1994 Fiscal Year Annual Research Report

植物における細胞間認識と情報伝達

Research Project

Project/Area Number	06304023
Research Institution	Nagoya University
Principal Investigator	道家紀志名古屋大学, 農学部, 教授 (80023472)
Co-Investigator(Kenkyū-buntansha)	渋谷直人農林水産省生物資源研究所, 細胞育種部, 研究員久能均三重大学, 生物資源学部, 教授 (20024573) 吉川正明北海道大学, 理学部, 教授 (00046509) 白石友紀岡山大学, 農学部, 教授 (10033268) 鳥山尚志生物分子応答研究センター, 教授 (40013338)
Keywords	植物細胞応答 / エリシターレセプター / シグナル伝達系 / Ca^<2+>動態 / 活動電位 / リン脂質代謝系 / 活性酸素生成 / 全身的細胞応答
Research Abstract	微生物の感染や生物的エリシターに対する植物の応答現象を軸に、異物認識、シグナル伝達、遺伝子発現並びに新規代謝変動を細胞個別単位の応答とそのシステミックな組織細胞応答の視点で解析し、次の成果を得た。 1.イネおよびダイズ細胞におけるエリシターシグナルの認識・伝達機構を解析し、ダイズ膜のレセプターの純化、部分的アミノ酸配列の決定、レセプターcDNAをクロニングし、イネ原形質膜のレセプター候補タンパク質を同定した。2.タバコ培養細胞のエリシター応答において、PIレスボンスが起こりIP_2生産の上昇ならびに細胞質Ca^<2+>の上昇確認し、ジャガイモ組織や培養細胞で細胞外Ca^<2+>の急速な変動があり、Ca^<2+>チャンネルを通して、細胞にCa^<2+>の上昇を示した。3.エリシター応答と関連して動くポリホスホイノシチド代謝系の酵素精製を行い、エンドウ原形質膜よりPtdInsキナーゼとATPaseが同一分画に得た。4.エリシター応答におけるジャガイモ膜画のホスホリパーゼA^2およびcの活性化様相と酵素的性質を解析し、その精製を進めた。5.車軸藻において活動電位の発生機構を解析し、後過分極の発生は拡散チャンネルのコンダクタンスの低下によって発生することを明らかにした。ジャガイモ細胞のアクチン凝集素の分離・精製し、その候補タンパク質のN-末端アミノ酸分析をした。6.エリシター刺激による活性酸素生成系の活性化には、Ca^<2+>が密接に関与するシグナル伝達系が関与することを示し、原形質膜画分と細胞質可溶性タンパク質画分およびシグナル伝達系因子を基盤に無細胞系における活性化系確立した。活性酸素生成系にスーパーオキシドからヒドロキシラジカルの生成経路を示した。7.エリシター刺激による単細胞レベルの活性酸素生成は、全身的誘導シグナルとなり、全身的活性化を起こすことを示した。

Research Products
(23 results)

All Other

All Publications (23 results)

[Publications] N.Doke: "In vitro activation of NADPH-dependnent O_2-generating system in a plasmamembrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin" Physiol.Mol.Plant Pathol.in press. (1994)
[Publications] Y.Miura: "An autophotographic determination of the active oxygen generation in potato tuber discs during hypersensitive response to fungal infection or elicitor" Pl.Sci.105. 45-52 (1994)
[Publications] H.Yoshioka: "Rapid accumulation of phynylalanine ammonia-lyase mRNA and 3-hydroxy-3-methylglutaryl CoA reductase mRNA by protein systhesis inhibitors" Plant Cell Physiol.35. 1257-1260 (1994)
[Publications] Y.Kamada: "Stimulation by fungal elicitor of inositol phospholipid tumover in tobacco suspension culture cells" Plant Cell Physiol.35. 397-404 (1994)
[Publications] Y.Kamada: "Protein kinase inhibitors inhibit stimulation of inositol phospholipid turnover and induction of phenylalanine ammmonia-lyase in fangfal elicitor-treated tobacco suspension culture cells." Plant Cell Physiol.35. 405-409 (1994)
[Publications] T.Shiraishi: "Suppressor produced by a pea pathogen,Mycosphaerella pinodes." Grain Legumes. 4. 22-23 (1994)
[Publications] T.Yamada: "Functional analysis pf the promoters of phynylananine ammmonia-lyase genes in pea" Plant cell Physiol.35. 917-916 (1994)
[Publications] T.Shiraishi: "Suppressors:determinants of specificity produced by palnt pathogens" Plant cell Physiol. 35. 1107-1119 (1994)
[Publications] H.Kato: "Characterization of nuclea factors in elicitor-mediated activation of the promoter of phenylalanine ammonia-lyase gene 1 in pea(Pisum sativum)." Plant Physiol.107(in press). (1994)
[Publications] K.Nasu: "Suppression of the activation of chitinase and b-1,3-glucanase in pea epicotyls by endogeneous suppressor from Pisum sativum" Ann.Phytopath.soc.Japan. 61(in press). (1994)
[Publications] T.Hiramoto: "The relationship between systemic resistance induced by puruning and accumulation of antifungal substances in barley seedlings" J.Phytopath.143(in press). (1994)
[Publications] T.Hiramoto: "Signal molecules inducing systemic resistance and susceptibility n barley seedings" J.Phytopath.143(in press). (1994)
[Publications] E.Yokota: "Isolataion and characerization of plant myosin from pollen tubes of lily." Protoplasma. 177. 153-162 (1994)
[Publications] T.Mimura: "Characterization of ther Ca2_<+->dependent Cl-eflux in perfused Chara cells." Plant Cell Physiol.35. 793-800 (1994)
[Publications] T.Wayne: "The relation between carbon and water transport in single cells of Chara corallina" Protoplasma. 180. 118-135 (1994)
[Publications] T.Shimmen: "Physiological and biochemical aspects of cytoplasmic streaming" Internat.Rev.cytol.155. 97-139 (1994)
[Publications] T.Shimmen: "Characean cells as atool for studying electrophysiological characteristic streaming" Cell Struct.Fungt.19. 263-278 (1994)
[Publications] T.Shimmen: "Ujique after-hyperpolarization accompaniying action potential in Chara globuralis" J.Plant Res.107. 371-375 (1994)
[Publications] I.Kobayasi: "Induced accessibility and enhanced inaccessibility at the cellular leverl in barley coleoptiles XIV.Evidence for elicitor(s)and suppressor(s)of host inaccessibility from Erisiphe graminis" Physiol.Mol.Plant Pathol.(in press.).
[Publications] 渋谷直人: "イネの生体防御反応を誘導する糖質" 日本農薬学会誌. 69. 67-71 (1994)
[Publications] 白石友紀: "病原菌シグナルと植物の防御応答" 日本農芸化学会誌. 69(in press). (1994)
[Publications] 道家紀志: "植物における感染防御応答と活性酸素" 日本農芸化学会誌. 69(in press). (1994)
[Publications] N.Doke: "Involvement of superoxide in signal transduction:responses to attack by pathogens,physiolosical and chemical shocks,and UV light irradiation in:C.H.Foyer and P.M.Mullineaux,eds,Causes of Photooxidative Stress and Amilioration of Defecnce Systems in Plants" CRC Press, 395 (1994)

1994 Fiscal Year Annual Research Report

植物における細胞間認識と情報伝達

Principal Investigator

道家 紀志 名古屋大学, 農学部, 教授 (80023472)

Research Products

[Publications] N.Doke: "In vitro activation of NADPH-dependnent O_2-generating system in a plasmamembrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin" Physiol.Mol.Plant Pathol.in press. (1994)

[Publications] Y.Miura: "An autophotographic determination of the active oxygen generation in potato tuber discs during hypersensitive response to fungal infection or elicitor" Pl.Sci.105. 45-52 (1994)

[Publications] H.Yoshioka: "Rapid accumulation of phynylalanine ammonia-lyase mRNA and 3-hydroxy-3-methylglutaryl CoA reductase mRNA by protein systhesis inhibitors" Plant Cell Physiol.35. 1257-1260 (1994)

[Publications] Y.Kamada: "Stimulation by fungal elicitor of inositol phospholipid tumover in tobacco suspension culture cells" Plant Cell Physiol.35. 397-404 (1994)

[Publications] Y.Kamada: "Protein kinase inhibitors inhibit stimulation of inositol phospholipid turnover and induction of phenylalanine ammmonia-lyase in fangfal elicitor-treated tobacco suspension culture cells." Plant Cell Physiol.35. 405-409 (1994)

[Publications] T.Shiraishi: "Suppressor produced by a pea pathogen,Mycosphaerella pinodes." Grain Legumes. 4. 22-23 (1994)

[Publications] T.Yamada: "Functional analysis pf the promoters of phynylananine ammmonia-lyase genes in pea" Plant cell Physiol.35. 917-916 (1994)

[Publications] T.Shiraishi: "Suppressors:determinants of specificity produced by palnt pathogens" Plant cell Physiol. 35. 1107-1119 (1994)

[Publications] H.Kato: "Characterization of nuclea factors in elicitor-mediated activation of the promoter of phenylalanine ammonia-lyase gene 1 in pea(Pisum sativum)." Plant Physiol.107(in press). (1994)

[Publications] K.Nasu: "Suppression of the activation of chitinase and b-1,3-glucanase in pea epicotyls by endogeneous suppressor from Pisum sativum" Ann.Phytopath.soc.Japan. 61(in press). (1994)

[Publications] T.Hiramoto: "The relationship between systemic resistance induced by puruning and accumulation of antifungal substances in barley seedlings" J.Phytopath.143(in press). (1994)

[Publications] T.Hiramoto: "Signal molecules inducing systemic resistance and susceptibility n barley seedings" J.Phytopath.143(in press). (1994)

[Publications] E.Yokota: "Isolataion and characerization of plant myosin from pollen tubes of lily." Protoplasma. 177. 153-162 (1994)

[Publications] T.Mimura: "Characterization of ther Ca2_<+->dependent Cl-eflux in perfused Chara cells." Plant Cell Physiol.35. 793-800 (1994)

[Publications] T.Wayne: "The relation between carbon and water transport in single cells of Chara corallina" Protoplasma. 180. 118-135 (1994)

[Publications] T.Shimmen: "Physiological and biochemical aspects of cytoplasmic streaming" Internat.Rev.cytol.155. 97-139 (1994)

[Publications] T.Shimmen: "Characean cells as atool for studying electrophysiological characteristic streaming" Cell Struct.Fungt.19. 263-278 (1994)

[Publications] T.Shimmen: "Ujique after-hyperpolarization accompaniying action potential in Chara globuralis" J.Plant Res.107. 371-375 (1994)

[Publications] I.Kobayasi: "Induced accessibility and enhanced inaccessibility at the cellular leverl in barley coleoptiles XIV.Evidence for elicitor(s)and suppressor(s)of host inaccessibility from Erisiphe graminis" Physiol.Mol.Plant Pathol.(in press.).

[Publications] 渋谷直人: "イネの生体防御反応を誘導する糖質" 日本農薬学会誌. 69. 67-71 (1994)

[Publications] 白石友紀: "病原菌シグナルと植物の防御応答" 日本農芸化学会誌. 69(in press). (1994)

[Publications] 道家紀志: "植物における感染防御応答と活性酸素" 日本農芸化学会誌. 69(in press). (1994)

道家紀志名古屋大学, 農学部, 教授 (80023472)