1996 Fiscal Year Final Research Report Summary
Analysis on Fc Receptor-mediated Signal Transductions
| Project/Area Number |
06304027
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
Immunology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YODOI Junji Inst.for Virus Research, Dept.of Biological Responses., KYOTO UNIVERSITY, ウイルス研究所, 教授 (80108993)
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| Co-Investigator(Kenkyū-buntansha) |
MASUDA Midori Kansai Medical Univ., Dept.of Clinical Sciences and Laboratory Medicine, Associa, 医学部, 講師 (50173753)
NIKAIDO Toshio Shinshu Univ.School of Medicine, Dept.of Obsterics & Gynecology, Associate Profe, 医学部, 講師 (50180568)
RA Chisei Juntendo Univ.School of Medicine, Dept.of Immunology, Associate Professor, 医学部, 講師 (60230851)
SHIMIZU Akira Kyoto Univ., Center for Molecular Biology and Genetics, Professor, 遺伝子実験施設, 教授 (00162694)
KUMAGAI Shunichi Kobe Univ.School of Medicine, Dept.of Laboratory Medicine, Professor, 医学部, 教授 (00153346)
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| Project Period (FY) |
1994 – 1996
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| Keywords | Fc receptor / signal transduction / IgE / IgG / lymphocyte / immunoblobulin |
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| Research Abstract |
1.Fc epsilon RII/CD23 : We analyzed novel CD23 molecules without the transmembrane region, type a' and b', and determined its intracellular distributions. (Yodoi) Elevated serum levels of soluble CD23 and surface CD23 expressions on monocytes were observed in autoimmune diseases, including SLE and RA,and the corelations with conditions of the patients were suggested. (Kumagai)
2.Fc epsilon RI : As a new function of soluble Fc epsilon RIalpha-chain, we observed that IgE epsilon-chain trans-mRNA is suppressed specifically by soluble Fc epsilon RI alpha-chain. This result showed that FcR may act as a ligand and transduct signals for a certain cellular function. (Shimizu) We analyzed associations of FcR gamma-chain with various FcRs. The interactions with Fc alphaR or FcgammaR are shown to be important for biological cell functions. Also we revealed that nasal mast cells in perennial allergic rhinitics exhibit increased expression of Fc epsilon RI and can induce IgE syntesis. (Ra)
3.FcgammaR : Our studies showed that FcgammaR affects the expressions of p34cdc2, cyclin A,as well as other cell cycle regulatory gene promoters. (Nikaidou) Associations of FcgammaR with Tissue Factor or Lyn tyrosine kinase were observed. Novel FcgammaR-binding molecule (35-25PD) was found. (Masuda)
4.Anti-apoptotic factor (2ME-replacing factor) : Novel redox regulatory factor, which blocks apoptosis and proliferates CTLL cell, was found. Since EBV-positive B cell line produces this small molecular weighted factor, it is suggested to be involved in cell activation and expression of Fc epsilon RII/CD23 on cell transformation caused by EBV or HTLV-I infections. (Yodoi)
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