝背景の比較研究

Principal Investigator

藤吉 利信 鹿児島大学, 医学部, 助教授 (50173480)

Research Products

[Publications] 1.Fujiyoshi T.,et al.: "Genetic segregation of HTLV-I and HTLV-II carriers among South American natives." AIDS.Res.Human Retroviruses.10. 486- (1994)

[Publications] 2.Zaninovic V.,et al.: "Geographic independence of HTLV-I and HTLV-II foci in the Andes high land,the Atlantic coast and Orinoco of Colombia." AIDS.Res.Human Retroviruses.10. 97-101 (1994)

[Publications] 3.Setoyama M.,Fujiyoshi T.,et al.: "HTLV-I messenger RNA is expressed in adult T-cell leukemia/lymphoma patients in vivo:An in situ hybridization study." Int.J.Cancer.57. 760-764 (1994)

[Publications] 4.Kubota R.,Fujiyoshi T.,et al.: "HTLV-I proviral DNA amount correlates with infiltrating CD4+ lymphocytes in the spinal cord from patients with HTLV-I-associated mvclopathy." J.Neuroimmunol.53. 23-29 (1994)

[Publications] 5.Katahira Y.,Fujiyoshi T.,et al.: "In vitro induction of cytotoxic T lymphocytes against HTLV-I-infected T-cells from ATL patients,asymtomatic HTLV-I carriers and serenegative healthy donors." Jpn.J.Cancer Res.86. 21-27 (1995)

[Publications] 6.Blank M.,et al.: "Distribution of HLA antigens and heplotypes of Colombian and Jamaican black populations." Tissue Antigens.45. 111-116 (1995)

[Publications] 7.Fujiyama C.,Fujiyoshi T.,et al.: "Evaluation of commercial anti-HTLV-I testing kits by the standard HTLV-I panel sera." WHO Bullitein.(in press). (1995)

[Publications] 8.Fujiyama C.,Fujiyoshi T.,et al.: "Re-evaluation of anti-HTLV-I western blot assay by the standard HTLV-I and HTLV-II panel sera." Clinical Diagnostic Virology. (in press). (1995)

[Publications] 9.Miyashita H.,Fujiyoshi T.,et al.: "Cloning of HLA-A26 cDNA from Japanese donors possessing of "ATL-associated HLA haplotypes"" Tissue Antigens.(in press). (1995)

[Publications] 10.Ushikai M.,Fujiyoshi T.,et al.: "Detection and cloning of human papillomavirus DNA associated with recurrent respiratory papillomatosis in Thailand." Jpn.J.Cancer Res.85. 699-703 (1994)

藤吉利信鹿児島大学, 医学部, 助教授 (50173480)