1994 Fiscal Year Annual Research Report

三価鉄還元酵素遺伝子導入(鉄欠乏耐性)植物の創成

Research Project

Project/Area Number	06453161
Research Institution	The University of Tokyo
Principal Investigator	森敏東京大学, 農学部, 教授 (90011915)
Co-Investigator(Kenkyū-buntansha)	藤原徹東京大学, 農学部, 助手 (80242163)
Keywords	Agrobacterium / FRE1 / Fe^<3+>-reductase / ylast / Nicotiana tabacum / pBI121 / Fe-deficiencuy
Research Abstract	酵母の細胞膜に存在する3価鉄還元酵素の核遺伝子FRE1をふくむplasmid, pC6Lから制限酵素でORFにあたる配列を切り出し,2種類のT-DNAのconstruct,PYH6(pBI121由来)とPYH7(pUCΩ E12GUS由来)に組み込み,triparential mating法によりAgrobacteriumへ導入し,Agrobacteriumをタバコ(Nicotiana tabacum L.var SR1)に感染させた.カナマイシン耐性カルスを選抜し,植物体を再生させT2植物種子を採取し,カナマイシン耐性の7個体を得た.FRE1に特異的なprimerでPCRを行い,根からのRNAに対してnorthern hybridizationを行うと,3.4kbの転写物が確認された.これらの植物根の細胞膜が導入遺伝子の恒常的な発現によって,鉄が十分にある条件下でも3価鉄還元酵素活性を有しているかどうかを,根圏近傍のBPDS-Fe^<2+>の発色法を用いて検定した.残念ながら,発色は有意ではなかった.そこで,鉄欠乏条件下での本酵素活性の持続性を水耕法によって検定したところ,2株が鉄欠乏耐性の傾向を示した.鉄欠乏条件下で3価鉄還元酵素活性が発現するためには,NADHなどのコファクターの濃度の増大や,根圏還元力としてのプロトンの放出(ATPase活性の増大)などが共役的に起こらなければならないのかもしれない.これらの観点から,さらに実験を進める必要がある.

Research Products

(8 results)

All Other

All Publications (8 results)

[Publications] Higuchi K.,et al: "Purification and characterization of NA Synthase in the Roots of Gramineae" Plant and Soil. 165. 173-179 (1994)
[Publications] Okumura N.,et al.: "A dioxygenase gene(Ids2)expressed under iron deficiency conditions in the roots of Hordeum vulgare" Plant Mol.Biol.25. 705-719 (1994)
[Publications] Walter A.,et al.: "Is the release of phytosiderophores in Zn-deficient wheat plants a response to impared iron utilization ?" Physiologia Plantarum. 92. 493-500 (1994)
[Publications] von Wiren N.,et al: "Iron Inefficiency in Maiz Mutant ys1(Zea mays L.ov.Yellow-Stripe)Is Caused by a Defect in Uptake of Iron Phytosiderophores" Plant Physiol.106. 71-77 (1994)
[Publications] Nishizawa N.,et.al.: "Ultraslructural Localization of the Basic 7S Globulin in Soybean(Glycine max)Cotyledons" Plant Cell Physiology. 35. 1079-1085 (1994)
[Publications] Nakamura S.,et al: "Detection and Characterization of Protein Kinases in Rice Phloem Sap" Plant Cell Physiol.36. 19-27 (1995)
[Publications] Mori S.: "Biochemistry of Metal Micronutrients in the Rhizosphere" Lowis Publishers, 24 (1994)
[Publications] Kanazawa K.,et al: "Iron Nutrion in Soil and Plants" Kluwer Academic Publishers, 6 (1994)

1994 Fiscal Year Annual Research Report

三価鉄還元酵素遺伝子導入(鉄欠乏耐性)植物の創成

Principal Investigator

森 敏 東京大学, 農学部, 教授 (90011915)

Research Products

[Publications] Higuchi K.,et al: "Purification and characterization of NA Synthase in the Roots of Gramineae" Plant and Soil. 165. 173-179 (1994)

[Publications] Okumura N.,et al.: "A dioxygenase gene(Ids2)expressed under iron deficiency conditions in the roots of Hordeum vulgare" Plant Mol.Biol.25. 705-719 (1994)

[Publications] Walter A.,et al.: "Is the release of phytosiderophores in Zn-deficient wheat plants a response to impared iron utilization ?" Physiologia Plantarum. 92. 493-500 (1994)

[Publications] von Wiren N.,et al: "Iron Inefficiency in Maiz Mutant ys1(Zea mays L.ov.Yellow-Stripe)Is Caused by a Defect in Uptake of Iron Phytosiderophores" Plant Physiol.106. 71-77 (1994)

[Publications] Nishizawa N.,et.al.: "Ultraslructural Localization of the Basic 7S Globulin in Soybean(Glycine max)Cotyledons" Plant Cell Physiology. 35. 1079-1085 (1994)

[Publications] Nakamura S.,et al: "Detection and Characterization of Protein Kinases in Rice Phloem Sap" Plant Cell Physiol.36. 19-27 (1995)

[Publications] Mori S.: "Biochemistry of Metal Micronutrients in the Rhizosphere" Lowis Publishers, 24 (1994)

[Publications] Kanazawa K.,et al: "Iron Nutrion in Soil and Plants" Kluwer Academic Publishers, 6 (1994)

森敏東京大学, 農学部, 教授 (90011915)