1996 Fiscal Year Final Research Report Summary
Introductory study on chemo-ecology of microbial flora in soil around plant roots.
| Project/Area Number |
06453173
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
FUKAMI Hiroshi Ritsumeikan University, Faculty of Science and Engineering, Professor, 理工学部, 教授 (60026531)
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| Co-Investigator(Kenkyū-buntansha) |
MORISAKI Hisao Ritsumeikan University, Faculty of Science and Engineering, Associate Professor, 理工学部, 助教授 (50125671)
TACHIKI Takashi Ritsumeikan University, Faculty of Science and Engineering, Professor, 理工学部, 教授 (60026573)
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| Project Period (FY) |
1994 – 1996
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| Keywords | Pseudomonas syringae / Fusarium oxysporum / Bacillus sp.KA-304 / LC / APCI-MS / Clerodendrins |
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| Research Abstract |
Successful investigation into dynamic changes of microbial flora in soil makes it possible to approach a scentific annalysis of the fertility of the soil. Introductory research for the ploblem has been done for these 3 year and the summary is as follows ;
1. The quantitative analysis of a small amount of the compound contained in complex mixture.
(1) The quantitative analysis of clerodendrins by using LC/APCI-MS (Atomospheric chemical ionization mass spectrometry joined to high performance liquid chromatography).
(2) The analysis of the seasonal change in quantity of clerodedrin A and B contained in leaves of Clerodendron trichonum.
2. Introductory research for the analses of dynamic changes micronial flora in soil.
(1) Analyzes of the relationship between the surface characteristics and the attachment force of mycrobial cells.
(1) Attachment of Pseudomonas syringea onto leaf surface of Italian ryegrass.
(2) Measurement of attachment force of microbial cells.
(2) As an example for the interaction between microorganisms themselves in soil, bacteria having Basidiomycetes-lytic activity were sought in soil.
(3) Trials of the quntitative analysis of Fusarium oxysporum in soil with monoclonal antibody.
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