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1995 Fiscal Year Final Research Report Summary

Molecular mechanisms of osteoporosis induced by estrogen deficiency.

Research Project

Project/Area Number 06404067
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

SUDA Tatsuo  Showa University, School of Dentistry, Professor, 歯学部, 教授 (90014034)

Co-Investigator(Kenkyū-buntansha) KATAGIRI Takenobu  Showa University, School of Dentistry, Assistant, 歯学部, 助手 (80245802)
TAKAHASHI Naoyuki  Showa University, School of Dentistry, Associate Professor, 歯学部, 助教授 (90119222)
MIYAURA Chisato  Showa University, School of Dentistry, Lecturer, 歯学部, 講師 (20138382)
SHINKI Toshimasa  Showa University, School of Dentistry, Lecturer, 歯学部, 講師 (90138420)
Project Period (FY) 1994 – 1995
Keywordsestrogen / androgen / bone resorption / interleukin-1 / interleukin-6 / osteoporosis / hemopoiesis / bone marrow
Research Abstract

1.Endogenous bone-resorbing factors in estrogen deficiency
Estrogen deficiency causes a marked bone loss by stimulating osteoclastic bone resorption. In this study, we examined the bone-resorbing activity present in the supernatant fraction of mouse bone marrow collected from ovariectomized (OVX) mice. Adding bone marrow supernatants at 20-80% to organ cultures of mouse long bones dose-dependently stimulated bone resorption. Anti-IL-lalpha antibody completely neutralized the bone-resorbing activity present in bone marrow supernatants from OVX mice. Antibodies against IL-lbeta, IL-6 and IL-6 receptors also neutralized it, but partially. The concurrent addition of IL-1, IL-6 and soluble IL-6 receptor, which equaled the endogenous concentrations on bone marrow supernatants from OVX mice, co-operatively induced bone resorption. These results suggest that the enhanced bone resorption that occurs during estrogen deficiency is due to multi-factors rather than to a single factor.
Increased hemopoiesis in sex steroid deficiency.
Estrogen deficiency caused by OVX selectively stimulates B lymphopoiesis, resulting in a marked accumulation of pre-B cells in mouse bone marrow. We compared the effects of estrogen and androgen on B lymphopoiesis in bone marrow and bone metabolism using orchidectomised (ORX) mice. Some of the ORX mice were treated with estrogen or androgen. At3 weeks after operation, in ORX mice, the number of bone marrow cells were markedly increased, and most of the increased cells were B220-positive pre-B lymphocytes. The characteristics of the accumulated cells and the time course of changes of the stimulated B lymphopoiesis after operation were quite similar to those in OVX mice. These changes of hemopoiesis and bone metabolism induced by ORX were completely restored by the treatment of estrogen. These results suggest that estrogen regulates B lymphopoiesis and bone metabolism not only in female but also in male mice.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Onoe Y.,et al.: "IL-13 and IL-4 inhibit bone resorption by suppressing cyclooxy-genase-2-dependent prostaglandin synthesis in osteoblasts." J.Immunol.156. 758-764 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyaura C.,et al.: "Endogenous bone-resorbing factors in estrogen deficiency : Cooperative effects of IL-1 and IL-6." J.Bone Miner.Res.10. 1365-1373 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suda T.,et al.: "Modulation of osteoclast differentiation by local factors." Bone. 17. 87S-91S (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyaura C.,et al.: "Mechanism of bone resorption induced by estrogen deficiency." J.Bone Miner.Met.12. S17-S21 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuzawa T.,et al.: "Estrogen deficiency stimulates B lymphopoiesis in mouse bone marrow." J.Clin.Invest.94. 1090-1097 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura T.,et al.: "Soluble interleukin-6 receptor triggers osteoclast formation by interleukin-6." Proc.Natl.Acad.Sci.USA. 90. 11924-11928 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Onoe, Y.et al.: "IL-13 and IL-4 inhibit bone resorption by suppressing cyclooxygenase-2-dependent prostaglandin synthesis in osteoblasts." J.Immunol.156. 758-764 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyaura, C.et al.: "Endogenous bone-resorbing factors in estrogen deficiency : Cooperative effects of IL-1 and IL-6." J.Bone Miner.Res.10. 1365-1373 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suda, T.et al.: "Modulation of osteoclast differentiation by local factors." Bone. 17. 87S-91S (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyaura, C.: "Mechanism of bone resorption induced by estrogen deficiency" J.Bone Miner.Met.12. S17-S21 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masuzawa, T.et al.: "Estrogen deficiency stimulates B lymphopoiesis in mouse bone marrow." J.Clin.Invent.94. 1090-1097 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura, T.et al.: "Soluble interleukin-6 receptor triggers osteoclast formation by interleukin-6." Proc.Natl.Acad.Sci.USA. 90. 11924-11928 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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