1995 Fiscal Year Final Research Report Summary
Regulation of secondary metabolism and cell differentiation by A-factor and protein phosphorylation in Streptomyces.
| Project/Area Number |
06454073
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HORINOUCHI Sueharu University of Tokyo, Biotechnology, Professor, 農学部, 教授 (80143410)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYAMA Makoto University of Tokyo, Biotechnology Center, Assoc.Prof., 生物生産工学研究センター, 助教授 (00208240)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Streptomycetes / Utoregulator / Antibiotic / Morphogenesis / Signal transduction |
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| Research Abstract |
(1) The A-factor recepor protein (ArpA) was purified from S.griseus and arpA was cloned by PCR.We also cloned two arpA homologs from S.coelicolor A3 (2), which probably controlled antibiotic production in this strain, as determined by the phenotypes of each of disruptants.
(2) Transcription of amfR,a regulatory gene for aerial mycelium formation in S.griseus, was controlled by A-factor. A protein able to bind to its promoter region was purified and its gene was cloned.
(3) An afsK homolog (afsK2) was cloned from S.griseus. Genetic studies showed that the afsK2 gene is involved in aerial mycelium formation under the conditons of high osmolality
(4) Upstream from afsA was present a gene encoding a protein similar to prokaryotic transcriptional factors. The expression of afsA therefore apeared to be controlled by this upstream gene.
(5) An A-factor-responsive transcriptional activator (Adp) that bindsto the strR promoter was purified to homogeneity. The molecular mass of Adp was 40 kDa.
(6) A tyrosine phosphatase gene was cloned from S.coelicolor A3 (2) as a gene that caused pigmentation in S.lividans. The phosphatase produced in E.coli showed a tyrosine phosphatase activity.
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