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1996 Fiscal Year Final Research Report Summary

Isolation and transfer of genes of key enzymes to improve the quality of fruit

Research Project

Project/Area Number 06304013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field 園芸・造園学
Research InstitutionNagoya University

Principal Investigator

YAMAKI Shohei  Nagoya University, Agricultural Sciences, Professor, 農学部, 教授 (70210341)

Co-Investigator(Kenkyū-buntansha) NITO Nobumasa  Saga University, Agriculture, Professor, 農学部, 教授 (80015809)
SUGIURA Akira  Kyoto University, Agriculture, Professor, 農学部, 教授 (00026379)
MII Masahiro  Chiba University, Horticulture, Professor, 園芸学部, 教授 (30093074)
MINAMIKAWA Takao  Tokyo Metoro.University, Science, Professor, 理学部, 教授 (30087001)
AKIHAMA Tomoya  Meiji University, Agriculture, Professor, 農学部, 教授 (90167841)
Project Period (FY) 1994 – 1996
KeywordsACCsynthase cDNA / Cysteine protease gene / Sucrose phosphate synthase cDNA / Sorbitol dehydrogenase cDNA / S6PDH transgenic apple / Transgenic grape / Transgenic citrus / Transgenic persimmon
Research Abstract

This project was conducted by 2 groups. The first group tried the purification and cloning of some enzymes which concern closely the improvement of fruit quality. The base sequence coding cDNA of sorbitol dehydrogenase which converts sorbitol of translocating sugar in Rosaceae fruit to fructose was determined in apple fruit. In tomato fruit, cDNAs of 6 isozymes of ACCsynthase were isolated and the expressions were investigated using the specific antibody for each isozyme. From melon fruit, the cDNA encoding ACCsynthase and ACCoxidase were isolated, the base sequences were determined and the gene expression were investigated with fruit development. The base sequence of cDNA encoding sucrose phosphate synthase, sucrose synthase and acid invertase concverning the biosynthesis and degradation of sucrose of translocating sugar in Citrus were determined. The gene encoding cysteine protease which functions the maturation and senscence was isolated from French bean seed and Vigna mungo seedling, and the structure of promotor and the gene expression were investigated. alpha-Arabinofurano sidase concerning the softening of Japanese pear fruit was purified.
The second group tried to establish the plant-regeneration system and gene transfer method to for the transgenic fruit tree. In apples, the plant-regeneration from the callus of apple cultivar and the gene transfer via Agrobacterium became possible, and we succeeded the transfer of S6PDH chimeric gene. In persimmon, the plant was regenerated from callus and protoplast, and the recombinant of marker gene was formed through Agrobacterium infection. Further, in grape and citurs belonging sub-family of Rutaceae, we succeeded to get the embryogenic callus and to form the recombinant of GUS gene by co-culturing the callus with Agrobacterium. As described above, we could collect some genes concerning closely the fruit quality, and establish the plant-regeneration method and the gene trans fer method in main fruits crops in Japan.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Jumin, H. B. & N. Nito.: "Plant regeneration via somatic embryogenesis from protoplasts of six Plant species related to Citrus." Plant Cell Reports. 15. 332-336 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jumin, H. B. Nito.: "Somatic embryogenesis and plant regeneration from protoplast of Eremocitrus glauca (Lindl.) swing." Phytomorphology. 46. 197-206 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komatsu, A., Y. Takanokura, M. Omura & T. Akihama.: "Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase isoforms from citrus (Citrus unshiu Marc.)." Mol. Gen. Gent.252. 346-351 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miki, t., M. Yamamoto, H. Nakagawa, N. Ogura, H. Mori, H. Imaseki & T. Sato.: "Nucleotide sequence of a cDNA for 1-aminocyclopropane-1-carboxylate synthase from melon fruits." Plant Physiol.107. 297-298 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakano, M., Y. Hoshino & M. Mii.: "Regeneration of transgenic plants of grapevine (Vitus vinifera L.) via Agrobacterium rhizogenes mediated transformation of embryogenic calli." J. Exp. Bot. 45 :. 45. 649-656 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura, M., R. Tao & A. Sugiura.: "Regeneration of somatic hydrids from electrofused protoplasts of Japanese persimmon (Diospyros kaki L.)." Plant Science. 108. 101-107 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tao, R., K. Ozawa, M.Tamura & A. Sugiura.: "Dodecaploid plant regeneration from endosperm culture of persimmon (Diospyros kaki L.)" Acta Hort.436. 119-128 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateishi, A., Y. Kanayama & S. Yamaki.: "α-L-Arabinofuranosidase from cell walls of Japanese pear fruits." Phytochemistry. 42. 295-299 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terasaki, Y., D. Yamauchi, H. Morikawa & T. Minamikawa.: "Influence of axis removal on the expression of a gene for a cysteine endopeptidase (EP-Cl) in french bean cotyledons during germination." Plant Cell Physiol.36. 537-541 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi, H., Y. Kanayama, J. Soejima & S. Yamaki.: "Changes in the amounts of the NAD-dependent sorbitol dehydrogenase and its involvement in the development of apple fruit." J. Amer. Soc. Hort. Sci.121. 848-852 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaki, S.: "Physiology and metabolism of fruit development-Biochemistry of sugar metabolism and compartmentation in fruits." Acta Hort.398. 109-120 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, M., T. Ishiki, K. Fujinami, y. Yanagisawa, H. Nakagawa, N. Ogura, T. Hirabayashi & T. Sato.: "The synthesis of ethylene in melon fruit during the early stage of ripening." Plant Cell Physiol.36. 591-596 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamauchi, D., Y. Terasaki, T. Okamoto and T. Minamikawa.: "Promoter regions of cysteine endopeptidase genes from legumes confer germination-specific expression in transgenic tobacco seeds." Plant Mol. Biol.30. 321-329 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhu, Y.-M., Y.Hoshino, M.Nakano, S.Takahashi & M. Mii.: "Highly efficient system of plant regeneration from protoplasts of grapevine though somatic embryogenesis by using embryogenic callus culture and activated charcoal." Plant Sci.123. 151-157 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jumin, H.B.and N.Nito: "Plant regeneration via somatic embryogenesis from protoplasts of six plant species related to Citrus." Plant Cell Reports. 15. 332-336 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jumin, H.B.and N.Nito: "Somatic embryogenesis and plant regeneration from protoplasts of Eremocitrus glauca (Lindl.) swing." Phytomorphology. 46. 197-206 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komatsu, A., Y.Takanokura, M.Omura and T.Akihama: "Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase isoforms from citrus (Citrus unshiu Marc.)" Mol.Gen.Gent.252. 346-351 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miki, t., M.Yamamoto, H.Nakagawa, N.Ogura, H.Mori, H.Imaseki and T.Sato: "Nucleotide sequence of a cDNA for 1-aminocyclopropane-1-carboxylat synthase from melon fruits." Plant Physiol.107. 297-298 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano, M., Y.Hoshino and M.Mii: "Regeneration of transgenic plants of grapevine (Vitus vinifera L.) via Agrobacterium rhizogenes mediated transformation of embryogenic calli." J.Exp.Bot.45. 649-656 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura, M., R.Tao and A.Sugiura: "Regeneration of somatic hydrids from electrofused protoplasts of Japanese persimmon (Diospyros kaki L.)." Plant Science. 108. 101-107 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tao, R., K.Ozawa, M.Tamura and A.Sugiura: "Dodecaploid plant regeneration from endosperm culture of persimmon (Diospyros kaki L.)" Acta Hort.436. 119-128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateishi, A., Y.Kanayama and S.Yamaki: "alpha-L-Arabinofuranosidase from cell walls of Japanese pear fruits." Phytochem.42. 295-299 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terasaki, Y., D.Yamauchi, H.Morikawa and T.Minamikawa: "Influence of axis removal on the expression of a gene for a cysteine endopeptidase (EP-C1) in french bean cotyledons during germination." Plant Cell Physiol.36. 537-541 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, H., Y.Kanayama, J.Soejima and S.Yamaki: "Changes in the amounts of the NAD-dependent sorbitol dehydrogenase and its involvement in the development of apple fruit." J.Amer.Soc.Hort.Sci.121. 848-852 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaki, S.: "Physiology and metabolism of fruit development-Biochemistry of sugar metabolism and compartmentation in fruits." Acta Hort.398. 109-120 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, M., T.Miki, Y.Ishiki, K.Fujinami, Y.Yanagisawa, H.Nakagawa, N.Ogura, T.Hirabayashi and T.Sato: "The synthesis of ethylene in melon fruit during the early stage of ripening." Plant Cell Physiol.36. 591-596 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamauchi, D., Y.Terasaki, T.Okamoto and T.Minamikawa: "Promoter regions of cysteine endopeptidase genes from legumes confer germinatin-specific expression in transgenic tobacco seeds." Plant Mol.Biol.30. 321-329 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhu, Y.-M., Y.Hoshino, M.Nakano, E.Takahashi and M.Mii: "Highly efficient system of plant regeneration from protoplasts of grapevine (Vitis vinifera L.) through somatic embryogenesis by using embryogenic callus culture and activated charcoal." Plant Sci.123. 151-157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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