1995 Fiscal Year Annual Research Report

パルスENDORによる光合成水分解計の局所構造と機能の研究

Research Project

Project/Area Number	06452073
Research Institution	Faculty of Science, Kwansei Gakuin University
Principal Investigator	河盛阿佐子関西学院大学, 理学部, 教授 (40079661)
Keywords	パルスENDOR / パルスELDOR / 光化学系II / チロシン2 / プラストキノン / 水分解系 / ESEEM / 光合成
Research Abstract	光合成光化学計II粒子の非ヘム鉄抜き,シアン(CN)処理,および鉄を亜鉛置換した試料について陽子パルスENDORを観測し,バッファーを重水置換した試料の結果と比較してQ_A周辺の交換可能な水素を確定した.トリス処理した光化学系II粒子でトラップしたチロシンZ^+のEPR線形が低いpHで変化することよりパルスENDORで詳細に調べ,これが陽イオンラジカルであることを確認した.電子移動で水素結合の関与を微視的に調べるのにパルスENDORの有効性を示した上記2つの結果は第10回国際光合成会議で報告した. 本年新しい二重共鳴の方法として異なる共鳴周波数を持つラジカルまたは常磁性金属イオンに同時にパルスEPRを行って,これらの電子伝達担体間の距離を双極子相互作用の大きさから見積もる方法を完成させ,Caを水分解系から除去した試料に使用して,S_3状態のラジカルとチロシンD,またS_1状態でトラップぢたチロシンZ^+とD,水分解系マンガンとDの距離をそれぞれ30,29,27Aと決定出来た.過去の間接的な測定に比べ,精度は0.5A以内と上昇した.

Research Products

(8 results)

All Other

All Publications (8 results)

[Publications] Andrei. V. Astashkin: "An electron spin echo envelope modulation study of the primary acceptor quinone in Zn-substitued Photosystem II." J. Chem. Phys.102. 5583-5588 (1995)
[Publications] Yoshio. Kodera,: "EPR study of trapped tyrosine Z^+ in Ca-depleted Photosystem II" Biochim. Biophys. Acta.1232. 43-51 (1995)
[Publications] Sergei. A. Dzuba: "Mogecular Motion of the Cholestane Spin Label in a Multibilayer in the Gel Phase Studied Using Echo-Detected EPR." J.Mabnetic Resonance. A115. 80-86 (1995)
[Publications] Masahiro Mikuriya: "Synthsis of a Tetranuclear Manganese Complex with a Cubane Core at the Mn(II)Mn(III)Mn(II)Mn(III)Oxidation level." Chemistry Letters. 1095-1096 (1995)
[Publications] Asako Kawamori: "Multiple Resonance in Pulsed EPR with Application to Photosynthesis" Kwansei Gakuin Annual Studies. 44. 221-240 (1996)
[Publications] Sergei A. Dzuba: "Selective Hole Burning in EPR: Spectral Diffusion and Dipolar Broadening" Concepts Magne. Reson.8. 49-61 (1996)
[Publications] Asasko Kawamori: "Photosynthesis: from Light to Biosphere, Vol I ed. P. Mathis" Kuluwer Academic Publishers, 4/1004 (1995)
[Publications] Hiroyuki Mino: "Photosynthesis: from Light to Biosphere, Vol I ed. P. Mathis" Kuluwer Academic Publishers, 4/1004 (1995)

1995 Fiscal Year Annual Research Report

パルスENDORによる光合成水分解計の局所構造と機能の研究

Principal Investigator

河盛 阿佐子 関西学院大学, 理学部, 教授 (40079661)

Research Products

[Publications] Andrei. V. Astashkin: "An electron spin echo envelope modulation study of the primary acceptor quinone in Zn-substitued Photosystem II." J. Chem. Phys.102. 5583-5588 (1995)

[Publications] Yoshio. Kodera,: "EPR study of trapped tyrosine Z^+ in Ca-depleted Photosystem II" Biochim. Biophys. Acta.1232. 43-51 (1995)

[Publications] Sergei. A. Dzuba: "Mogecular Motion of the Cholestane Spin Label in a Multibilayer in the Gel Phase Studied Using Echo-Detected EPR." J.Mabnetic Resonance. A115. 80-86 (1995)

[Publications] Masahiro Mikuriya: "Synthsis of a Tetranuclear Manganese Complex with a Cubane Core at the Mn(II)Mn(III)Mn(II)Mn(III)Oxidation level." Chemistry Letters. 1095-1096 (1995)

[Publications] Asako Kawamori: "Multiple Resonance in Pulsed EPR with Application to Photosynthesis" Kwansei Gakuin Annual Studies. 44. 221-240 (1996)

[Publications] Sergei A. Dzuba: "Selective Hole Burning in EPR: Spectral Diffusion and Dipolar Broadening" Concepts Magne. Reson.8. 49-61 (1996)

[Publications] Asasko Kawamori: "Photosynthesis: from Light to Biosphere, Vol I ed. P. Mathis" Kuluwer Academic Publishers, 4/1004 (1995)

[Publications] Hiroyuki Mino: "Photosynthesis: from Light to Biosphere, Vol I ed. P. Mathis" Kuluwer Academic Publishers, 4/1004 (1995)

河盛阿佐子関西学院大学, 理学部, 教授 (40079661)