1995 Fiscal Year Final Research Report Summary
Clarification of microscopic soil ecosystem of methane-producing bacteria and sulfate-reducing bacteria
| Project/Area Number |
06454072
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Tamagawa University |
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Principal Investigator |
TAKESHIMA Seini Tamagawa University, Faculty of Agriculture, Professor, 農学部, 教授 (10074353)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Hitoshi Tamagawa University, Faculty of Agriculture, Lecturer, 農学部, 講師 (50241047)
TSUJI Takashi Mitsubishi Kasei Institute of Life Sciences, Laboratory of Bioimages, Senior Res, 生命画像情報研究室, 主任研究員
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| Project Period (FY) |
1994 – 1995
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| Keywords | Paddy soil / methane-producing bacteria / Image analysis / Soil microbial ecology / microscopic ecology / fluorometry |
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| Research Abstract |
It is well known that the methane gas production in natural environments is largely controlled by the competition between methane-producing bacteria and sulfate-reducing bacteria. Therefore, in this study we developed a new image-detection system by fluorometry and microscopy for clarifying this competition in soil.
From April in 1995 through March in 1996 we obtained the following results : 1) Ecosystem of the methane producing-bacteria in paddy soil was clarified by measuring pH, Eh, methanegas production, and concentrations of S^<2-> and organic materials in model systems. 2) Two visualization methods for all living bacteria in soil were developed by using fluorescence probes, 5- (and 6-) sulfofluorescein diacetate and 6-carboxyfluoresceindiacetate acetoxymethy1 ester.3) Photographs of living methane-producing bacteria (Mathanosarcina sp.) in an artificial soil of the seaside of Tokyo Bay were taken by fluorescence microscopy. 4)Peaks of fluorescence spectra of the methane-producing bacteria were found to be 420nm and 460nm.
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