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1995 Fiscal Year Final Research Report Summary

Decision of blood group genes and apprication of DNA analysis in Forensic Medicine

Research Project

Project/Area Number 06404028
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionReseach Institute of Evolutionary Biology (1995)
Jichi Medical University (1994)

Principal Investigator

IKEMOTO Shigenori  Jichi Medical School Professor, 主任研究員 (90048942)

Co-Investigator(Kenkyū-buntansha) KAJII Eiji  Jichi Medical School Assistant Professor, 医学部, 教授 (40204391)
TSUCHIDA Shuichi  Jichi Medical School Ph.D.Lecture (20217326)
GOTO Kimihiko  Jichi Medical School Lecture (40146155)
IWAMOTO Sadahiko  Jichi Medical School Lecture (10232711)
FUKUI Emiko  Jichi Medical School Assistant (20208341)
Project Period (FY) 1994 – 1995
KeywordsBlood group makers / Gene analysis / Red cell culture / Red cell differentation / Rh gene / RFLP / EsD gene / TF gene polymophism
Research Abstract

The Rh blood group system is of considerable importance in transfusion and clinical medicine. The Rh antigens are carried by a family of hydrophobic membrane proteins (30-32kDa). Molecular studies have resulted in the cloning of several cDNAs for Rh polypeptides ; these can be divided into two different types showing a high level of sequence homology. Both the Rh polypeptides deduced from these two cDNAs consist of 416 amino acid residues predicted to span the membrane bilayr twelve times. Recently, it has been reported that the RH locus is composed of two genes (RHD and RHCE). The RHCE gene appears to encode both the C/c and E/e antigens, probably by alternative splicing. The RHD gene is absent in Caucasian RhD-negative individuals but can be detected in Japanese ones. Several Rh variants have been analyzed and their molecular basis has been revealed. I would like to present the progress in molecular genetics of the Rh blood group system.
The Duffy gene has been shown not to be split by introns, even in its 5'untranslated region and to be expressed not only in erythroid but in postcapillary venule endothelium of almost every organs throughout the body. We reanalyzed the transcriptional start position (TSP) in erythroid and postcapillary venule endothelium by 5'-rapid amplification of cDNA ends procedure and identified a novel exon which encode 7 residues initiated with a methionine, and linked to nucleotide 203 in frame and in agreement with splicing rule. A major TSP in erythroid was identified at 34 base upstream from the first ATG codon, in lung and kidney, at 82 base upstream from the ATG.We cloned 600 base 5'flanking sequence of the novel exon which include one Ap1, two H-APF-1, four Sp-1 and two reversed GATA motifs. The closer GATA sequence, 48 base upstream from the TSP of erythroid, was mutated in black people with Fy (a-b-)phenotype. We performed CAT assay and revealed that the promoter activity in HEL cells was destrupted by the black type mutation.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Ikemoto Shigenori: "Present situation of the analysis of Rh genes" Biochmical Journal. 309. 695-696 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikemoto Shigenori: "RFLP analysis of HLA classII on a case of BMT with an unexpected mixed lymphocyte culture reactive" Japan Jurnal of Electrophoresis. 39. 31-35 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kajii Eiji: "Intricate combinatorial patterns of exon splycing generate multiple Rh-related in human erythroid cells" Human Genetics. 95. 31-35 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池本卯典: "遺伝標識を探して-法医学・人類遺伝学の領域から" 日本法医学雑誌. 49. 419-431 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池本卯典: "血液型" 血液病学(三輪史朗他編). 第2版. 1446-1448 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池本卯典: "同種骨髄委嘱における遺伝標識の挙動と法医学上の問題" 法医学の実際と研究. 38. 1-10 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikemoto Shigenori, Umenishi Fuminori, Kajii Eiji, et al.: "Present situation of the analysis of Rh genes" Biochemical Journal. 309. 695-696 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikemoto Shigenori, Tsuchida Shuichi: "RFLP analysis of HLA class II on a case of BMT with an unexpected mixed lymphocyte culture reactive" Japan Journal of Electrophoresis. 39. 31-35 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kajii Eiji, Ikemoto Shigenori, et al.: "Intricate combinatorial patterns of exon splycing generate multiple Rh-related in human erythroid cells" Human Genetic.95. 657-665 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikemoto Shigenori: "Shearching for genetic makers" Japan Journal Legal medicine. 49. 419-431 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikemoto Shigenori: "Behavior of genetic makers in recipients after BMT" Reseach Practical Forensic Medicine. 38. 1-10 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Omi Toshinori, Ikemoto Shigenori et al.: "Differentical splicing of the glycophorin A mRNA" Electrophoresis. 15. 1091-1094 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto Sadahiko, Ikemoto Shigenori, et al.: "Genetic organization of the glycoprotein Gene" Blood. 85. 622-626 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchida Shuichi, Fukui Emiko, Ikemoto Shigenori: "Molecular analysis of esrerase D polymorphism" Human Genetic. 93. 255-258 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto Sadahiko, Ikemoto Shigenori, Kajii Eiji, et al.: "Identification of a novel exon and spliced form of Duffy mRNA that is predominant transcript in both erythroid and postcapillary venule endohrelium" Blood. 87. 378-385 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikemoto Shigenori, Anstee, JD.: "Molecular biology of red cell blood groups" ISBT'96. (Abstract) : 41. (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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