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1995 Fiscal Year Final Research Report Summary

The mechanism of neurotransmitter release and its control using electric organ synapse of electric ray

Research Project

Project/Area Number 06404076
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

KIRINO Yutaka  Univ.Tokyo ; Fac.Pharmaceutical Sci. ; Professor, 薬学部, 教授 (10012668)

Co-Investigator(Kenkyū-buntansha) TOKUMARU Hiroshi  Univ.Tokyo ; Fac.Pharmaceutical Sci. ; Assistant Professor, 薬学部, 助手 (70262160)
KAWAHARA Shigenori  Univ.Tokyo ; Fac.Pharmaceutical Sci. ; Assistant Professor, 薬学部, 助手 (10204752)
Project Period (FY) 1994 – 1995
Keywordselectric organ / synaptosome / calcium channel / acetylcholine / cDNA library / intra-synaptosomal calcium
Research Abstract

1. cDNA cloning of voltage-dependent Ca channel of electromotor neuron from electric ray.
RT-PCR was carried out with electric lobe of Japanese electric ray, Narke Japonica. Partial sequence of N- and L-type voltage dependent Ca channel were obtained.
2. Experimental setup to investigate the electrophysiological characteristics of Ca channel of electric organ nerve terminal.
In order to do the electrophysiological characterization of Ca channel of electric organ synaptosomes, lipid bilayer system was introduced.
Preliminary experiments revealed that a voltage dependent anion channel was detected in ciliary membrane vesicles from Teterahymena.
3. Asymmetrical distribution of phospholipids in membrane of synaptosome and synaptic vesicle from electric ray.
The asymmetrical distribution of phospholipids between the outer and inner leaflets of synaptosome and synaptic vesicle was investigated. In both membranes, phosphatidylserine (PS) was dominant in the leaflet which faces cytoplasm.
4. The effects of adenosine on the acetylcholine release from electric organ synaptosomes.
The effects of adenosine on the acetylcholine (ACh) release from synaptosomes was investigated. Adenosine inhibited the ACh release through adenosine Al receptor and facilitated the release through A2 receptor. The inhibitory effect via Al receptor was caused by the inhibition of N-type voltage dependent Ca channel, and the facilitatory effects via A2 receptor was caused by the activation of P-type Ca channel.

  • Research Products

    (60 results)

All Other

All Publications (60 results)

  • [Publications] Kohki Shinozaki: "Ion Channel Activity of a Synthetic Peptide with a Primary Structure Corresponding to the Presumed Pore-Forming Region of the Voltage" Biochem.Biophys.Res.Commun.198. 445-450 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohki Shinozaki: "Synthesis and Property of H5 Region-Related Peptide of K+ Channel and Its Ion Channel-Forming Ability" Peptide Chemistry 1993. 365-368 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihide Iwata: "Design and Synthesis of Amphipathic 310Helical Peptides and Their Interactions with Phospholipid Bilayers and Ion Channel Formation" J.Biol.Chem.269(7). 4928-4933 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazunori Anzai: "Asymmetrical Lipid Charge Changes the Substate Conductance of the Potassium Channel from Sarcoplasmic Reticulum" Biochem.Biophys.Res.Commun.199. 1081-1087 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sannamu Lee: "Two Mode Ion Channels Induced by Interaction of Acidic Amphipathic-Helicals Peptides with Lipid Bilayers" Biochim.Biophys.Acta. 1191(1). 181-189 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaji Inoue: "A Comparative ESR Study of Some Paramagnetic Materials as a Probe for Non-ivasive Measurement of Dissolved Oxygen Concentration" Chem.Pharm.Bull.42(11). 2346-2348 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazunori Anzai: "Rapid measurement of Internal Volumes of Membrane Vesicles with ESR-Stopped Flow Technique" Magn.Reson.Medicine. 6. 190-191 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihide Kusumoto: "Simultaneous Measurement of Changes in Intracellular Calcium Ions and Degranulation in Single Mast Cells from Rat Peritoneum" Bioimages. 3(1). 1-5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "生体内酸素濃度測定法の開発" 学術月報. 48(3). 81 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Tokumaru: "A Calcium Channel from the Presynaptic Nerve Terminal of the Narke japonica Electric Organ Contains a Non-N-type α2δ Subunit" J.Neurochem.65(2). 831-836 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazunori Anzai: "Phosphatidylserine-specific Transbilayer Lipid Translocation in Synaptosomal Plasma Membranes from Narke japonica" J.Biochem.117. 1232-1237 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiji Sakamoto: "Enhanced Membrane-Perturbing Activities of Bundled Amphiphilic-Helix Polypeptides on Interaction with Phospholipid Bila" Bull.Chem.Soc.Japan. 68(10). 2931-2939 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "座談会:基礎薬学分野における物理化学のあり方" ファルマシア. 31. 1117-1122 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "膜電位イメージング法によるナメクジ中枢神経系における嗅覚情報処理機構の研究" ファルマシア. 32(3). 37-40 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chie Fujiwara-Hirashima: "A Voltage-dependent Anion Channel from Tetrahymena Ciliary Membrane Incorporated into Planar Lipid Bilayers" Biochim.Biophys.Acta.1280(2). 207-216 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川原茂敬: "膜電位感受性蛍光色素を用いたナメクジ中枢神経系の解析" バイオイメージング. 5(1). 1-6 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shoichi Toda: "Optical Recording of Neural Activity in the Olfactory Center of a Terrestrial Slug,Limax marginatus" Bioimages. 4(1). 33 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoshi Watanabe: "Development of Low Magnesium-induced Spontaneous Synchronized Bursting and GABAergic Modulation in Cultured Rat Neocortical Neurons" Neurosci.Lett.210(1). 41-44 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuya Kimura: "Spatial and Temporal Analysis of Odor Response in the Olfactory Center of the Terrestrial Slug,Limax marginatus" Proc.Internatl.Symp.Bioelect.Mol.Elect.Dev.140-141 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yutaka Hirakura: "Selective Interaction of Synthetic Antimicrobial Peptides Derived from Sapecin B with Lipid Bilayers" J.Biochem.120(6). 1130-1140 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yutaka Kirino: "An Optical Study of Odor and Taste Information Processing in the Ganglia of the Slug,Limax marginatus" Progr.Biophys.Mol.Biol.65. 185 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yutaka Hirakura: "Selective Interaction of Synthetic Antimicrobial Peptides Derived from Sapecin B with Lipid Bilayers" Progr.Biophys.Mol.Biol.65. 117 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atsuhisa Tomita: "Removal after Addition of NO-generating Agents and 8-Bromo-cyclic GMP Cause Morphological Change of Cultured Cerebellar Astrocytes" Brain Res.744. 344-346 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuyoshi Inoue: "Neural Oscillation in the Procerebrum of the Terrestrial Slug as Visualized by Calcium Imaging" Bioimages. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigenori Kawahara: "Comparative Study on Oscillation in the Procerebrum of the Terrestria Slugs Incilaria bilineata and Limax marginatus" J.Exp.Biol.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jean de Barry: "Time Resolved Imaging of Protein Kinase C Activation during Sea Urchin Egg Fertilization" Exp.Cell Res.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiharu Suzuki: "Phosphorylation of Alzheimer's -Amyloid Precursor-like Proteins" Biochemistry. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Tokumaru: "Involovement of Calciseptine-sensitive Calcium Channels in Acetylcholine Release from Electric Organ Synaptosomes" Biol.Pharm.Bull.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "続医薬品の開発、第17巻.薬物の構造解析と医薬品開発" 廣川書店, 306 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "シナプス伝達のダイナミックス" 培風館, 146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "情報と薬学いま(CD-ROM)" 情報薬学研究会, (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "第13改正日本薬局方解説書" 廣川書店, (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "情報生物学シリーズ3.カルシウムの物理化学的性質" 培風館(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桐野 豊: "基礎薬学 物理化学" 廣川書店(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yutaka Kirino: "Role of Adenosine in the Nervous System" Elsevier (印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohki Shinozaki: "Ion Channel Activity of a Synthetic Peptide with a Primary Structure Corresponding to the Presumed Pore-Forming Region of the Voltage-Dependent Potassium Channel" Biochem.Biophys.Res.Commun.198. 445-450 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohki Shinozaki: "Synthesis and Property of H5 Region-Related Peptide of K+ Channel and Its Ion Channel-Forming Ability" Peptide Chemistry 1993 (Y.Okada, Ed.) Protein Research Foundation. 365-368 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshihide Iwata: "Design and Synthesis of Amphipathic 310 Helical Peptides and Their Interactions with Phospholipid Bilayrs and Ion Channel Formation" J.Biol.Chem.269 (7). 4928-4933 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazunori Anzai: "Asymmetrical Lipid Charge Changes the Substate Conductance of the Potassium Channel from Sarcoplasmic Reticulum" Biochem.Biophys.Res.Commun. 199. 1081-1087 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sannamu Lee: "Two Mode Ion Channels Induced by Interaction of Acidic Amphipathic-Helical Peptides with Lipid Bilayers" Biochem.Biophys.Acta. 1191 (1). 181-189 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaji Inoue: "A Comparative ESR Study of Some Paramagnetic Materials as a Probe for Non-ivasive Measurement of Dissolved Oxygen Concentration in Biological systems" Chem.Pharm.Bull.42 (11). 2346-2348 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazunori Anzai: "Rapid measurement of Internal Volumes of Membrane Vesicles with ESR-Stopped Flow Technique" Magn.Reson.Medicine. 6. 190-191 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akihide Kusumoto: "Simultaneous Measurement of Changes in Intracellular Calcium Ions and Degranulation in Single Intact Mast Cells from Rat Peritoneum" Bioimages. 3 (1). 1-5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Tokumaru: "A Calcium Channel from the Presynaptic Nerve Terminal of the Narke japonica Electric Organ Contains a Non-N-type alpha_2delta Subunit" J.Neurochem. 65 (2). 831-836 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazunori Anzai: "Phosphatidylserine-specific Transbilayer Lipid Translocation in Synaptosomal Plasma Membranes from Narke japonica" J.Biochem.117. 1232-1237 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiji Sakamoto: "Enhanced Membrane-Perturbing Activities of Bundled Amphiphilic -Helix Polypeptides on Interaction with Phospholipid Bilayer" Bull.Chem.Soc.Japan. 68 (10). 2931-2939 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chie Fujiwara-Hirashima: "A Voltage-dependent Anion Channel from Tetrahymena Ciliary membrane Incorporated into Planar Lipid Bilayrs" Biochim.Biophys.Acta.1280 (2). 207-216 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shoichi Toda: "Optical Recording of Neural Activity in the Olfactory Center of a Terrestrial Slug, Limax marginatus" Bioimages. 4 (1). 33 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoshi Watanabe: "Development of Low Magnesium-induced Spontaneous Synchronized Bursting and GABAergic Modulation in Cultured Rat Neocortical Neuron" Neurosci.Lett.210. 41-44 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuya Kimura: "Spatial and Temporal Analysis of Odor Response in the Olfactory Center of the Terrestrial Slug, Limax marginatus" Proc.5th Int.Symp.Bioelect.Mol.Electr.Dev.6th Int.Conf.Mol.Electr.Biocomp.Research & Development Association for Future Electron Dvices. 140-141 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yutaka Hirakura: "Selective Interaction of Synthetic Antimicrobial Peptides Derived from Sapecin B with Lipid Bilayrs" J.Biochem.120 (6). 1130-1140 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yutaka Kirino: "An Optical Study of Odor and Taste Information Processing in the Cerebral Ganglia of the Slug, Limax marginatus" Progr.Biophys.Mol.Biol.65. 185 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yutaka Hirakura: "Selective Interaction of Synthetic Antimicrobial Peptides Derived from Sapecin B with Lipid Bilayrs" Progr.Biophys.Mol.Biol.65. 117 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuyoshi Inoue: "Neural Oscillation in the Procerebrum of the Terrestrial Slug as Visualized by Calcium Imaging" Bioimages. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsuhisa Tomita: "Removal after Addition of NO-generating Agents and 8-Bromo-cyclic GMP Cause Morphological Change of Cultured Cerebellar Astrocytes : a New Mode of NO Action" Brain Res.744. 344-346 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigenori Kawahara: "Comparative Study on Oscillation in the Procerebrum of the Terrestrial Slugs Incilaria bilineata and Limax marginatus" J.Exp.Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jean de Barry: "Time Resolved Imaging of Protein Kinase C Activation during Sea Urchin Egg Fertilization" Exp.cell Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiharu Suzuki: "Phosphorylation of Alzheimer's-Amyloid Precursor-like Proteins" Biochemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Tokumaru: "Involovement of Calciseptine-sensitive Calcium Channels in Acetylcholine Release from Electric Organ Synaptosomes" Biol.Pharm.Bull.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yutaka Kirino: "Activation of Adenosine A1 and A2 Receptors Differentially Modulates Acetylcholine Release from Electric Organ Synaptosomes of Japanese Electric Ray Narke japonica" "Role of Adenosine in the Nervous System" Elsevier. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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