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1995 Fiscal Year Final Research Report Summary

Energetics and selectivity of the accumulation of vanadium by ascidians

Research Project

Project/Area Number 06454027
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物生理・代謝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MICHIBATA Hitoshi  Hiroshima University, Faculty of Science, Professor, 理学部, 教授 (00111740)

Co-Investigator(Kenkyū-buntansha) UYAMA Taro  Hiroshima University, Faculty of Science, Assistant Professor, 理学部, 講師 (60232914)
MORIYAMA Yoshinori  Hiroshima University, Faculty of Science, Associate Professor, 理学部, 助教授 (10150658)
Project Period (FY) 1994 – 1995
KeywordsMarine organisms / Ascidians / Metal ions / Vanadium / Accululation / Proteins
Research Abstract

We found the highest concentration of 350 mM vanadium in the blood cells of Ascidia gemmata belonging to the suborder Phlebobranchia, which concentration corresponds 10^7 times higher than that in sea water.
On the other hand, considering the pathway of accululation of vanadium from seawater, it should be appropriate that some proteins participate in the pathway, although the results reported hitherto seem to indicate that vanadium is present in a free, noncomplexed form or associated with low molecular weight components in ascidians. We attempted to extract proteins that bind or associate with vanadium in ascidians. Consequently, using a combined technique of SDS-PAGE and atomic absorption spectrometry, we first succeeded to isolate at least four different types of vanadium-binding proteins, approx. 100kDa, 40kDa, 15kDa, and 12.5kDa and to analyze amico acid sequences of them partially.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Michibata,H.: "The mechanism of accumulation of vanadium by ascidians : Some progress towards an understanding of this unusual phenomenon." Zool.Sci.,in press.(1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi,M.: "Rapid increases in unmber of vanadocytes in the vanadium-rich ascidian,Ascidia sydneiensis samea,upon treatment of live animals with NH_4CI." J.Exp.Zool.,in press.(1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko,A.: "Localization,with monoclonal antibodies and detection of autonomous fluorescence,of blood cells in the tissues of the vanadium-rich ascidian Ascidia sydneiensis samea." Zool.Sci.,. 12. 733-739 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wuchiyama,J.: "Classification,based on autonomous fluorescence,of the blood cells of several ascidians that contain high levels of vanadium." Acta Zool.,. 76. 51-55 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyama,T.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian Ascidia sydneiensis samea." J.Exp.Zool.270. 148-154 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanamori,K.: "Raman spectroscopic study of the vanadium and sulphate in blood cells homogenates of the ascidian,Ascidia gemmata." J.Mar.Biol.Ass.U.K.,. 74. 279-286 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hitoshi Michibata: "The Mechanism of Accumulation of Vanadium by Ascidians : Some Progress towards an Understanding of this Unusual Phenomenon." Zool.Sci.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi, M., Nose, Y., Uyama, T., Moriyama, Y.and Michibata, H.: "Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatment of live animals with NH_4Cl." J.Exp.Zool.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko, A., Uyama, T., Moriyama, Y.and Michibata, H.: "Localization, with monoclonal antibodies and detection of autonomous fluorescence, of blood cells in the tissues of the vanadium-rich ascidian Ascidia sydneiensis samea." Zool.Sci.733-739. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wuchiyama, J.and Michibata, H.: "Classification, based on autonomous fluorescence, of the blood cells of several ascidians that contain high levels of vanadium." Acta Zool.76. 51-55 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyama, T., Moriyama, Y., Futai, M.and Michibata, H.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian Ascidia sydneiensis samea." J.Exp.Zool.270. 148-154 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanamori, K.and Michibata, H.: "Raman spectroscopic study of the vanvadium and sulphate in blood cells homogenates of the ascidian, Ascidia gemmata." J.Mar.Biol.Ass.U.K.74. 279-286 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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