| Project/Area Number |
06304023
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
DOKE Noriyuki Nagoya Univ., School of Agric.Sci., Professor, 農学部, 教授 (80023472)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKITA Kazuhito Nagya Univ., School of Agric.Sci., Assistant, 農学部, 助手 (90186065)
SHINMEN Teruo Himeji Inst.of Tech., Fuculty of Sci., 理学部, 教授 (80114510)
SHIRAISHI Tomonori Okayama Univ., Collage of Agric., Profesor, 農学部, 教授 (10033268)
KUNOH Hitoshi Mie Univ., Faculty of Bioresources, Professor, 生物資源学部, 教授 (20024573)
TORIYAMA Syoushi Nagiya Univ., Bioscience Center, Professor, 生物分子応答研究センター, 教授 (40013338)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Recognition of elicitor / Oxidative burst / Ca^<2+> dependent protein kinase / Active electronic potential / Cytoskeleton / Systemic acquired resistance / phospholipase / Sub-systemic oxidative burst |
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| Research Abstract |
In this research project, recognition and signal transduction in a cell as well as tissue-cells were investigated using various systems of plant-microbe, biotic elicitor or physical stimuli, and the following major results were obtained.
1) In relation to Ca ^<2+>signalling, effect of Mg ^<2+>on the Ca ^<2+>/H+ antiporter or Ca^<2+>, purification and characterization of Ca^<2+>dependent protein kinase and involvement of Ca ^<2+>dependent protein kinase in inositol phospholipid turnover and PAL induction in elicitor-treated suspension cultured cells was demonstrated. 2) N-acetylchitoorigosaccharide elicited an oxidative burst generating OH radical and phosphorylation of the various proteins in rice suspension cultured cells. 3) Active electric potential was generated and transduced in characean cells by a physical stimulus and resulted in cause a cessation of protoplasmic streaming by Ca^<2+> dependent phosphorylation of myosin. 4) Fungal elicitors stimulated the oxidative burst, followed by lipid peroxidation with an activation of phospholipase A^2. This enzyme activity in potato tissues was dependent on CaM and protein kinase and appeared to be patatin. 5) Cytoskeleton was involved as some signal transduction in rejection-response of plant cells against invading pathogens. 6) In induction of systemic acquired resistance in barley seedlings to pathogens, infection or wounding signals was found to cause first a electric signal and then production of some molecules for secondary signal. 7) The oxidative burst in potato tissues treated with fungal elicitor was found to be dependent on Ca^<2+> influx, CaM and protein kinase and to cause a subsystemic oxidative burst by generated H_2O_2. The subsystemic oxidative burst was also due to Ca^<2+> signalling.
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