1995 Fiscal Year Final Research Report Summary
Isolation and purification of antifeedant and antioviposition substances against the diamondback moth from a tropical plant.
| Project/Area Number |
06454062
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | Okayama University |
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Principal Investigator |
NAKASUJI Fusao Okayama University Faculty of Agriculture Professor, 農学部, 教授 (20109317)
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| Co-Investigator(Kenkyū-buntansha) |
KAJIYAMA Shin-ichiro Okayama University Faculty of Technology Assistant, 工学部, 助手 (20243496)
KOBAYASHI Akio Okayama University Faculty of Technology Professor, 工学部, 教授 (30115844)
TSUKUDA Ritsuko Okayama University Faculty of Agriculture Assistant, 農学部, 助手 (10032989)
FUJISAKI Kenji Okayama University Faculty of Agriculture Professor, 農学部, 教授 (10228976)
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| Project Period (FY) |
1994 – 1995
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| Keywords | substance of tropical plant / Andrographis paniculata / diamondback moth / Plutella xylostella / antifeedant / antioviposition |
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| Research Abstract |
In 1994, antifeedant activity of the acetone extract from A.paniculata against the 1st- and 4th-instar larvae of the diamondback moth (DBM), P.xylostella, was observed at 60 ppm and 500 ppm respectively. Antioviposition effect on the DBM females was also observed on caged cabbage treated with these extracts at 125 ppm.
In 1995, a series of fractionation of the aerial parts of A.paniculata guided by a no-choice bioassay test on the 4th-instar larvae of the DBM was conducted for the isolation of the hexane, ETOAc, BuOH and water layrs. Subsequent bioassay tests showed that the hexane layr is the most potent antifeedant against the DBM larvae. Futher fractionation of the hexane layr yielded 7 fractions. Fractions 5 and 6 were still effective even at a reduced dose (50 mug/leaf disc). Thus, fraction 5 was subsequently fractionated and 5 sub-fractions (F5-1 to F5-5) were obtained. Among those F5-2 was the most effective feeding deterrent at a minimum dose of 25mug/leaf disc. Through preparative TLC,an active compound (F526) was finally purified and isolated from F5-2. Further chemical analysis using other analytical procedures will be done to determine the chemical structure of this active compound.
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