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1996 Fiscal Year Final Research Report Summary

Gene expression of gonadotropin and its producing pituitary cells

Research Project

Project/Area Number 06454019
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionGunma University

Principal Investigator

KATO Yukio  Institute for Molecular and Cellular Regulation, Gunma University Associate Professor, 生体調節研究所, 助教授 (30114177)

Project Period (FY) 1994 – 1996
Keywordsreproduction / pituitary / hormone / gonadotropin / gene expression / transcription factor / DNA binding protein
Research Abstract

In this project that focused the molecular biological study on the gene expression of gonadotropin and its producing pituitary cells, following results were obtained.
1.The transcription factors, cJun and cFos, were cloned from the anterior pituitary.
2.The involvement in the GnRH-dependent modulation of mRNA levels of cJun and cFos were clarified by quantitative RT-PCR.
3.According to the identify of pituitary cJun and cFos, yeast two-hybrid system that could search the interaction factor (s) for cJun were performed. From the screening of 40,000 cDNA clones, a new pituitary transcription factor, ATF4, was obtained, revealing that pituitary cJun forms heterodimer with ATF4 and participates with modulation of pituitary gene expressions.
4.A pituitary specific transcription factor, Pit-1, that is essential for tissue specifc expression of GH gene as well as differentiation of pituitary somatoroph, mammotroph thyrotroph, was cloned from porcine pituitary and its nucleotide sequence was determined.
5.Yeast one-hybrid system to identify factors that recognize regulatory sequence of hormone genes were constructed and 25 clones were obtained as candidates. Re-transformation to yeast cells showed that they are false positives. The cloning is now in progress.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Chung,H.-O.: "Molecular cloning of c-jun and c-fos cDNAs from porcine anterior pituitary and their involvement in gonadotropin-releasing hormone stimulation" Mol.Cell.Endocrinol. 119. 75-82 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ma,E.: "Expression of GH,TSHb,LHb and FSHb genes during fetal pituitary development in the pig" Exp.Clin.Endocrinol.Diabat.104. 464-472 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inaba,T.: "In vitro and in vivo bioactivity of recombinant porcine follicle stimulating hromone expressed using the baculovirus expression system" Theriogenology. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Granz,S.: "Ontogeny of growth hormone and LHb-,FSHb- and a-subunit mRNA levels in the porcine fetal and neonatal anterior pituitary" J.Nueuroendocrinol.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤幸雄: "組換え体性腺刺激ホルモンの生産と応用" 獣医畜産新報. 49. 39-43 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤幸雄: "「ホルモンの分子生物学(日本比較内分泌学会編)」シリーズ第1巻 ホルモン分子生物学序説 第2章真核生物の遺伝子構造" 学会出版センター, 18 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤幸雄: "「ホルモンの分子生物学(日本比較内分泌学会編)」シリーズ第1巻 ホルモン分子生物学序説 第4章 基本的な制御配列と転写調節因子" 学会出版センター, 36 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤幸雄: "「ホルモンの分子生物学(日本比較内分泌学会編)」シリーズ第1巻 ホルモン分子生物学序説 (学会出版センター)付録:遺伝子操作の流れ図" 学会出版センター, 30 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤幸雄: "「ホルモンの分子生物学(日本比較内分泌学会編)」シリーズ第3巻 生殖とホルモン 第2章ゴナドトロピン" 学会出版センター(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chung, H.: "O.Molecular cloning of c-jun and c-fos cDNAs from porcine anterior pituitary and their involvement in gonadotropin-releasing hormone stimulation" Mol.Cell.Endocrinol. 119. 75-82 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ma, E.: "Expression of GH,TSHbeta, LHbetax and FSHbeta genes during fetal pituitary development in the pig" Exp.Clin.Endocrinol.Diabat.104. 464-472 (1966)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inaba, T.: "In vitro and in vivo bioactivity of recombinant porcine follicle stimulating hromone expressed using the baculovirus expression system" Theriogenology. 47. 491-499 (1977)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Granz, S.: "Ontogeny of growth hormone and LHbeta-, FSHbeta-and alpha-subunit mRNA levels in the porcine fetal and neonatal anterior pituitary" J.Nueuroendocrinol. (in press). (1977)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, Y.: "Production of recombinant gonadotropin" J.Veteriany Med.49. 39-43 (1966)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, Y.: "Gene structure of eukaryote" Gakkai-shupan Centar, Molecular Biology of Hormone. Vol 1. 18 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, Y.: "Regulatory elements and transcription factors" Gakkai-shupan Center, Molecular Biology of Hormone. Vol 1. 36 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, Y.: "Appendix : Strategy of molecular cloning" Gakkai-shupan Center, Molecular Biology of Hormone. Vol 1. 30 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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