1995 Fiscal Year Final Research Report Summary
C-type natriuretic peptie as a paracrine and an endocrine hormone
| Project/Area Number |
06454020
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物形態・構造
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| Research Institution | Ocean Research Institute University of Tokyo |
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Principal Investigator |
TAKEI Yoshio Ocean Research Institute, University of Tokyo, Associate professor, 海洋研究所, 助教授 (10129249)
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| Co-Investigator(Kenkyū-buntansha) |
HYODO Susumu College of Arts and Sciences, University of Tokyo, Assistant professor, 教養学部, 助手 (40222244)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Atrial notriuretic peptide / Ventricular natriuretic peptide / B-type natriuretic peptide / C-type natriuretic peptide / Eel, Anguilla japonica / Bullfrog, Rana catesbeiana / Osmoregulation / ANP receptors |
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| Research Abstract |
1. Plasma atrial and ventricular natriuretic peptide (ANP and VNP) levels increased transiently after transfer of eels from fresh water (FW) to seawater (SW). Injection of hypertonic solutions markedly increased plasma ANP and VNP levels, but blood volume expanasion was a much smaller secretagogue in the eel. ANP and VNP secretion increased in isolated, perfused eel heart after prefusate osmolality was increased These results show that osmotic requlation dominates volemic requlation for ANP and VNP secretion in the eel, and osmotic stimulus was perceived by cardiocytes themselves.
2. Whole-body autoradiography revealed dense accumulation of ^<125>I-eel ANP in the gill. Radio-ligand binding assay showed that most ANP receptors in the eel gill are clearance type NPR-C ; however, guanylate cyclase-coupled, CNP-specific NPR-B was also present in the gill of only FW eels. This is consistent with much less expression of NPR-B message in SW eels than in FW eels.
3. ANP inhibits drinking and intestinal absorption of water and sodium in both FW and eels. ANP also inhibits secretion of cortisol, a fish mineralocorticoid important for SW adaptation, and causes antidiuresis and natriuresis only in SW eels. It seems that ANP is a sodium extruding hormone in SW fish.
4. B-type natriuretic peptide (BNP) was isolated from the cardiac ventricle of bullfrog. Thus semiaquatic amphibians seem to have terapod-type BNP not fish-type VNP in their Ventriles.
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[Publications] 高島,片渕,柴崎,柏木,萩原,竹井,広瀬: "Cloning, properties, site-directed mutagenesis, analysis of subunit structure, tissne distribution and regulation of expression of the type-C eel natrinretic receptor" Eur. J. Biochem. 227. 673-680 (1995)
Description
「研究成果報告書概要(和文)」より
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[Publications] Katafuchi, T., Takashima, A., Kashiwagi, M., Hagiwara, H., Takei, Y.and Hirose, S.: "Cloning and expressin of eel natriuretic peptide receptor B (NPR-B) and its comparison with the mammalian counterparts" Eur, J.Biochem. 222. 835-842 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takei, Y., Takano, M., Itahara, Y., Watanabe, T.X., Nakajima, K., Conklin, D.J., Duff, D.W.and Oloson, K.R: "Rainbow trout ventricular natriuretic peptide : isolation, sequencing and determinatin of biological activity" Gen.Comp Endocrinol. 96. 420-426 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, N., Nse, Y., Kase, Y., Sasayama, Y., Takei, Y., Nagasawa, H., Watanabe, T.X., Nakajima, K.and Sakakibara, S: "Aminio acid sdquence of Sardine calcitonin and its hypocalcemic activity in rats" Zool.Sci. 11. 713-716 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Takashima, A., Katafuchi, T., Shibasaki, M., Kashiwagi, M., Hagiwara, H., Takei, Y., and Hirose, S: "Cloning, properties, site-directed mutagenesis analysis of the subunit structure, tissue distrbution and regulation of expression of the type-C eel natriuretic peptide receptor" Eur.J.Biochem. 227. 673-680 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kashiwagi, M., Katafuchi, T., Kato, A., Inuyama, H., Ito, T., Hagiwara, H., Takei, Y., and Hrose, S.: "Cloning and properties of a novel natriuretic peptide receptor, NPR-D" Eur.J.Biochem. 233. 102-109 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Sakaguchi, H., Suzuki, H., Hagiwara, H., Kaiya, H., Takei, Y., Ito, M., Shibabe, S.and Hirose, S.: "Whole-body autoradio-graphy and microautoradiography in eels after intra-arterial administratioin of 125I-eel ANP." Am.J.Physiol. (in press). (1996)
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「研究成果報告書概要(欧文)」より
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