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1996 Fiscal Year Final Research Report Summary

Regeneration of pigment cells and basic and clinical study for treatment of age-related macular degeneration

Research Project

Project/Area Number 06404061
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTohoku University

Principal Investigator

TAMAI Makoto  School of Medicine Tohoku Univ.Dept.Opthalmol.Prof., 医学部, 教授 (90004720)

Co-Investigator(Kenkyū-buntansha) YUSUF Durlu K  Same as above Faculty, 医学部・付属病院, 助手 (90250787)
YAMADA Takahiko  Same as above Assistant Prof., 医学部・付属病院, 講師 (40191316)
ISHIGURO Seiichi  Same as above Assistant Prof., 医学部, 講師 (20111271)
Project Period (FY) 1994 – 1996
KeywordsRPE / gene transfer / bFGF / RPE transplantation / IPE / IPE transplantation / Cryopreservation / Age-related macular degeneration
Research Abstract

Age-related macular degeneration is one of the most serious diseases in the elderly people. Even if submacular and choroidal neovascular membrane could be surgically excised, severe damage or evacuation of retinal pigment epithelium are innevitable in the operated area. Pigmentary dystrophy is also a devastating hereditary eye disease with severe visual disturbance. Up to now, we have no effective treatments for both of them. We have conducted basic experiments of retinal pigment epithelium (RPE) culture, their transplantation to subretinal space of animals, especially, the royal college of surgeon's (RCS) rat, a model of hereditary retinal degeneration and observed their rescue effects for photoreceptor cell death.
1) With the retinal dystrophic RCS rat, we transplanted RPE from Long Evans rat, human, and bovine and could observe the retardation of the photoreceptor cell death.
2) RPE cells from eye bank eye or fetus eye were easily obtained and cultured. They were transplanted as fresh … More or cultured with primary or multiple passage. We also tried cryopreservation of these cells up to 3 months. Their characteristics were well preserved at least in the viewpoints of several gene expression. In the future, if we could keep these RPE cells in deep-freezed condition, we could use them clinically in appropriate time and number of cells for patients just as "RPE bank".
3) In experimental animals, they did not show any immunological reaction. But transplantation of human RPE cells with collagen sheet into the anterior chamber in rabbit showed difinite reaction like suppression of electroretinogram and macrophage infiltration in the subretinal space, not only in the operated but contralateral non-operated eye. These results suggest we must be cautious in clinical application of heterogenous RPE transplantation. It was an interesting observation that the expression of MHC class II cells were observed in the course of photoreceptor cell degeneration in the RCS rat but supressed if they were rescued by the transplantation of human cultured RPE in these animals.
4) If we cpnsider clinical application of this technique, it is natural the autograft is much better than the xeno-or allograft. We tried to use the iris pigment epithelium (IPE) for transplantation because they were neural origin, pigmented cells and easily and enough amount of them were obtained by peripheral iridectomy. IPE of rat were cultured the vector (pCNX2) with cDNA of rat bFGF were transfected and transplanted into the subretinal space of RCS rat. These transfected cells expressed strong mRNA of bFGF.The photoreceptors were well preserved and no immunological reaction. If we could use IPE,either wild or modified with molecular technique, they were applicable for patients, much safer and easier. Less

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Iamai M.et al: "bFGF tiansfected iris PE may rescue photoreoptor cell degeveialin" Retinal degeneiation. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Durlu YK,Tamai M: "In vitro expression of epidermal growth factor receptor by" Degenerative disease of the retina. 69-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Durlu YK,Tamai M: "Transplantation of retiral pigment epithelium using viable" Cell Transplantation. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe T.et al: "The property of retinal pigment epithelial colls" Exp.Eye Res.63. 201-210 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 玉井 信: "網膜色素上皮移植" 日眼会誌. 100. 982-1006 (1996)

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      「研究成果報告書概要(和文)」より
  • [Publications] 山田孝彦・他: "網膜下血腫によって漏斗状網膜剥離にいたった加齢黄斑変性" 臨床眼科. 50. 1249-1253 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamai M,Yamada K,Takeda Y,Tomita H,Abe T,Kojima S and Ishiguro S-I.: "bFGF transfected iris PE may rescue photoreceptor cell degeneration in RCS rats." Anderson RE,Hollyfield JG and La Vail MM eds.Retinal.Degeneration. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Durlu YK and Tamai M.: "Transplantation of retinal pigment epithelium using viable cryopeserved cells." Cell Transplant.(In press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe T,Durlu YK,Tamai, M.: "The property of retinal pigment epithelial cells in proliferative vitreoretinopathy compared with cultured retinal pigment epithelial cells." Exp.Eye Res.63. 201-210 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiko Akaishi: "RPE transplantation and immunity." Ganka. 37. 1349-1352 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahiro Yamada, Toshio Kanno, Makoto Tamai.: "Magnetic resonance imaging of subretinal lesions in age-related disciform macular degeneration." Jpn J Clini Opthalmol. 49. 1092-1094 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahiko Yamada, Toshio Kanno, Makoto Tamai: "Results of surgery for funnel-shaped hemorrhagic retinal detachment secondary to age-related macular degeneration." Jpn J Clini Opthalmol. 50. 1249-1253 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiyuki Takeda, Katsuhiro Yamaguchi, Katsura Yamada, Makoto Tamai: "Photoreceptor cell rescue in royal college of surgeons rat retina by human subretinal fluid." Folia Opthalmol.Jpn. 47. 418-420 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makoto Tamai: "Retinal pigment epithelial cell transplantation : Perspective." J Jpn Opthalmol Soc. 100. 982-1006 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshio Kanno, Takahiko Yamada, Makoto Tamai: "Use of the TUNEL methods to detect changes in rabbit neural retina in an experimental model of retinal pigment epithelial damage." Folia.Opthalmol.Jpn.47. 205-208 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makoto Tamai: "Experimental approach to retinal pigment epithelial cell transplantation." Shinkei Kenkyuuno Shinpo. 40. 813-821 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Madoka Yoshida, Toshio Kanno, Takehiko Sakuma, Takahiko Yamada, Makoto Tamai: "Scanning laser opthalmoscopic findings after surgical removal of submacular lesions of age-related macular degeneration." Folia.Opthalmol.Jpn.48 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Durlu YK and Tamai M.: In vitro expression of epidermal growth factor receptor by human retinal pigment epithelial cells. Anderson RE,Hollyfield JG and La Vail MM eds., Degenrative Deseases of the Retina. Plenum Press, New York, 69-76 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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