1995 Fiscal Year Final Research Report Summary
DYNAMIC ASPECTS OF SPECIFIC RECOGNITION PROCESSES AT LIPID MEMBRANE INTERFACES
| Project/Area Number |
06453153
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
高分子構造・物性(含繊維)
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| Research Institution | TOYAMA UNIVERSITY |
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Principal Investigator |
KITANO Hiromi TOYAMA UNIVERSITY,FACULTY OF ENGINEERING,DEPARTMENT OF CHEMICAL AND BIOCHEMICAL ENGINEERING,PROFESSOR, 工学部, 教授 (40115829)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Yasushi TOYAMA UNIVERSITY,FACULTY OF ENGINEERING,DEPARTMENT OF CHEMICAL AND BIOCHEMICAL, 工学部, 助手 (60242484)
MATSUGO Seiichi TOYAMA UNIVERSITY,FACULTY OF ENGINEERING,DEPARTMENT OF CHEMICAL AND BIOCHEMICAL, 工学部, 助教授 (30148126)
ITO Kensaku TOYAMA UNIVERSITY,FACULTY OF ENGINEERING,DEPARTMENT OF CHEMICAL AND BIOCHEMICAL, 工学部, 助教授 (10192494)
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| Project Period (FY) |
1994 – 1995
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| Keywords | SURFACE-ENHANCED RAMAN SCATTERING (SERS) / MEMBRANE PROTEINS / GLYCOLIPID / MULTIPLE INTERNAL REFLECTION FLUORESCENCE (MIRF) / MOLECULAR RECOGNITION / LECTIN / HAPTEN / レクチン |
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| Research Abstract |
DYNAMIC ASPECTS CONCERNING SPECIFIC MOLECULAR RECOGNITION PROCESSES AT LIPID MEMBRANE INTERFACES WERE INVESTIGATED BY MAKING USE OF VIBRATION SPECTROSCOPY (SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS) AND FOURIER-TRANSFORM INFRA RED (FTIR) SPECTROSCOPY,FOR EXAMPLE), MULTIPLE INTERNAL REFLECTION FLUORESCENCE METHOD (MIRF), AND MICROSCOPIC METHOD.
(1) CYTOCHROME C BOUND TO SELF-ASSEMBLED MONOLAYERS (SAM) ON SILVER WAS STUDIED BY SERS,AND IT WAS CONCLUDED THAT SILVER MODIFIED WITH A SAM IS A SUITABLE SERS-ACTIVE SUBSTRATE TO ANALYZE THE STRUCTURE OF PROTEINS ON BIOMIMETIC MEMBRANE SURFACES WITHOUT DENATURATION.
(2) NOVEL AMPHIPHILES WHICH CONTAIN GALACTOSE RESIDUES WERE PREPARED BY TELOMERIZATION OF GALACTOSE-CARRYING VINYL MONOMER USING A LIPOPHILIC RADICAL INITIATOR.THE GALACTOSE-CARRYING AMPHIPHILES INCORPORATED IN A LIPOSOME WAS EASILY RECOGNIZED BY A LECTIN FROM Ricinus communis AND GALACTOSE OXIDASE.THE RECOGNITION WAS LARGELY AFFECTED BY THE DEGREE OF POLYMERIZATION AND THE SURFACE DENSITY OF THE AMPHIPHILE.
(3) BINDING PROCESSES OF ANTIBODY TO A HAPTEN-DOPED LIPID MONOLAYERS WERE FOLLOWED BY THE MULTIPLE INTERNAL REFLECTION FLUORESCENCE METHOD.THE INITIAL RATE OF BINDING OF THE ANTIBODY INCREASED AT A LOW SURFACE DENSITY OF HAPTENS IN THE LIPID MONOLAYER,AND LEVELED OFF AT A MODERATE-TO-HIGH HAPTEN DENSITY.THE LONG SPACER BETWEEN THE HAPTEN GROUP AND THE LIPID LAYER PROMOTED THE ACCESS OF ANTIBODIES TO THE HAPTENS.
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